LOCUS BBa_K228127 4371 bp DNA linear 22-Nov-2024 DEFINITION AND GATE LacP+RBS(B0031)+T7ptag+LuxP+SupD ACCESSION BBa_K228127 VERSION BBa_K228127.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 misc_feature 1053..1188 /label=BBa_K228001 stem_loop 1208..1251 /label=stem_loop misc_feature 1197..1276 /label=BBa_B0010 terminator 1197..1276 /label=BBa_B0010 stem_loop 1292..1311 /label=T7 TE misc_feature 1318..1318 /label=stop polyA_site 1312..1325 /label=polya misc_feature 1285..1325 /label=BBa_B0012 terminator 1285..1325 /label=BBa_B0012 misc_feature 1053..1325 /label=BBa_K228100 misc_feature 1..1325 /label=BBa_K228011 CDS 1334..1421 /label=end of LacI coding region (inactive) misc_feature 1334..1533 /label=BBa_R0010 misc_feature 1422..1459 /label=CAP binding site promoter 1470..1475 /label=-35 promoter 1494..1499 /label=-10 misc_feature 1499..1533 /label=LacI binding site misc_feature 1506..1506 /label=start promoter 1334..1533 /label=BBa_R0010 misc_feature 1548..1551 /label=conserved RBS 1542..1555 /label=BBa_B0031 CDS 1562..4234 /label=cds CDS 1562..4234 /label=BBa_K228000 stem_loop 4254..4297 /label=stem_loop misc_feature 4243..4322 /label=BBa_B0010 terminator 4243..4322 /label=BBa_B0010 stem_loop 4338..4357 /label=T7 TE misc_feature 4364..4364 /label=stop polyA_site 4358..4371 /label=polya misc_feature 4331..4371 /label=BBa_B0012 terminator 4331..4371 /label=BBa_B0012 misc_feature 1334..4371 /label=BBa_K228109 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agcaattcgg agagatgccg gagcggctga 1081 acggaccggt ctctaaaacc ggagtagggg caactctacc gggggttcaa atccccctct 1141 ctccgccact acagatcctt agcgaaagct aaggattttt tttaagctta ctagagccag 1201 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1261 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 1321 ttatatacta gagcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 1381 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 1441 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 1501 tgtggaattg tgagcggata acaatttcac acatactaga gtcacacagg aaacctacta 1561 gatgaccatg attaccgtgc actagaatac cattaacatt gctaagaacg acttctctga 1621 catcgaactg gctgctatcc cgttcaacac tctggctgac cattacggtg agcgtttagc 1681 tcgcgaacag ttggcccttg agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa 1741 gatgtttgag cgtcaactta aagctggtga ggttgcggat aacgctgccg ccaagcctct 1801 catcactacc ctactcccta agatgattgc acgcatcaac gactggtttg aggaagtgaa 1861 agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg taggaaatca agccggaagc 1921 cgtagcgtac atcaccatta agaccactct ggcttgccta accagtgctg acaatacaac 1981 cgttcaggct gtagcaagcg caatcggtcg ggccattgag gacgaggctc gcttcggtcg 2041 tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg 2101 cgtagggcac gtctacaaga aagcatttat gcaagttgtc gaggctgaca tgctctctaa 2161 gggtctactc ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg 2221 agtacgctgc atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa 2281 tgctggcgta gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc 2341 tatcgcaacc cgtgcaggtg cgctggctgg catctctccg atgttccaac cttgcgtagt 2401 tcctcctaag ccgtggactg gcattactgg tggtggctat tgggctaacg gtcgtcgtcc 2461 tctggcgctg gtgcgtactc acagtaagaa agcactgatg cgctacgaag acgtttacat 2521 gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa 2581 agtcctagcg gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg aggacatccc 2641 tgcgattgag cgtgaagaac tcccgatgaa accggaagac atcgacatga atcctgaggc 2701 tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc 2761 tcgccgtatc agccttgagt tcatgcttga gcaagccaat aagtttgcta accataaggc 2821 catctggttc ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa 2881 cccgcaaggt aacgatatga ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg 2941 taaggaaggt tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt 3001 tccgttccct gagcgcatca agttcattga ggaaaaccac gagaacatca tggcttgcgc 3061 taagtctcca ctggagaaca cttggtgggc tgagcaagat tctccgttct gcttccttgc 3121 gttctgcttt gagtacgctg gggtacagca ccacggcctg agctataact gctcccttcc 3181 gctggcgttt gacgggtctt gctctggcat ccagcacttc tccgcgatgc tccgagatga 3241 ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca tctacgggat 3301 tgttgctaag aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga 3361 agtagttacc gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac 3421 taaggcactg gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc 3481 agtcatgacg ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga 3541 taccattcag ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc 3601 tgctggatac atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt 3661 tgaagcaatg aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa 3721 gaagactgga gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt 3781 ccctgtgtgg caggaataca agaagcctat tcagacgcgc ttgaacctga tgttcctcgg 3841 tcagttccgc ttacagccta ccattaacac caacaaagat agcgagattg atgcacacaa 3901 acaggagtct ggtatcgctc ctaactttgt acacagccaa gacggtagcc accttcgtaa 3961 gactgtagtg tgggcacacg agaagtacgg aatcgaatct tttgcactga ttcacgactc 4021 cttcggtacc attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt 4081 tgacacatat gagtcttgtg atgtactggc tgatttctac gaccagttcg ctgaccagtt 4141 gcacgagtct caattggaca aaatgccagc acttccggct aaaggtaact tgaacctccg 4201 tgacatctta gagtcggact tcgcgttcgc ataatactag agccaggcat caaataaaac 4261 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 4321 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //