##gff-version 3
##sequence-region BBa_K228117 1 4605
BBa_K228117	.	engineered_region	1288	1560	.	+	0	ID=BBa_K228100;Name=BBa_K228100
BBa_K228117	.	terminator	1520	1560	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228100
BBa_K228117	.	stem_loop	1527	1546	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228117	.	stop_codon	1553	1553	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228117	.	polyA_site	1547	1560	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228117	.	engineered_region	1520	1560	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228117	.	mature_transcript_region	1288	1423	.	+	0	ID=BBa_K228001;Name=BBa_K228001;Parent=BBa_K228100
BBa_K228117	.	terminator	1432	1511	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228100
BBa_K228117	.	stem_loop	1443	1486	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228117	.	engineered_region	1432	1511	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228117	.	promoter	1	1279	.	+	0	ID=BBa_K228009;Name=BBa_K228009
BBa_K228117	.	promoter	1150	1279	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228117	.	engineered_region	1	1141	.	+	0	ID=BBa_K228008;Name=BBa_K228008;Parent=BBa_K228009
BBa_K228117	.	polyA_site	1128	1141	.	+	0	ID=annotation2020013;Name=polya;Parent=BBa_K228008
BBa_K228117	.	promoter	27	32	.	+	0	ID=annotation2020001;Name=-10;Parent=BBa_K228008
BBa_K228117	.	engineered_region	47	1141	.	+	0	ID=annotation2020015;Name=BBa_K228006;Parent=BBa_K228008
BBa_K228117	.	stem_loop	1024	1067	.	+	0	ID=annotation2020010;Name=stem_loop;Parent=BBa_K228008
BBa_K228117	.	conserved_region	51	54	.	+	0	ID=annotation2020004;Name=conserved;Parent=BBa_K228008
BBa_K228117	.	engineered_region	1013	1092	.	+	0	ID=annotation2020009;Name=BBa_B0010;Parent=BBa_K228008
BBa_K228117	.	stop_codon	1134	1134	.	+	0	ID=annotation2020014;Name=stop;Parent=BBa_K228008
BBa_K228117	.	stem_loop	1108	1127	.	+	0	ID=annotation2020012;Name=T7 TE;Parent=BBa_K228008
BBa_K228117	.	engineered_region	1150	1279	.	+	0	ID=annotation2020016;Name=BBa_I13453;Parent=BBa_K228008
BBa_K228117	.	engineered_region	1101	1141	.	+	0	ID=annotation2020011;Name=BBa_B0012;Parent=BBa_K228008
BBa_K228117	.	operator	1	38	.	+	0	ID=annotation2019999;Name=P(Cat);Parent=BBa_K228008
BBa_K228117	.	tag	941	973	.	+	0	ID=annotation2020007;Name=LVA;Parent=BBa_K228008
BBa_K228117	.	engineered_region	65	979	.	+	0	ID=annotation2020005;Name=BBa_C0080;Parent=BBa_K228008
BBa_K228117	.	promoter	4	9	.	+	0	ID=annotation2020000;Name=-35;Parent=BBa_K228008
BBa_K228117	.	CDS	65	940	.	+	0	ID=annotation2020006;Name=araC;Parent=BBa_K228008
BBa_K228117	.	engineered_region	1	38	.	+	0	ID=annotation2020002;Name=BBa_I14033;Parent=BBa_K228008
BBa_K228117	.	engineered_region	47	58	.	+	0	ID=annotation2020003;Name=BBa_B0034;Parent=BBa_K228008
BBa_K228117	.	engineered_region	980	1004	.	+	0	ID=annotation2020008;Name=barcode;Parent=BBa_K228008
BBa_K228117	.	promoter	1150	1279	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228117	.	terminator	1013	1092	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228117	.	stem_loop	1024	1067	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228117	.	engineered_region	1013	1092	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228117	.	terminator	1101	1141	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228117	.	stem_loop	1108	1127	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228117	.	stop_codon	1134	1134	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228117	.	polyA_site	1128	1141	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228117	.	engineered_region	1101	1141	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228117	.	engineered_region	1569	4605	.	+	0	ID=BBa_K228102;Name=BBa_K228102
BBa_K228117	.	terminator	4565	4605	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228102
BBa_K228117	.	stem_loop	4572	4591	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228117	.	stop_codon	4598	4598	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228117	.	polyA_site	4592	4605	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228117	.	engineered_region	4565	4605	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228117	.	promoter	1569	1768	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K228102
BBa_K228117	.	promoter	1705	1710	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K228117	.	CDS	1569	1656	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K228117	.	engineered_region	1569	1768	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K228117	.	promoter	1729	1734	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K228117	.	non_covalent_binding_site	1734	1768	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K228117	.	start_codon	1741	1741	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K228117	.	non_covalent_binding_site	1657	1694	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K228117	.	terminator	4477	4556	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228102
BBa_K228117	.	stem_loop	4488	4531	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228117	.	engineered_region	4477	4556	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228117	.	ribosome_entry_site	1777	1789	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K228102
BBa_K228117	.	engineered_region	1777	1789	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K228117	.	ribosome_entry_site	1783	1786	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K228117	.	ribosome_entry_site	1777	1789	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K228117	.	CDS	1796	4468	.	+	0	ID=BBa_K228000;Name=BBa_K228000;Parent=BBa_K228102
BBa_K228117	.	CDS	1796	4468	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
>BBa_K228117
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatc
agtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttc
acaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaa
ccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcct
cgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctg
tgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatcc
atcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagct
ccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatc
cgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaa
acccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatat
cacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacc
tttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgg
gcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgt
ttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatg
ccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccg
tttttttgggctagctactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtagg
ggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagc
ttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaac
cgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcg
caacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgt
ggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgaccatgattaccgtgcact
agaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgacca
ttacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaag
atgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactcccta
agatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcct
gtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaataca
accgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaag
ctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagt
tgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcat
gtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtag
gtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcat
ctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggt
cgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgt
acaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaa
gtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatg
aatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgta
tcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactg
gcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaa
ggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgt
tccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttg
gtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagc
tataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgagg
taggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacga
gattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctct
gagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgtt
cagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctat
tgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtg
agcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaag
ataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcagga
atacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaac
aaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccacc
ttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccat
tccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggct
gatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaact
tgaacctccgtgacatcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata