LOCUS       BBa_K228200             2831 bp    DNA     linear       17-May-2024
DEFINITION  RBS->T7ptag->Terminator
ACCESSION   BBa_K228200
VERSION     BBa_K228200.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..2694
                     /label=cds
     CDS             22..2694
                     /label=BBa_K228000
     stem_loop       2714..2757
                     /label=stem_loop
     misc_feature    2703..2782
                     /label=BBa_B0010
     terminator      2703..2782
                     /label=BBa_B0010
     stem_loop       2798..2817
                     /label=T7 TE
     misc_feature    2824..2824
                     /label=stop
     polyA_site      2818..2831
                     /label=polya
     misc_feature    2791..2831
                     /label=BBa_B0012
     terminator      2791..2831
                     /label=BBa_B0012
ORIGIN
        1 attaaagagg agaaatacta gatgaccatg attaccgtgc actagaatac cattaacatt
       61 gctaagaacg acttctctga catcgaactg gctgctatcc cgttcaacac tctggctgac
      121 cattacggtg agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg
      181 ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat
      241 aacgctgccg ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac
      301 gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg
      361 taggaaatca agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta
      421 accagtgctg acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag
      481 gacgaggctc gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt
      541 gaggaacaac tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc
      601 gaggctgaca tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag
      661 gaagactcta ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg
      721 gttagcttac accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc
      781 gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctccg
      841 atgttccaac cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat
      901 tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg
      961 cgctacgaag acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc
     1021 gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa gtggaagcat
     1081 tgtccggtcg aggacatccc tgcgattgag cgtgaagaac tcccgatgaa accggaagac
     1141 atcgacatga atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc
     1201 aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga gcaagccaat
     1261 aagtttgcta accataaggc catctggttc ccttacaaca tggactggcg cggtcgtgtt
     1321 tacgctgtgt caatgttcaa cccgcaaggt aacgatatga ccaaaggact gcttacgctg
     1381 gcgaaaggta aaccaatcgg taaggaaggt tactactggc tgaaaatcca cggtgcaaac
     1441 tgtgcgggtg tcgataaggt tccgttccct gagcgcatca agttcattga ggaaaaccac
     1501 gagaacatca tggcttgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat
     1561 tctccgttct gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg
     1621 agctataact gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc
     1681 tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc
     1741 gttcaggaca tctacgggat tgttgctaag aaagtcaacg agattctaca agcagacgca
     1801 atcaatggga ccgataacga agtagttacc gtgaccgatg agaacactgg tgaaatctct
     1861 gagaaagtca agctgggcac taaggcactg gctggtcaat ggctggctta cggtgttact
     1921 cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga gttcggcttc
     1981 cgtcaacaag tgctggaaga taccattcag ccagctattg attccggcaa gggtctgatg
     2041 ttcactcagc cgaatcaggc tgctggatac atggctaagc tgatttggga atctgtgagc
     2101 gtgacggtgg tagctgcggt tgaagcaatg aactggctta agtctgctgc taagctgctg
     2161 gctgctgagg tcaaagataa gaagactgga gagattcttc gcaagcgttg cgctgtgcat
     2221 tgggtaactc ctgatggttt ccctgtgtgg caggaataca agaagcctat tcagacgcgc
     2281 ttgaacctga tgttcctcgg tcagttccgc ttacagccta ccattaacac caacaaagat
     2341 agcgagattg atgcacacaa acaggagtct ggtatcgctc ctaactttgt acacagccaa
     2401 gacggtagcc accttcgtaa gactgtagtg tgggcacacg agaagtacgg aatcgaatct
     2461 tttgcactga ttcacgactc cttcggtacc attccggctg acgctgcgaa cctgttcaaa
     2521 gcagtgcgcg aaactatggt tgacacatat gagtcttgtg atgtactggc tgatttctac
     2581 gaccagttcg ctgaccagtt gcacgagtct caattggaca aaatgccagc acttccggct
     2641 aaaggtaact tgaacctccg tgacatctta gagtcggact tcgcgttcgc ataatactag
     2701 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     2761 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     2821 ctgcgtttat a
//