LOCUS BBa_K228211 4116 bp DNA linear 23-May-2024 DEFINITION AraC->rbs->T7ptag->Terminator ACCESSION BBa_K228211 VERSION BBa_K228211.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 RBS 1288..1300 /label=RBS-3\Weak RBS 1294..1297 /label=RBS misc_feature 1288..1300 /label=BBa_B0032 RBS 1288..1300 /label=BBa_B0032 CDS 1307..3979 /label=cds CDS 1307..3979 /label=BBa_K228000 stem_loop 3999..4042 /label=stem_loop misc_feature 3988..4067 /label=BBa_B0010 terminator 3988..4067 /label=BBa_B0010 stem_loop 4083..4102 /label=T7 TE misc_feature 4109..4109 /label=stop polyA_site 4103..4116 /label=polya misc_feature 4076..4116 /label=BBa_B0012 terminator 4076..4116 /label=BBa_B0012 misc_feature 1288..4116 /label=BBa_K228202 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagtca cacaggaaag tactagatga ccatgattac 1321 cgtgcactag aataccatta acattgctaa gaacgacttc tctgacatcg aactggctgc 1381 tatcccgttc aacactctgg ctgaccatta cggtgagcgt ttagctcgcg aacagttggc 1441 ccttgagcat gagtcttacg agatgggtga agcacgcttc cgcaagatgt ttgagcgtca 1501 acttaaagct ggtgaggttg cggataacgc tgccgccaag cctctcatca ctaccctact 1561 ccctaagatg attgcacgca tcaacgactg gtttgaggaa gtgaaagcta agcgcggcaa 1621 gcgcccgaca gccttccagt tcctgtagga aatcaagccg gaagccgtag cgtacatcac 1681 cattaagacc actctggctt gcctaaccag tgctgacaat acaaccgttc aggctgtagc 1741 aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc gtgaccttga 1801 agctaagcac ttcaagaaaa acgttgagga acaactcaac aagcgcgtag ggcacgtcta 1861 caagaaagca tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc tactcggtgg 1921 cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat gtaggagtac gctgcatcga 1981 gatgctcatt gagtcaaccg gaatggttag cttacaccgc caaaatgctg gcgtagtagg 2041 tcaagactct gagactatcg aactcgcacc tgaatacgct gaggctatcg caacccgtgc 2101 aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc gtagttcctc ctaagccgtg 2161 gactggcatt actggtggtg gctattgggc taacggtcgt cgtcctctgg cgctggtgcg 2221 tactcacagt aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg aggtgtacaa 2281 agcgattaac attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc tagcggtcgc 2341 caacgtaatc accaagtgga agcattgtcc ggtcgaggac atccctgcga ttgagcgtga 2401 agaactcccg atgaaaccgg aagacatcga catgaatcct gaggctctca ccgcgtggaa 2461 acgtgctgcc gctgctgtgt accgcaagga caaggctcgc aagtctcgcc gtatcagcct 2521 tgagttcatg cttgagcaag ccaataagtt tgctaaccat aaggccatct ggttccctta 2581 caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc aaggtaacga 2641 tatgaccaaa ggactgctta cgctggcgaa aggtaaacca atcggtaagg aaggttacta 2701 ctggctgaaa atccacggtg caaactgtgc gggtgtcgat aaggttccgt tccctgagcg 2761 catcaagttc attgaggaaa accacgagaa catcatggct tgcgctaagt ctccactgga 2821 gaacacttgg tgggctgagc aagattctcc gttctgcttc cttgcgttct gctttgagta 2881 cgctggggta cagcaccacg gcctgagcta taactgctcc cttccgctgg cgtttgacgg 2941 gtcttgctct ggcatccagc acttctccgc gatgctccga gatgaggtag gtggtcgcgc 3001 ggttaacttg cttcctagtg aaaccgttca ggacatctac gggattgttg ctaagaaagt 3061 caacgagatt ctacaagcag acgcaatcaa tgggaccgat aacgaagtag ttaccgtgac 3121 cgatgagaac actggtgaaa tctctgagaa agtcaagctg ggcactaagg cactggctgg 3181 tcaatggctg gcttacggtg ttactcgcag tgtgactaag cgttcagtca tgacgctggc 3241 ttacgggtcc aaagagttcg gcttccgtca acaagtgctg gaagatacca ttcagccagc 3301 tattgattcc ggcaagggtc tgatgttcac tcagccgaat caggctgctg gatacatggc 3361 taagctgatt tgggaatctg tgagcgtgac ggtggtagct gcggttgaag caatgaactg 3421 gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa gataagaaga ctggagagat 3481 tcttcgcaag cgttgcgctg tgcattgggt aactcctgat ggtttccctg tgtggcagga 3541 atacaagaag cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca 3601 gcctaccatt aacaccaaca aagatagcga gattgatgca cacaaacagg agtctggtat 3661 cgctcctaac tttgtacaca gccaagacgg tagccacctt cgtaagactg tagtgtgggc 3721 acacgagaag tacggaatcg aatcttttgc actgattcac gactccttcg gtaccattcc 3781 ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca catatgagtc 3841 ttgtgatgta ctggctgatt tctacgacca gttcgctgac cagttgcacg agtctcaatt 3901 ggacaaaatg ccagcacttc cggctaaagg taacttgaac ctccgtgaca tcttagagtc 3961 ggacttcgcg ttcgcataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 4021 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 4081 ctggctcacc ttcgggtggg cctttctgcg tttata //