LOCUS BBa_K228117 4605 bp DNA linear 23-May-2024 DEFINITION AND GATE AraC + SupD + LacP +RBS(B0032)+T7ptag ACCESSION BBa_K228117 VERSION BBa_K228117.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 misc_feature 1288..1423 /label=BBa_K228001 stem_loop 1443..1486 /label=stem_loop misc_feature 1432..1511 /label=BBa_B0010 terminator 1432..1511 /label=BBa_B0010 stem_loop 1527..1546 /label=T7 TE misc_feature 1553..1553 /label=stop polyA_site 1547..1560 /label=polya misc_feature 1520..1560 /label=BBa_B0012 terminator 1520..1560 /label=BBa_B0012 misc_feature 1288..1560 /label=BBa_K228100 CDS 1569..1656 /label=end of LacI coding region (inactive) misc_feature 1569..1768 /label=BBa_R0010 misc_feature 1657..1694 /label=CAP binding site promoter 1705..1710 /label=-35 promoter 1729..1734 /label=-10 misc_feature 1734..1768 /label=LacI binding site misc_feature 1741..1741 /label=start promoter 1569..1768 /label=BBa_R0010 RBS 1777..1789 /label=RBS-3\Weak RBS 1783..1786 /label=RBS misc_feature 1777..1789 /label=BBa_B0032 RBS 1777..1789 /label=BBa_B0032 CDS 1796..4468 /label=cds CDS 1796..4468 /label=BBa_K228000 stem_loop 4488..4531 /label=stem_loop misc_feature 4477..4556 /label=BBa_B0010 terminator 4477..4556 /label=BBa_B0010 stem_loop 4572..4591 /label=T7 TE misc_feature 4598..4598 /label=stop polyA_site 4592..4605 /label=polya misc_feature 4565..4605 /label=BBa_B0012 terminator 4565..4605 /label=BBa_B0012 misc_feature 1569..4605 /label=BBa_K228102 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagcaa ttcggagaga tgccggagcg gctgaacgga 1321 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg 1381 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gccaggcatc 1441 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1501 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1561 tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 1621 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 1681 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 1741 aattgtgagc ggataacaat ttcacacata ctagagtcac acaggaaagt actagatgac 1801 catgattacc gtgcactaga ataccattaa cattgctaag aacgacttct ctgacatcga 1861 actggctgct atcccgttca acactctggc tgaccattac ggtgagcgtt tagctcgcga 1921 acagttggcc cttgagcatg agtcttacga gatgggtgaa gcacgcttcc gcaagatgtt 1981 tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct gccgccaagc ctctcatcac 2041 taccctactc cctaagatga ttgcacgcat caacgactgg tttgaggaag tgaaagctaa 2101 gcgcggcaag cgcccgacag ccttccagtt cctgtaggaa atcaagccgg aagccgtagc 2161 gtacatcacc attaagacca ctctggcttg cctaaccagt gctgacaata caaccgttca 2221 ggctgtagca agcgcaatcg gtcgggccat tgaggacgag gctcgcttcg gtcgtatccg 2281 tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa caactcaaca agcgcgtagg 2341 gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct gacatgctct ctaagggtct 2401 actcggtggc gaggcgtggt cttcgtggca taaggaagac tctattcatg taggagtacg 2461 ctgcatcgag atgctcattg agtcaaccgg aatggttagc ttacaccgcc aaaatgctgg 2521 cgtagtaggt caagactctg agactatcga actcgcacct gaatacgctg aggctatcgc 2581 aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc caaccttgcg tagttcctcc 2641 taagccgtgg actggcatta ctggtggtgg ctattgggct aacggtcgtc gtcctctggc 2701 gctggtgcgt actcacagta agaaagcact gatgcgctac gaagacgttt acatgcctga 2761 ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg aaaatcaaca agaaagtcct 2821 agcggtcgcc aacgtaatca ccaagtggaa gcattgtccg gtcgaggaca tccctgcgat 2881 tgagcgtgaa gaactcccga tgaaaccgga agacatcgac atgaatcctg aggctctcac 2941 cgcgtggaaa cgtgctgccg ctgctgtgta ccgcaaggac aaggctcgca agtctcgccg 3001 tatcagcctt gagttcatgc ttgagcaagc caataagttt gctaaccata aggccatctg 3061 gttcccttac aacatggact ggcgcggtcg tgtttacgct gtgtcaatgt tcaacccgca 3121 aggtaacgat atgaccaaag gactgcttac gctggcgaaa ggtaaaccaa tcggtaagga 3181 aggttactac tggctgaaaa tccacggtgc aaactgtgcg ggtgtcgata aggttccgtt 3241 ccctgagcgc atcaagttca ttgaggaaaa ccacgagaac atcatggctt gcgctaagtc 3301 tccactggag aacacttggt gggctgagca agattctccg ttctgcttcc ttgcgttctg 3361 ctttgagtac gctggggtac agcaccacgg cctgagctat aactgctccc ttccgctggc 3421 gtttgacggg tcttgctctg gcatccagca cttctccgcg atgctccgag atgaggtagg 3481 tggtcgcgcg gttaacttgc ttcctagtga aaccgttcag gacatctacg ggattgttgc 3541 taagaaagtc aacgagattc tacaagcaga cgcaatcaat gggaccgata acgaagtagt 3601 taccgtgacc gatgagaaca ctggtgaaat ctctgagaaa gtcaagctgg gcactaaggc 3661 actggctggt caatggctgg cttacggtgt tactcgcagt gtgactaagc gttcagtcat 3721 gacgctggct tacgggtcca aagagttcgg cttccgtcaa caagtgctgg aagataccat 3781 tcagccagct attgattccg gcaagggtct gatgttcact cagccgaatc aggctgctgg 3841 atacatggct aagctgattt gggaatctgt gagcgtgacg gtggtagctg cggttgaagc 3901 aatgaactgg cttaagtctg ctgctaagct gctggctgct gaggtcaaag ataagaagac 3961 tggagagatt cttcgcaagc gttgcgctgt gcattgggta actcctgatg gtttccctgt 4021 gtggcaggaa tacaagaagc ctattcagac gcgcttgaac ctgatgttcc tcggtcagtt 4081 ccgcttacag cctaccatta acaccaacaa agatagcgag attgatgcac acaaacagga 4141 gtctggtatc gctcctaact ttgtacacag ccaagacggt agccaccttc gtaagactgt 4201 agtgtgggca cacgagaagt acggaatcga atcttttgca ctgattcacg actccttcgg 4261 taccattccg gctgacgctg cgaacctgtt caaagcagtg cgcgaaacta tggttgacac 4321 atatgagtct tgtgatgtac tggctgattt ctacgaccag ttcgctgacc agttgcacga 4381 gtctcaattg gacaaaatgc cagcacttcc ggctaaaggt aacttgaacc tccgtgacat 4441 cttagagtcg gacttcgcgt tcgcataata ctagagccag gcatcaaata aaacgaaagg 4501 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 4561 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata 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