LOCUS       BBa_K228207             2828 bp    DNA     linear       29-Nov-2024
DEFINITION  RBS->T7ptag->Terminator
ACCESSION   BBa_K228207
VERSION     BBa_K228207.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=BBa_J61107
     CDS             19..2691
                     /label=cds
     CDS             19..2691
                     /label=BBa_K228000
     stem_loop       2711..2754
                     /label=stem_loop
     misc_feature    2700..2779
                     /label=BBa_B0010
     terminator      2700..2779
                     /label=BBa_B0010
     stem_loop       2795..2814
                     /label=T7 TE
     misc_feature    2821..2821
                     /label=stop
     polyA_site      2815..2828
                     /label=polya
     misc_feature    2788..2828
                     /label=BBa_B0012
     terminator      2788..2828
                     /label=BBa_B0012
ORIGIN
        1 aaagaagaga cttactagat gaccatgatt accgtgcact agaataccat taacattgct
       61 aagaacgact tctctgacat cgaactggct gctatcccgt tcaacactct ggctgaccat
      121 tacggtgagc gtttagctcg cgaacagttg gcccttgagc atgagtctta cgagatgggt
      181 gaagcacgct tccgcaagat gtttgagcgt caacttaaag ctggtgaggt tgcggataac
      241 gctgccgcca agcctctcat cactacccta ctccctaaga tgattgcacg catcaacgac
      301 tggtttgagg aagtgaaagc taagcgcggc aagcgcccga cagccttcca gttcctgtag
      361 gaaatcaagc cggaagccgt agcgtacatc accattaaga ccactctggc ttgcctaacc
      421 agtgctgaca atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac
      481 gaggctcgct tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa aaacgttgag
      541 gaacaactca acaagcgcgt agggcacgtc tacaagaaag catttatgca agttgtcgag
      601 gctgacatgc tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa
      661 gactctattc atgtaggagt acgctgcatc gagatgctca ttgagtcaac cggaatggtt
      721 agcttacacc gccaaaatgc tggcgtagta ggtcaagact ctgagactat cgaactcgca
      781 cctgaatacg ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg
      841 ttccaacctt gcgtagttcc tcctaagccg tggactggca ttactggtgg tggctattgg
      901 gctaacggtc gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc
      961 tacgaagacg tttacatgcc tgaggtgtac aaagcgatta acattgcgca aaacaccgca
     1021 tggaaaatca acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt
     1081 ccggtcgagg acatccctgc gattgagcgt gaagaactcc cgatgaaacc ggaagacatc
     1141 gacatgaatc ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag
     1201 gacaaggctc gcaagtctcg ccgtatcagc cttgagttca tgcttgagca agccaataag
     1261 tttgctaacc ataaggccat ctggttccct tacaacatgg actggcgcgg tcgtgtttac
     1321 gctgtgtcaa tgttcaaccc gcaaggtaac gatatgacca aaggactgct tacgctggcg
     1381 aaaggtaaac caatcggtaa ggaaggttac tactggctga aaatccacgg tgcaaactgt
     1441 gcgggtgtcg ataaggttcc gttccctgag cgcatcaagt tcattgagga aaaccacgag
     1501 aacatcatgg cttgcgctaa gtctccactg gagaacactt ggtgggctga gcaagattct
     1561 ccgttctgct tccttgcgtt ctgctttgag tacgctgggg tacagcacca cggcctgagc
     1621 tataactgct cccttccgct ggcgtttgac gggtcttgct ctggcatcca gcacttctcc
     1681 gcgatgctcc gagatgaggt aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt
     1741 caggacatct acgggattgt tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc
     1801 aatgggaccg ataacgaagt agttaccgtg accgatgaga acactggtga aatctctgag
     1861 aaagtcaagc tgggcactaa ggcactggct ggtcaatggc tggcttacgg tgttactcgc
     1921 agtgtgacta agcgttcagt catgacgctg gcttacgggt ccaaagagtt cggcttccgt
     1981 caacaagtgc tggaagatac cattcagcca gctattgatt ccggcaaggg tctgatgttc
     2041 actcagccga atcaggctgc tggatacatg gctaagctga tttgggaatc tgtgagcgtg
     2101 acggtggtag ctgcggttga agcaatgaac tggcttaagt ctgctgctaa gctgctggct
     2161 gctgaggtca aagataagaa gactggagag attcttcgca agcgttgcgc tgtgcattgg
     2221 gtaactcctg atggtttccc tgtgtggcag gaatacaaga agcctattca gacgcgcttg
     2281 aacctgatgt tcctcggtca gttccgctta cagcctacca ttaacaccaa caaagatagc
     2341 gagattgatg cacacaaaca ggagtctggt atcgctccta actttgtaca cagccaagac
     2401 ggtagccacc ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat cgaatctttt
     2461 gcactgattc acgactcctt cggtaccatt ccggctgacg ctgcgaacct gttcaaagca
     2521 gtgcgcgaaa ctatggttga cacatatgag tcttgtgatg tactggctga tttctacgac
     2581 cagttcgctg accagttgca cgagtctcaa ttggacaaaa tgccagcact tccggctaaa
     2641 ggtaacttga acctccgtga catcttagag tcggacttcg cgttcgcata atactagagc
     2701 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2761 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2821 cgtttata
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