LOCUS BBa_K228124 4369 bp DNA linear 26-Dec-2024 DEFINITION AND GATE LacP+RBS(J611127)+T7ptag+LuxP+SupD ACCESSION BBa_K228124 VERSION BBa_K228124.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 misc_feature 1053..1188 /label=BBa_K228001 stem_loop 1208..1251 /label=stem_loop misc_feature 1197..1276 /label=BBa_B0010 terminator 1197..1276 /label=BBa_B0010 stem_loop 1292..1311 /label=T7 TE misc_feature 1318..1318 /label=stop polyA_site 1312..1325 /label=polya misc_feature 1285..1325 /label=BBa_B0012 terminator 1285..1325 /label=BBa_B0012 misc_feature 1053..1325 /label=BBa_K228100 misc_feature 1..1325 /label=BBa_K228011 CDS 1334..1421 /label=end of LacI coding region (inactive) misc_feature 1334..1533 /label=BBa_R0010 misc_feature 1422..1459 /label=CAP binding site promoter 1470..1475 /label=-35 promoter 1494..1499 /label=-10 misc_feature 1499..1533 /label=LacI binding site misc_feature 1506..1506 /label=start promoter 1334..1533 /label=BBa_R0010 RBS 1542..1553 /label=BBa_J61127 CDS 1560..4232 /label=cds CDS 1560..4232 /label=BBa_K228000 stem_loop 4252..4295 /label=stem_loop misc_feature 4241..4320 /label=BBa_B0010 terminator 4241..4320 /label=BBa_B0010 stem_loop 4336..4355 /label=T7 TE misc_feature 4362..4362 /label=stop polyA_site 4356..4369 /label=polya misc_feature 4329..4369 /label=BBa_B0012 terminator 4329..4369 /label=BBa_B0012 misc_feature 1334..4369 /label=BBa_K228106 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agcaattcgg agagatgccg gagcggctga 1081 acggaccggt ctctaaaacc ggagtagggg caactctacc gggggttcaa atccccctct 1141 ctccgccact acagatcctt agcgaaagct aaggattttt tttaagctta ctagagccag 1201 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1261 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 1321 ttatatacta gagcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 1381 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 1441 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 1501 tgtggaattg tgagcggata acaatttcac acatactaga gaaagagtgg aactactaga 1561 tgaccatgat taccgtgcac tagaatacca ttaacattgc taagaacgac ttctctgaca 1621 tcgaactggc tgctatcccg ttcaacactc tggctgacca ttacggtgag cgtttagctc 1681 gcgaacagtt ggcccttgag catgagtctt acgagatggg tgaagcacgc ttccgcaaga 1741 tgtttgagcg tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc aagcctctca 1801 tcactaccct actccctaag atgattgcac gcatcaacga ctggtttgag gaagtgaaag 1861 ctaagcgcgg caagcgcccg acagccttcc agttcctgta ggaaatcaag ccggaagccg 1921 tagcgtacat caccattaag accactctgg cttgcctaac cagtgctgac aatacaaccg 1981 ttcaggctgt agcaagcgca atcggtcggg ccattgagga cgaggctcgc ttcggtcgta 2041 tccgtgacct tgaagctaag cacttcaaga aaaacgttga ggaacaactc aacaagcgcg 2101 tagggcacgt ctacaagaaa gcatttatgc aagttgtcga ggctgacatg ctctctaagg 2161 gtctactcgg tggcgaggcg tggtcttcgt ggcataagga agactctatt catgtaggag 2221 tacgctgcat cgagatgctc attgagtcaa ccggaatggt tagcttacac cgccaaaatg 2281 ctggcgtagt aggtcaagac tctgagacta tcgaactcgc acctgaatac gctgaggcta 2341 tcgcaacccg tgcaggtgcg ctggctggca tctctccgat gttccaacct tgcgtagttc 2401 ctcctaagcc gtggactggc attactggtg gtggctattg ggctaacggt cgtcgtcctc 2461 tggcgctggt gcgtactcac agtaagaaag cactgatgcg ctacgaagac gtttacatgc 2521 ctgaggtgta caaagcgatt aacattgcgc aaaacaccgc atggaaaatc aacaagaaag 2581 tcctagcggt cgccaacgta atcaccaagt ggaagcattg tccggtcgag gacatccctg 2641 cgattgagcg tgaagaactc ccgatgaaac cggaagacat cgacatgaat cctgaggctc 2701 tcaccgcgtg gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc 2761 gccgtatcag ccttgagttc atgcttgagc aagccaataa gtttgctaac cataaggcca 2821 tctggttccc ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc 2881 cgcaaggtaa cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta 2941 aggaaggtta ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc 3001 cgttccctga gcgcatcaag ttcattgagg aaaaccacga gaacatcatg gcttgcgcta 3061 agtctccact ggagaacact tggtgggctg agcaagattc tccgttctgc ttccttgcgt 3121 tctgctttga gtacgctggg gtacagcacc acggcctgag ctataactgc tcccttccgc 3181 tggcgtttga cgggtcttgc tctggcatcc agcacttctc cgcgatgctc cgagatgagg 3241 taggtggtcg cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc tacgggattg 3301 ttgctaagaa agtcaacgag attctacaag cagacgcaat caatgggacc gataacgaag 3361 tagttaccgt gaccgatgag aacactggtg aaatctctga gaaagtcaag ctgggcacta 3421 aggcactggc tggtcaatgg ctggcttacg gtgttactcg cagtgtgact aagcgttcag 3481 tcatgacgct ggcttacggg tccaaagagt tcggcttccg tcaacaagtg ctggaagata 3541 ccattcagcc agctattgat tccggcaagg gtctgatgtt cactcagccg aatcaggctg 3601 ctggatacat ggctaagctg atttgggaat ctgtgagcgt gacggtggta gctgcggttg 3661 aagcaatgaa ctggcttaag tctgctgcta agctgctggc tgctgaggtc aaagataaga 3721 agactggaga gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc 3781 ctgtgtggca ggaatacaag aagcctattc agacgcgctt gaacctgatg ttcctcggtc 3841 agttccgctt acagcctacc attaacacca acaaagatag cgagattgat gcacacaaac 3901 aggagtctgg tatcgctcct aactttgtac acagccaaga cggtagccac cttcgtaaga 3961 ctgtagtgtg ggcacacgag aagtacggaa tcgaatcttt tgcactgatt cacgactcct 4021 tcggtaccat tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg 4081 acacatatga gtcttgtgat gtactggctg atttctacga ccagttcgct gaccagttgc 4141 acgagtctca attggacaaa atgccagcac ttccggctaa aggtaacttg aacctccgtg 4201 acatcttaga gtcggacttc gcgttcgcat aatactagag ccaggcatca aataaaacga 4261 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 4321 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //