LOCUS BBa_K228116 4607 bp DNA linear 22-Nov-2024 DEFINITION AND GATE AraC + SupD + LacP +RBS(B0030)+T7ptag ACCESSION BBa_K228116 VERSION BBa_K228116.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 misc_feature 1288..1423 /label=BBa_K228001 stem_loop 1443..1486 /label=stem_loop misc_feature 1432..1511 /label=BBa_B0010 terminator 1432..1511 /label=BBa_B0010 stem_loop 1527..1546 /label=T7 TE misc_feature 1553..1553 /label=stop polyA_site 1547..1560 /label=polya misc_feature 1520..1560 /label=BBa_B0012 terminator 1520..1560 /label=BBa_B0012 misc_feature 1288..1560 /label=BBa_K228100 CDS 1569..1656 /label=end of LacI coding region (inactive) misc_feature 1569..1768 /label=BBa_R0010 misc_feature 1657..1694 /label=CAP binding site promoter 1705..1710 /label=-35 promoter 1729..1734 /label=-10 misc_feature 1734..1768 /label=LacI binding site misc_feature 1741..1741 /label=start promoter 1569..1768 /label=BBa_R0010 RBS 1777..1791 /label=RBS-1\Strong RBS 1784..1788 /label=RBS misc_feature 1777..1791 /label=BBa_B0030 RBS 1777..1791 /label=BBa_B0030 CDS 1798..4470 /label=cds CDS 1798..4470 /label=BBa_K228000 stem_loop 4490..4533 /label=stem_loop misc_feature 4479..4558 /label=BBa_B0010 terminator 4479..4558 /label=BBa_B0010 stem_loop 4574..4593 /label=T7 TE misc_feature 4600..4600 /label=stop polyA_site 4594..4607 /label=polya misc_feature 4567..4607 /label=BBa_B0012 terminator 4567..4607 /label=BBa_B0012 misc_feature 1569..4607 /label=BBa_K228101 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagcaa ttcggagaga tgccggagcg gctgaacgga 1321 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg 1381 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gccaggcatc 1441 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1501 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1561 tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 1621 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 1681 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 1741 aattgtgagc ggataacaat ttcacacata ctagagatta aagaggagaa atactagatg 1801 accatgatta ccgtgcacta gaataccatt aacattgcta agaacgactt ctctgacatc 1861 gaactggctg ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc 1921 gaacagttgg cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg 1981 tttgagcgtc aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc 2041 actaccctac tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct 2101 aagcgcggca agcgcccgac agccttccag ttcctgtagg aaatcaagcc ggaagccgta 2161 gcgtacatca ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt 2221 caggctgtag caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc 2281 cgtgaccttg aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta 2341 gggcacgtct acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt 2401 ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta 2461 cgctgcatcg agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct 2521 ggcgtagtag gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc 2581 gcaacccgtg caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct 2641 cctaagccgt ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg 2701 gcgctggtgc gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct 2761 gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc 2821 ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg 2881 attgagcgtg aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc 2941 accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc 3001 cgtatcagcc ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc 3061 tggttccctt acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg 3121 caaggtaacg atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag 3181 gaaggttact actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg 3241 ttccctgagc gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag 3301 tctccactgg agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc 3361 tgctttgagt acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg 3421 gcgtttgacg ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta 3481 ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt 3541 gctaagaaag tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta 3601 gttaccgtga ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag 3661 gcactggctg gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc 3721 atgacgctgg cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc 3781 attcagccag ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct 3841 ggatacatgg ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa 3901 gcaatgaact ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag 3961 actggagaga ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct 4021 gtgtggcagg aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag 4081 ttccgcttac agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag 4141 gagtctggta tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact 4201 gtagtgtggg cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc 4261 ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac 4321 acatatgagt cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac 4381 gagtctcaat tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac 4441 atcttagagt cggacttcgc gttcgcataa tactagagcc aggcatcaaa taaaacgaaa 4501 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 4561 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //