LOCUS       BBa_K228203             2827 bp    DNA     linear       22-Nov-2024
DEFINITION  RBS->T7ptag->Terminator
ACCESSION   BBa_K228203
VERSION     BBa_K228203.1  
FEATURES             Location/Qualifiers
     RBS             1..11
                     /label=RBS-4\Weaker
     RBS             7..10
                     /label=RBS
     misc_feature    1..11
                     /label=BBa_B0033
     RBS             1..11
                     /label=BBa_B0033
     CDS             18..2690
                     /label=cds
     CDS             18..2690
                     /label=BBa_K228000
     stem_loop       2710..2753
                     /label=stem_loop
     misc_feature    2699..2778
                     /label=BBa_B0010
     terminator      2699..2778
                     /label=BBa_B0010
     stem_loop       2794..2813
                     /label=T7 TE
     misc_feature    2820..2820
                     /label=stop
     polyA_site      2814..2827
                     /label=polya
     misc_feature    2787..2827
                     /label=BBa_B0012
     terminator      2787..2827
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga ctactagatg accatgatta ccgtgcacta gaataccatt aacattgcta
       61 agaacgactt ctctgacatc gaactggctg ctatcccgtt caacactctg gctgaccatt
      121 acggtgagcg tttagctcgc gaacagttgg cccttgagca tgagtcttac gagatgggtg
      181 aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt gcggataacg
      241 ctgccgccaa gcctctcatc actaccctac tccctaagat gattgcacgc atcaacgact
      301 ggtttgagga agtgaaagct aagcgcggca agcgcccgac agccttccag ttcctgtagg
      361 aaatcaagcc ggaagccgta gcgtacatca ccattaagac cactctggct tgcctaacca
      421 gtgctgacaa tacaaccgtt caggctgtag caagcgcaat cggtcgggcc attgaggacg
      481 aggctcgctt cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa aacgttgagg
      541 aacaactcaa caagcgcgta gggcacgtct acaagaaagc atttatgcaa gttgtcgagg
      601 ctgacatgct ctctaagggt ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag
      661 actctattca tgtaggagta cgctgcatcg agatgctcat tgagtcaacc ggaatggtta
      721 gcttacaccg ccaaaatgct ggcgtagtag gtcaagactc tgagactatc gaactcgcac
      781 ctgaatacgc tgaggctatc gcaacccgtg caggtgcgct ggctggcatc tctccgatgt
      841 tccaaccttg cgtagttcct cctaagccgt ggactggcat tactggtggt ggctattggg
      901 ctaacggtcg tcgtcctctg gcgctggtgc gtactcacag taagaaagca ctgatgcgct
      961 acgaagacgt ttacatgcct gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat
     1021 ggaaaatcaa caagaaagtc ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc
     1081 cggtcgagga catccctgcg attgagcgtg aagaactccc gatgaaaccg gaagacatcg
     1141 acatgaatcc tgaggctctc accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg
     1201 acaaggctcg caagtctcgc cgtatcagcc ttgagttcat gcttgagcaa gccaataagt
     1261 ttgctaacca taaggccatc tggttccctt acaacatgga ctggcgcggt cgtgtttacg
     1321 ctgtgtcaat gttcaacccg caaggtaacg atatgaccaa aggactgctt acgctggcga
     1381 aaggtaaacc aatcggtaag gaaggttact actggctgaa aatccacggt gcaaactgtg
     1441 cgggtgtcga taaggttccg ttccctgagc gcatcaagtt cattgaggaa aaccacgaga
     1501 acatcatggc ttgcgctaag tctccactgg agaacacttg gtgggctgag caagattctc
     1561 cgttctgctt ccttgcgttc tgctttgagt acgctggggt acagcaccac ggcctgagct
     1621 ataactgctc ccttccgctg gcgtttgacg ggtcttgctc tggcatccag cacttctccg
     1681 cgatgctccg agatgaggta ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc
     1741 aggacatcta cgggattgtt gctaagaaag tcaacgagat tctacaagca gacgcaatca
     1801 atgggaccga taacgaagta gttaccgtga ccgatgagaa cactggtgaa atctctgaga
     1861 aagtcaagct gggcactaag gcactggctg gtcaatggct ggcttacggt gttactcgca
     1921 gtgtgactaa gcgttcagtc atgacgctgg cttacgggtc caaagagttc ggcttccgtc
     1981 aacaagtgct ggaagatacc attcagccag ctattgattc cggcaagggt ctgatgttca
     2041 ctcagccgaa tcaggctgct ggatacatgg ctaagctgat ttgggaatct gtgagcgtga
     2101 cggtggtagc tgcggttgaa gcaatgaact ggcttaagtc tgctgctaag ctgctggctg
     2161 ctgaggtcaa agataagaag actggagaga ttcttcgcaa gcgttgcgct gtgcattggg
     2221 taactcctga tggtttccct gtgtggcagg aatacaagaa gcctattcag acgcgcttga
     2281 acctgatgtt cctcggtcag ttccgcttac agcctaccat taacaccaac aaagatagcg
     2341 agattgatgc acacaaacag gagtctggta tcgctcctaa ctttgtacac agccaagacg
     2401 gtagccacct tcgtaagact gtagtgtggg cacacgagaa gtacggaatc gaatcttttg
     2461 cactgattca cgactccttc ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag
     2521 tgcgcgaaac tatggttgac acatatgagt cttgtgatgt actggctgat ttctacgacc
     2581 agttcgctga ccagttgcac gagtctcaat tggacaaaat gccagcactt ccggctaaag
     2641 gtaacttgaa cctccgtgac atcttagagt cggacttcgc gttcgcataa tactagagcc
     2701 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2761 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2821 gtttata
//