LOCUS       BBa_K228202             2829 bp    DNA     linear       22-Dec-2024
DEFINITION  RBS->T7ptag->Terminator
ACCESSION   BBa_K228202
VERSION     BBa_K228202.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..2692
                     /label=cds
     CDS             20..2692
                     /label=BBa_K228000
     stem_loop       2712..2755
                     /label=stem_loop
     misc_feature    2701..2780
                     /label=BBa_B0010
     terminator      2701..2780
                     /label=BBa_B0010
     stem_loop       2796..2815
                     /label=T7 TE
     misc_feature    2822..2822
                     /label=stop
     polyA_site      2816..2829
                     /label=polya
     misc_feature    2789..2829
                     /label=BBa_B0012
     terminator      2789..2829
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aagtactaga tgaccatgat taccgtgcac tagaatacca ttaacattgc
       61 taagaacgac ttctctgaca tcgaactggc tgctatcccg ttcaacactc tggctgacca
      121 ttacggtgag cgtttagctc gcgaacagtt ggcccttgag catgagtctt acgagatggg
      181 tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa gctggtgagg ttgcggataa
      241 cgctgccgcc aagcctctca tcactaccct actccctaag atgattgcac gcatcaacga
      301 ctggtttgag gaagtgaaag ctaagcgcgg caagcgcccg acagccttcc agttcctgta
      361 ggaaatcaag ccggaagccg tagcgtacat caccattaag accactctgg cttgcctaac
      421 cagtgctgac aatacaaccg ttcaggctgt agcaagcgca atcggtcggg ccattgagga
      481 cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag cacttcaaga aaaacgttga
      541 ggaacaactc aacaagcgcg tagggcacgt ctacaagaaa gcatttatgc aagttgtcga
      601 ggctgacatg ctctctaagg gtctactcgg tggcgaggcg tggtcttcgt ggcataagga
      661 agactctatt catgtaggag tacgctgcat cgagatgctc attgagtcaa ccggaatggt
      721 tagcttacac cgccaaaatg ctggcgtagt aggtcaagac tctgagacta tcgaactcgc
      781 acctgaatac gctgaggcta tcgcaacccg tgcaggtgcg ctggctggca tctctccgat
      841 gttccaacct tgcgtagttc ctcctaagcc gtggactggc attactggtg gtggctattg
      901 ggctaacggt cgtcgtcctc tggcgctggt gcgtactcac agtaagaaag cactgatgcg
      961 ctacgaagac gtttacatgc ctgaggtgta caaagcgatt aacattgcgc aaaacaccgc
     1021 atggaaaatc aacaagaaag tcctagcggt cgccaacgta atcaccaagt ggaagcattg
     1081 tccggtcgag gacatccctg cgattgagcg tgaagaactc ccgatgaaac cggaagacat
     1141 cgacatgaat cctgaggctc tcaccgcgtg gaaacgtgct gccgctgctg tgtaccgcaa
     1201 ggacaaggct cgcaagtctc gccgtatcag ccttgagttc atgcttgagc aagccaataa
     1261 gtttgctaac cataaggcca tctggttccc ttacaacatg gactggcgcg gtcgtgttta
     1321 cgctgtgtca atgttcaacc cgcaaggtaa cgatatgacc aaaggactgc ttacgctggc
     1381 gaaaggtaaa ccaatcggta aggaaggtta ctactggctg aaaatccacg gtgcaaactg
     1441 tgcgggtgtc gataaggttc cgttccctga gcgcatcaag ttcattgagg aaaaccacga
     1501 gaacatcatg gcttgcgcta agtctccact ggagaacact tggtgggctg agcaagattc
     1561 tccgttctgc ttccttgcgt tctgctttga gtacgctggg gtacagcacc acggcctgag
     1621 ctataactgc tcccttccgc tggcgtttga cgggtcttgc tctggcatcc agcacttctc
     1681 cgcgatgctc cgagatgagg taggtggtcg cgcggttaac ttgcttccta gtgaaaccgt
     1741 tcaggacatc tacgggattg ttgctaagaa agtcaacgag attctacaag cagacgcaat
     1801 caatgggacc gataacgaag tagttaccgt gaccgatgag aacactggtg aaatctctga
     1861 gaaagtcaag ctgggcacta aggcactggc tggtcaatgg ctggcttacg gtgttactcg
     1921 cagtgtgact aagcgttcag tcatgacgct ggcttacggg tccaaagagt tcggcttccg
     1981 tcaacaagtg ctggaagata ccattcagcc agctattgat tccggcaagg gtctgatgtt
     2041 cactcagccg aatcaggctg ctggatacat ggctaagctg atttgggaat ctgtgagcgt
     2101 gacggtggta gctgcggttg aagcaatgaa ctggcttaag tctgctgcta agctgctggc
     2161 tgctgaggtc aaagataaga agactggaga gattcttcgc aagcgttgcg ctgtgcattg
     2221 ggtaactcct gatggtttcc ctgtgtggca ggaatacaag aagcctattc agacgcgctt
     2281 gaacctgatg ttcctcggtc agttccgctt acagcctacc attaacacca acaaagatag
     2341 cgagattgat gcacacaaac aggagtctgg tatcgctcct aactttgtac acagccaaga
     2401 cggtagccac cttcgtaaga ctgtagtgtg ggcacacgag aagtacggaa tcgaatcttt
     2461 tgcactgatt cacgactcct tcggtaccat tccggctgac gctgcgaacc tgttcaaagc
     2521 agtgcgcgaa actatggttg acacatatga gtcttgtgat gtactggctg atttctacga
     2581 ccagttcgct gaccagttgc acgagtctca attggacaaa atgccagcac ttccggctaa
     2641 aggtaacttg aacctccgtg acatcttaga gtcggacttc gcgttcgcat aatactagag
     2701 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     2761 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     2821 gcgtttata
//