LOCUS BBa_K228113 4603 bp DNA linear 23-May-2024 DEFINITION AND GATE AraC + SupD + LacP +RBS(B0033)+T7ptag ACCESSION BBa_K228113 VERSION BBa_K228113.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 misc_feature 1288..1423 /label=BBa_K228001 stem_loop 1443..1486 /label=stem_loop misc_feature 1432..1511 /label=BBa_B0010 terminator 1432..1511 /label=BBa_B0010 stem_loop 1527..1546 /label=T7 TE misc_feature 1553..1553 /label=stop polyA_site 1547..1560 /label=polya misc_feature 1520..1560 /label=BBa_B0012 terminator 1520..1560 /label=BBa_B0012 misc_feature 1288..1560 /label=BBa_K228100 CDS 1569..1656 /label=end of LacI coding region (inactive) misc_feature 1569..1768 /label=BBa_R0010 misc_feature 1657..1694 /label=CAP binding site promoter 1705..1710 /label=-35 promoter 1729..1734 /label=-10 misc_feature 1734..1768 /label=LacI binding site misc_feature 1741..1741 /label=start promoter 1569..1768 /label=BBa_R0010 RBS 1777..1787 /label=RBS-4\Weaker RBS 1783..1786 /label=RBS misc_feature 1777..1787 /label=BBa_B0033 RBS 1777..1787 /label=BBa_B0033 CDS 1794..4466 /label=cds CDS 1794..4466 /label=BBa_K228000 stem_loop 4486..4529 /label=stem_loop misc_feature 4475..4554 /label=BBa_B0010 terminator 4475..4554 /label=BBa_B0010 stem_loop 4570..4589 /label=T7 TE misc_feature 4596..4596 /label=stop polyA_site 4590..4603 /label=polya misc_feature 4563..4603 /label=BBa_B0012 terminator 4563..4603 /label=BBa_B0012 misc_feature 1569..4603 /label=BBa_K228108 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagcaa ttcggagaga tgccggagcg gctgaacgga 1321 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg 1381 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gccaggcatc 1441 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1501 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1561 tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 1621 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 1681 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 1741 aattgtgagc ggataacaat ttcacacata ctagagtcac acaggactac tagatgacca 1801 tgattaccgt gcactagaat accattaaca ttgctaagaa cgacttctct gacatcgaac 1861 tggctgctat cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac 1921 agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg 1981 agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct ctcatcacta 2041 ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc 2101 gcggcaagcg cccgacagcc ttccagttcc tgtaggaaat caagccggaa gccgtagcgt 2161 acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg 2221 ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg 2281 accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc 2341 acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac 2401 tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta ggagtacgct 2461 gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa aatgctggcg 2521 tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag gctatcgcaa 2581 cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta gttcctccta 2641 agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt cctctggcgc 2701 tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac atgcctgagg 2761 tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag 2821 cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc cctgcgattg 2881 agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag gctctcaccg 2941 cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta 3001 tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag gccatctggt 3061 tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag 3121 gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag 3181 gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc 3241 ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc 3301 cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct 3361 ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt 3421 ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat gaggtaggtg 3481 gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta 3541 agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta 3601 ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac 3661 tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga 3721 cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa gataccattc 3781 agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag gctgctggat 3841 acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa 3901 tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg 3961 gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt 4021 ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc ggtcagttcc 4081 gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac aaacaggagt 4141 ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt aagactgtag 4201 tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac tccttcggta 4261 ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg gttgacacat 4321 atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag ttgcacgagt 4381 ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct 4441 tagagtcgga cttcgcgttc gcataatact agagccaggc atcaaataaa acgaaaggct 4501 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 4561 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //