LOCUS BBa_K228209 4118 bp DNA linear 22-Dec-2024 DEFINITION AraC->rbs->T7ptag->Terminator ACCESSION BBa_K228209 VERSION BBa_K228209.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 RBS 1288..1302 /label=RBS-1\Strong RBS 1295..1299 /label=RBS misc_feature 1288..1302 /label=BBa_B0030 RBS 1288..1302 /label=BBa_B0030 CDS 1309..3981 /label=cds CDS 1309..3981 /label=BBa_K228000 stem_loop 4001..4044 /label=stem_loop misc_feature 3990..4069 /label=BBa_B0010 terminator 3990..4069 /label=BBa_B0010 stem_loop 4085..4104 /label=T7 TE misc_feature 4111..4111 /label=stop polyA_site 4105..4118 /label=polya misc_feature 4078..4118 /label=BBa_B0012 terminator 4078..4118 /label=BBa_B0012 misc_feature 1288..4118 /label=BBa_K228200 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagatt aaagaggaga aatactagat gaccatgatt 1321 accgtgcact agaataccat taacattgct aagaacgact tctctgacat cgaactggct 1381 gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg cgaacagttg 1441 gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat gtttgagcgt 1501 caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat cactacccta 1561 ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc taagcgcggc 1621 aagcgcccga cagccttcca gttcctgtag gaaatcaagc cggaagccgt agcgtacatc 1681 accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt tcaggctgta 1741 gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat ccgtgacctt 1801 gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt agggcacgtc 1861 tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg tctactcggt 1921 ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt acgctgcatc 1981 gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc tggcgtagta 2041 ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat cgcaacccgt 2101 gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc tcctaagccg 2161 tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct ggcgctggtg 2221 cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc tgaggtgtac 2281 aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt cctagcggtc 2341 gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc gattgagcgt 2401 gaagaactcc cgatgaaacc ggaagacatc gacatgaatc ctgaggctct caccgcgtgg 2461 aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg ccgtatcagc 2521 cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat ctggttccct 2581 tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc gcaaggtaac 2641 gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa ggaaggttac 2701 tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc gttccctgag 2761 cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa gtctccactg 2821 gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt ctgctttgag 2881 tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct ggcgtttgac 2941 gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt aggtggtcgc 3001 gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt tgctaagaaa 3061 gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt agttaccgtg 3121 accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa ggcactggct 3181 ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt catgacgctg 3241 gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac cattcagcca 3301 gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc tggatacatg 3361 gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga agcaatgaac 3421 tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa gactggagag 3481 attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc tgtgtggcag 3541 gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca gttccgctta 3601 cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca ggagtctggt 3661 atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac tgtagtgtgg 3721 gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt cggtaccatt 3781 ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga cacatatgag 3841 tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca cgagtctcaa 3901 ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga catcttagag 3961 tcggacttcg cgttcgcata atactagagc caggcatcaa ataaaacgaa aggctcagtc 4021 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 4081 cactggctca ccttcgggtg ggcctttctg cgtttata //