##gff-version 3
##sequence-region BBa_K228113 1 4603
BBa_K228113	.	engineered_region	1288	1560	.	+	0	ID=BBa_K228100;Name=BBa_K228100
BBa_K228113	.	terminator	1520	1560	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228100
BBa_K228113	.	stem_loop	1527	1546	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228113	.	stop_codon	1553	1553	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228113	.	polyA_site	1547	1560	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228113	.	engineered_region	1520	1560	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228113	.	mature_transcript_region	1288	1423	.	+	0	ID=BBa_K228001;Name=BBa_K228001;Parent=BBa_K228100
BBa_K228113	.	terminator	1432	1511	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228100
BBa_K228113	.	stem_loop	1443	1486	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228113	.	engineered_region	1432	1511	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228113	.	engineered_region	1569	4603	.	+	0	ID=BBa_K228108;Name=BBa_K228108
BBa_K228113	.	promoter	1569	1768	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K228108
BBa_K228113	.	promoter	1705	1710	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K228113	.	CDS	1569	1656	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K228113	.	engineered_region	1569	1768	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K228113	.	non_covalent_binding_site	1657	1694	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K228113	.	promoter	1729	1734	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K228113	.	non_covalent_binding_site	1734	1768	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K228113	.	start_codon	1741	1741	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K228113	.	ribosome_entry_site	1777	1787	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_K228108
BBa_K228113	.	ribosome_entry_site	1777	1787	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K228113	.	ribosome_entry_site	1783	1786	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K228113	.	engineered_region	1777	1787	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K228113	.	CDS	1794	4466	.	+	0	ID=BBa_K228000;Name=BBa_K228000;Parent=BBa_K228108
BBa_K228113	.	CDS	1794	4466	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228113	.	terminator	4475	4554	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228108
BBa_K228113	.	stem_loop	4486	4529	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228113	.	engineered_region	4475	4554	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228113	.	terminator	4563	4603	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228108
BBa_K228113	.	stem_loop	4570	4589	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228113	.	stop_codon	4596	4596	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228113	.	polyA_site	4590	4603	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228113	.	engineered_region	4563	4603	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228113	.	terminator	1101	1141	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228113	.	stem_loop	1108	1127	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228113	.	stop_codon	1134	1134	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228113	.	polyA_site	1128	1141	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228113	.	engineered_region	1101	1141	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228113	.	terminator	1013	1092	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228113	.	stem_loop	1024	1067	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228113	.	engineered_region	1013	1092	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228113	.	promoter	1	1279	.	+	0	ID=BBa_K228009;Name=BBa_K228009
BBa_K228113	.	promoter	1150	1279	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228113	.	engineered_region	1	1141	.	+	0	ID=BBa_K228008;Name=BBa_K228008;Parent=BBa_K228009
BBa_K228113	.	operator	1	38	.	+	0	ID=annotation2019999;Name=P(Cat);Parent=BBa_K228008
BBa_K228113	.	promoter	4	9	.	+	0	ID=annotation2020000;Name=-35;Parent=BBa_K228008
BBa_K228113	.	CDS	65	940	.	+	0	ID=annotation2020006;Name=araC;Parent=BBa_K228008
BBa_K228113	.	engineered_region	1	38	.	+	0	ID=annotation2020002;Name=BBa_I14033;Parent=BBa_K228008
BBa_K228113	.	engineered_region	47	58	.	+	0	ID=annotation2020003;Name=BBa_B0034;Parent=BBa_K228008
BBa_K228113	.	engineered_region	980	1004	.	+	0	ID=annotation2020008;Name=barcode;Parent=BBa_K228008
BBa_K228113	.	engineered_region	1101	1141	.	+	0	ID=annotation2020011;Name=BBa_B0012;Parent=BBa_K228008
BBa_K228113	.	promoter	27	32	.	+	0	ID=annotation2020001;Name=-10;Parent=BBa_K228008
BBa_K228113	.	polyA_site	1128	1141	.	+	0	ID=annotation2020013;Name=polya;Parent=BBa_K228008
BBa_K228113	.	tag	941	973	.	+	0	ID=annotation2020007;Name=LVA;Parent=BBa_K228008
BBa_K228113	.	engineered_region	65	979	.	+	0	ID=annotation2020005;Name=BBa_C0080;Parent=BBa_K228008
BBa_K228113	.	engineered_region	47	1141	.	+	0	ID=annotation2020015;Name=BBa_K228006;Parent=BBa_K228008
BBa_K228113	.	stem_loop	1024	1067	.	+	0	ID=annotation2020010;Name=stem_loop;Parent=BBa_K228008
BBa_K228113	.	conserved_region	51	54	.	+	0	ID=annotation2020004;Name=conserved;Parent=BBa_K228008
BBa_K228113	.	engineered_region	1013	1092	.	+	0	ID=annotation2020009;Name=BBa_B0010;Parent=BBa_K228008
BBa_K228113	.	stop_codon	1134	1134	.	+	0	ID=annotation2020014;Name=stop;Parent=BBa_K228008
BBa_K228113	.	stem_loop	1108	1127	.	+	0	ID=annotation2020012;Name=T7 TE;Parent=BBa_K228008
BBa_K228113	.	engineered_region	1150	1279	.	+	0	ID=annotation2020016;Name=BBa_I13453;Parent=BBa_K228008
BBa_K228113	.	promoter	1150	1279	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
>BBa_K228113
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatc
agtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttc
acaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaa
ccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcct
cgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctg
tgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatcc
atcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagct
ccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatc
cgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaa
acccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatat
cacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacc
tttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgg
gcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgt
ttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatg
ccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccg
tttttttgggctagctactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtagg
ggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagc
ttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaac
cgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcg
caacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgt
ggaattgtgagcggataacaatttcacacatactagagtcacacaggactactagatgaccatgattaccgtgcactag
aataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccatt
acggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagat
gtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaag
atgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgt
aggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaac
cgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagct
aagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttg
tcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgt
aggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggt
caagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatct
ctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcg
tcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtac
aaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagt
ggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaa
tcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatc
agccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggc
gcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaagg
taaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttc
cctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggt
gggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagcta
taactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggta
ggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgaga
ttctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctga
gaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttca
gtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattg
attccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgag
cgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagat
aagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaat
acaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaa
agatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccacctt
cgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattc
cggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctga
tttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttg
aacctccgtgacatcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata