LOCUS BBa_K228114 4606 bp DNA linear 22-May-2024 DEFINITION AND GATE AraC + SupD + LacP +RBS(JB0031)+T7ptag ACCESSION BBa_K228114 VERSION BBa_K228114.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 misc_feature 1288..1423 /label=BBa_K228001 stem_loop 1443..1486 /label=stem_loop misc_feature 1432..1511 /label=BBa_B0010 terminator 1432..1511 /label=BBa_B0010 stem_loop 1527..1546 /label=T7 TE misc_feature 1553..1553 /label=stop polyA_site 1547..1560 /label=polya misc_feature 1520..1560 /label=BBa_B0012 terminator 1520..1560 /label=BBa_B0012 misc_feature 1288..1560 /label=BBa_K228100 CDS 1569..1656 /label=end of LacI coding region (inactive) misc_feature 1569..1768 /label=BBa_R0010 misc_feature 1657..1694 /label=CAP binding site promoter 1705..1710 /label=-35 promoter 1729..1734 /label=-10 misc_feature 1734..1768 /label=LacI binding site misc_feature 1741..1741 /label=start promoter 1569..1768 /label=BBa_R0010 misc_feature 1783..1786 /label=conserved RBS 1777..1790 /label=BBa_B0031 CDS 1797..4469 /label=cds CDS 1797..4469 /label=BBa_K228000 stem_loop 4489..4532 /label=stem_loop misc_feature 4478..4557 /label=BBa_B0010 terminator 4478..4557 /label=BBa_B0010 stem_loop 4573..4592 /label=T7 TE misc_feature 4599..4599 /label=stop polyA_site 4593..4606 /label=polya misc_feature 4566..4606 /label=BBa_B0012 terminator 4566..4606 /label=BBa_B0012 misc_feature 1569..4606 /label=BBa_K228109 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagcaa ttcggagaga tgccggagcg gctgaacgga 1321 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg 1381 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gccaggcatc 1441 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1501 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1561 tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 1621 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 1681 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 1741 aattgtgagc ggataacaat ttcacacata ctagagtcac acaggaaacc tactagatga 1801 ccatgattac cgtgcactag aataccatta acattgctaa gaacgacttc tctgacatcg 1861 aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt ttagctcgcg 1921 aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc cgcaagatgt 1981 ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag cctctcatca 2041 ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa gtgaaagcta 2101 agcgcggcaa gcgcccgaca gccttccagt tcctgtagga aatcaagccg gaagccgtag 2161 cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat acaaccgttc 2221 aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc 2281 gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac aagcgcgtag 2341 ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc 2401 tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat gtaggagtac 2461 gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc caaaatgctg 2521 gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct gaggctatcg 2581 caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc gtagttcctc 2641 ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt cgtcctctgg 2701 cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg 2761 aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc 2821 tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac atccctgcga 2881 ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct gaggctctca 2941 ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga caaggctcgc aagtctcgcc 3001 gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat aaggccatct 3061 ggttccctta caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc 3121 aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca atcggtaagg 3181 aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgat aaggttccgt 3241 tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct tgcgctaagt 3301 ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc cttgcgttct 3361 gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc cttccgctgg 3421 cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga gatgaggtag 3481 gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca ggacatctac gggattgttg 3541 ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat aacgaagtag 3601 ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg ggcactaagg 3661 cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag cgttcagtca 3721 tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg gaagatacca 3781 ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgaat caggctgctg 3841 gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct gcggttgaag 3901 caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa gataagaaga 3961 ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat ggtttccctg 4021 tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt 4081 tccgcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca cacaaacagg 4141 agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt cgtaagactg 4201 tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac gactccttcg 4261 gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca 4321 catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac cagttgcacg 4381 agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac ctccgtgaca 4441 tcttagagtc ggacttcgcg ttcgcataat actagagcca ggcatcaaat aaaacgaaag 4501 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 4561 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //