LOCUS BBa_K228120 4370 bp DNA linear 01-Jun-2024 DEFINITION AND GATE LacP+RBS(B0032)+T7ptag+LuxP+SupD ACCESSION BBa_K228120 VERSION BBa_K228120.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 misc_feature 1053..1188 /label=BBa_K228001 stem_loop 1208..1251 /label=stem_loop misc_feature 1197..1276 /label=BBa_B0010 terminator 1197..1276 /label=BBa_B0010 stem_loop 1292..1311 /label=T7 TE misc_feature 1318..1318 /label=stop polyA_site 1312..1325 /label=polya misc_feature 1285..1325 /label=BBa_B0012 terminator 1285..1325 /label=BBa_B0012 misc_feature 1053..1325 /label=BBa_K228100 misc_feature 1..1325 /label=BBa_K228011 CDS 1334..1421 /label=end of LacI coding region (inactive) misc_feature 1334..1533 /label=BBa_R0010 misc_feature 1422..1459 /label=CAP binding site promoter 1470..1475 /label=-35 promoter 1494..1499 /label=-10 misc_feature 1499..1533 /label=LacI binding site misc_feature 1506..1506 /label=start promoter 1334..1533 /label=BBa_R0010 RBS 1542..1554 /label=RBS-3\Weak RBS 1548..1551 /label=RBS misc_feature 1542..1554 /label=BBa_B0032 RBS 1542..1554 /label=BBa_B0032 CDS 1561..4233 /label=cds CDS 1561..4233 /label=BBa_K228000 stem_loop 4253..4296 /label=stem_loop misc_feature 4242..4321 /label=BBa_B0010 terminator 4242..4321 /label=BBa_B0010 stem_loop 4337..4356 /label=T7 TE misc_feature 4363..4363 /label=stop polyA_site 4357..4370 /label=polya misc_feature 4330..4370 /label=BBa_B0012 terminator 4330..4370 /label=BBa_B0012 misc_feature 1334..4370 /label=BBa_K228102 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agcaattcgg agagatgccg gagcggctga 1081 acggaccggt ctctaaaacc ggagtagggg caactctacc gggggttcaa atccccctct 1141 ctccgccact acagatcctt agcgaaagct aaggattttt tttaagctta ctagagccag 1201 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1261 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 1321 ttatatacta gagcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 1381 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 1441 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 1501 tgtggaattg tgagcggata acaatttcac acatactaga gtcacacagg aaagtactag 1561 atgaccatga ttaccgtgca ctagaatacc attaacattg ctaagaacga cttctctgac 1621 atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 1681 cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 1741 atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 1801 atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 1861 gctaagcgcg gcaagcgccc gacagccttc cagttcctgt aggaaatcaa gccggaagcc 1921 gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgctga caatacaacc 1981 gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 2041 atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 2101 gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 2161 ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 2221 gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 2281 gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 2341 atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 2401 cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 2461 ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 2521 cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 2581 gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 2641 gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 2701 ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct 2761 cgccgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 2821 atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 2881 ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 2941 aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 3001 ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 3061 aagtctccac tggagaacac ttggtgggct gagcaagatt ctccgttctg cttccttgcg 3121 ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 3181 ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 3241 gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 3301 gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 3361 gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 3421 aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca 3481 gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat 3541 accattcagc cagctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 3601 gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 3661 gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 3721 aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 3781 cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 3841 cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 3901 caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 3961 actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 4021 ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 4081 gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 4141 cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 4201 gacatcttag agtcggactt cgcgttcgca taatactaga gccaggcatc aaataaaacg 4261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 4321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //