LOCUS BBa_K228126 4368 bp DNA linear 22-Nov-2024 DEFINITION AND GATE LacP+RBS(B0033)+T7ptag+LuxP+SupD ACCESSION BBa_K228126 VERSION BBa_K228126.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 misc_feature 1053..1188 /label=BBa_K228001 stem_loop 1208..1251 /label=stem_loop misc_feature 1197..1276 /label=BBa_B0010 terminator 1197..1276 /label=BBa_B0010 stem_loop 1292..1311 /label=T7 TE misc_feature 1318..1318 /label=stop polyA_site 1312..1325 /label=polya misc_feature 1285..1325 /label=BBa_B0012 terminator 1285..1325 /label=BBa_B0012 misc_feature 1053..1325 /label=BBa_K228100 misc_feature 1..1325 /label=BBa_K228011 CDS 1334..1421 /label=end of LacI coding region (inactive) misc_feature 1334..1533 /label=BBa_R0010 misc_feature 1422..1459 /label=CAP binding site promoter 1470..1475 /label=-35 promoter 1494..1499 /label=-10 misc_feature 1499..1533 /label=LacI binding site misc_feature 1506..1506 /label=start promoter 1334..1533 /label=BBa_R0010 RBS 1542..1552 /label=RBS-4\Weaker RBS 1548..1551 /label=RBS misc_feature 1542..1552 /label=BBa_B0033 RBS 1542..1552 /label=BBa_B0033 CDS 1559..4231 /label=cds CDS 1559..4231 /label=BBa_K228000 stem_loop 4251..4294 /label=stem_loop misc_feature 4240..4319 /label=BBa_B0010 terminator 4240..4319 /label=BBa_B0010 stem_loop 4335..4354 /label=T7 TE misc_feature 4361..4361 /label=stop polyA_site 4355..4368 /label=polya misc_feature 4328..4368 /label=BBa_B0012 terminator 4328..4368 /label=BBa_B0012 misc_feature 1334..4368 /label=BBa_K228108 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agcaattcgg agagatgccg gagcggctga 1081 acggaccggt ctctaaaacc ggagtagggg caactctacc gggggttcaa atccccctct 1141 ctccgccact acagatcctt agcgaaagct aaggattttt tttaagctta ctagagccag 1201 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1261 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 1321 ttatatacta gagcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 1381 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 1441 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 1501 tgtggaattg tgagcggata acaatttcac acatactaga gtcacacagg actactagat 1561 gaccatgatt accgtgcact agaataccat taacattgct aagaacgact tctctgacat 1621 cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg 1681 cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat 1741 gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat 1801 cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc 1861 taagcgcggc aagcgcccga cagccttcca gttcctgtag gaaatcaagc cggaagccgt 1921 agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt 1981 tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat 2041 ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt 2101 agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg 2161 tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt 2221 acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc 2281 tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat 2341 cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc 2401 tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct 2461 ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc 2521 tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt 2581 cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc 2641 gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc ctgaggctct 2701 caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg 2761 ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat 2821 ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc 2881 gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa 2941 ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc 3001 gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa 3061 gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt 3121 ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct 3181 ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt 3241 aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt 3301 tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt 3361 agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa 3421 ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt 3481 catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac 3541 cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc 3601 tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga 3661 agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa 3721 gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc 3781 tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca 3841 gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca 3901 ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac 3961 tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt 4021 cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga 4081 cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca 4141 cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga 4201 catcttagag tcggacttcg cgttcgcata atactagagc caggcatcaa ataaaacgaa 4261 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 4321 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //