BBa_K228211
type: ComponentDefinition
persistent identity: BBa K228211
display id: BBa_K228211
version: 1
was generated by: iGEM to SBOL conversion
description: AraC->rbs->T7ptag->Terminator
created: 2009-09-19T11:00:00Z
modified: 2015-05-08T01:11:33Z
mutable provenance: Built by joining different standardized biobricks together
owned by: james
owned by: myers
mutable description: This is a branch of the AND GATE built by Peking University 2009 iGEM Team. The addition of arabinose can induce the transcription of T7ptag. However, if the SupD tRNA is absent, T7ptag might not be translated into functional T7 polymerase normally because of some amber stop codon mutations in it. We also have constructed a few similar parts with various RBS, which would make the expression regulation of T7ptag gene more convenient by choosing different parts.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4413
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Guosheng Zhang
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0010
component: BBa_K228009
component: BBa_B0012
component: BBa_K228202
sequence annotation: BBa_K228009
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K228202
sequence: https://synbiohub.org/public/igem/BBa_K228211_sequence/1

BBa_B0010
type: Component
persistent identity: component2262368
display id: component2262368
version: 1
top level: BBa_K228211
definition: BBa_B0010
access: public

BBa_K228009
type: Component
persistent identity: component2262376
display id: component2262376
version: 1
top level: BBa_K228211
definition: BBa_K228009
access: public

BBa_B0012
type: Component
persistent identity: component2262370
display id: component2262370
version: 1
top level: BBa_K228211
definition: BBa_B0012
access: public

BBa_K228202
type: Component
persistent identity: component2262388
display id: component2262388
version: 1
top level: BBa_K228211
definition: BBa_K228202
access: public

BBa_K228009
type: SequenceAnnotation
persistent identity: annotation2262376
display id: annotation2262376
version: 1
top level: BBa_K228211
location: https://synbiohub.org/public/igem/BBa_K228211/annotation2262376/range2262376/1
component: BBa_K228009

https://synbiohub.org/public/igem/BBa_K228211/annotation2262376/range2262376/1
type: Range
persistent identity: range2262376
display id: range2262376
version: 1
top level: BBa_K228211
direction: direction/reverse
start: 1
end: 1279
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2262368
display id: annotation2262368
version: 1
top level: BBa_K228211
location: https://synbiohub.org/public/igem/BBa_K228211/annotation2262368/range2262368/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K228211/annotation2262368/range2262368/1
type: Range
persistent identity: range2262368
display id: range2262368
version: 1
top level: BBa_K228211
direction: direction/forward
start: 1013
end: 1092
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2262370
display id: annotation2262370
version: 1
top level: BBa_K228211
location: https://synbiohub.org/public/igem/BBa_K228211/annotation2262370/range2262370/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K228211/annotation2262370/range2262370/1
type: Range
persistent identity: range2262370
display id: range2262370
version: 1
top level: BBa_K228211
direction: direction/forward
start: 1101
end: 1141
orientation: inline

BBa_K228202
type: SequenceAnnotation
persistent identity: annotation2262388
display id: annotation2262388
version: 1
top level: BBa_K228211
location: https://synbiohub.org/public/igem/BBa_K228211/annotation2262388/range2262388/1
component: BBa_K228202

https://synbiohub.org/public/igem/BBa_K228211/annotation2262388/range2262388/1
type: Range
persistent identity: range2262388
display id: range2262388
version: 1
top level: BBa_K228211
direction: direction/reverse
start: 1288
end: 4116
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K228009
type: ComponentDefinition
persistent identity: BBa K228009
display id: BBa_K228009
version: 1
was generated by: iGEM to SBOL conversion
description: AraC protein(reversed sequence) and Pbad promoter
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:32Z
owned by: james
owned by: myers
mutable description: This part contains AraC protein generator(BBa_K228008) and Pbad promoter(BBa_I13453). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The maximum concentration of L-arabinose we tested is 10^-4 M.
star: false
creator: Gao Rencheng
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_K228008
component: BBa_I13453
sequence annotation: BBa_K228008
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K228009_sequence/1

BBa_K228008
type: Component
persistent identity: component2244233
display id: component2244233
version: 1
top level: BBa_K228009
definition: BBa_K228008
access: public

BBa_I13453
type: Component
persistent identity: component2244232
display id: component2244232
version: 1
top level: BBa_K228009
definition: BBa_I13453
access: public

BBa_I13453
type: Component
persistent identity: component2244234
display id: component2244234
version: 1
top level: BBa_K228009
definition: BBa_I13453
access: public

BBa_K228008
type: SequenceAnnotation
persistent identity: annotation2244233
display id: annotation2244233
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244233/range2244233/1
component: BBa_K228008

https://synbiohub.org/public/igem/BBa_K228009/annotation2244233/range2244233/1
type: Range
persistent identity: range2244233
display id: range2244233
version: 1
top level: BBa_K228009
direction: direction/reverse
start: 1
end: 1141
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2244232
display id: annotation2244232
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244232/range2244232/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K228009/annotation2244232/range2244232/1
type: Range
persistent identity: range2244232
display id: range2244232
version: 1
top level: BBa_K228009
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2244234
display id: annotation2244234
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244234/range2244234/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K228009/annotation2244234/range2244234/1
type: Range
persistent identity: range2244234
display id: range2244234
version: 1
top level: BBa_K228009
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

BBa_K228202
type: ComponentDefinition
persistent identity: BBa K228202
display id: BBa_K228202
version: 1
was generated by: iGEM to SBOL conversion
description: RBS->T7ptag->Terminator
created: 2009-09-15T11:00:00Z
modified: 2015-05-08T01:11:33Z
mutable provenance: Built from standardized parts
owned by: james
owned by: myers
mutable description: The part is RBS->T7ptag->Terminator. K228002 is added to the RBS BBa_B0032 to construct this part. We also have constructed a few similar parts with various RBS, which would make the expression regulation of T7ptag gene more convenient by choosing different parts.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4413
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Because the RBS is only about 10bp, so let the BBa_K228002 be the insert and the backbone with RBS be the vector would be more convenient.
star: false
creator: Guosheng Zhang
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0010
component: BBa_K228000
component: BBa_B0012
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_K228000
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K228202_sequence/1

BBa_B0010
type: Component
persistent identity: component2021245
display id: component2021245
version: 1
top level: BBa_K228202
definition: BBa_B0010
access: public

BBa_K228000
type: Component
persistent identity: component2021244
display id: component2021244
version: 1
top level: BBa_K228202
definition: BBa_K228000
access: public

BBa_B0012
type: Component
persistent identity: component2021247
display id: component2021247
version: 1
top level: BBa_K228202
definition: BBa_B0012
access: public

BBa_B0032
type: Component
persistent identity: component2021241
display id: component2021241
version: 1
top level: BBa_K228202
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2021241
display id: annotation2021241
version: 1
top level: BBa_K228202
location: https://synbiohub.org/public/igem/BBa_K228202/annotation2021241/range2021241/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K228202/annotation2021241/range2021241/1
type: Range
persistent identity: range2021241
display id: range2021241
version: 1
top level: BBa_K228202
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K228000
type: SequenceAnnotation
persistent identity: annotation2021244
display id: annotation2021244
version: 1
top level: BBa_K228202
location: https://synbiohub.org/public/igem/BBa_K228202/annotation2021244/range2021244/1
component: BBa_K228000

https://synbiohub.org/public/igem/BBa_K228202/annotation2021244/range2021244/1
type: Range
persistent identity: range2021244
display id: range2021244
version: 1
top level: BBa_K228202
direction: direction/reverse
start: 20
end: 2692
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2021245
display id: annotation2021245
version: 1
top level: BBa_K228202
location: https://synbiohub.org/public/igem/BBa_K228202/annotation2021245/range2021245/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K228202/annotation2021245/range2021245/1
type: Range
persistent identity: range2021245
display id: range2021245
version: 1
top level: BBa_K228202
direction: direction/forward
start: 2701
end: 2780
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2021247
display id: annotation2021247
version: 1
top level: BBa_K228202
location: https://synbiohub.org/public/igem/BBa_K228202/annotation2021247/range2021247/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K228202/annotation2021247/range2021247/1
type: Range
persistent identity: range2021247
display id: range2021247
version: 1
top level: BBa_K228202
direction: direction/forward
start: 2789
end: 2829
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_K228000
type: ComponentDefinition
persistent identity: BBa K228000
display id: BBa_K228000
version: 1
was generated by: iGEM to SBOL conversion
description: T7ptag(T7polymerase with amber mutation)
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: The T7ptag is standardlized from the plasmid kindly provided by Voigt Lab.
owned by: james
owned by: myers
mutable description: T7ptag is a coding sequence of T7 polymerase with 2 Amber mutations. The transcription of T7ptag gene can only lead to the generation of its mRNA, further translation into T7 RNA polymerase is blocked because of the amber mutation. Only in the presence of the Amber mutation suppressor BBa_K228001 SupD-tRNA, it can be translated into functional T7 polymerase. T7ptag is a component of the AND Gate constructed by the Voigt Lab(UCSF)
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K228000_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2017714
display id: annotation2017714
version: 1
top level: BBa_K228000
location: https://synbiohub.org/public/igem/BBa_K228000/annotation2017714/range2017714/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K228000/annotation2017714/range2017714/1
type: Range
persistent identity: range2017714
display id: range2017714
version: 1
top level: BBa_K228000
direction: direction/forward
start: 1
end: 2673
orientation: inline

BBa_K228008
type: ComponentDefinition
persistent identity: BBa K228008
display id: BBa_K228008
version: 1
was generated by: iGEM to SBOL conversion
description: AraC regulatory protein
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:32Z
owned by: james
owned by: myers
mutable description: This part could be connected to a protein coding sequence so that the expression could be induced by L-arabinose
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Gao Rencheng
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_I14033
sequence annotation: P(Cat)
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_B0034
sequence annotation: BBa_K228006
sequence annotation: conserved
sequence annotation: araC
sequence annotation: BBa_C0080
sequence annotation: LVA
sequence annotation: barcode
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K228008_sequence/1

BBa_I14033
type: SequenceAnnotation
persistent identity: annotation2020002
display id: annotation2020002
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020002/range2020002/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020002/range2020002/1
type: Range
persistent identity: range2020002
display id: range2020002
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 1
end: 38
orientation: inline

P(Cat)
type: SequenceAnnotation
persistent identity: annotation2019999
display id: annotation2019999
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2019999/range2019999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K228008/annotation2019999/range2019999/1
type: Range
persistent identity: range2019999
display id: range2019999
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1
end: 38
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2020000
display id: annotation2020000
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020000/range2020000/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228008/annotation2020000/range2020000/1
type: Range
persistent identity: range2020000
display id: range2020000
version: 1
top level: BBa_K228008
direction: direction/forward
start: 4
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2020001
display id: annotation2020001
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020001/range2020001/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228008/annotation2020001/range2020001/1
type: Range
persistent identity: range2020001
display id: range2020001
version: 1
top level: BBa_K228008
direction: direction/forward
start: 27
end: 32
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2020003
display id: annotation2020003
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020003/range2020003/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020003/range2020003/1
type: Range
persistent identity: range2020003
display id: range2020003
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 47
end: 58
orientation: inline

BBa_K228006
type: SequenceAnnotation
persistent identity: annotation2020015
display id: annotation2020015
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020015/range2020015/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020015/range2020015/1
type: Range
persistent identity: range2020015
display id: range2020015
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 47
end: 1141
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2020004
display id: annotation2020004
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020004/range2020004/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K228008/annotation2020004/range2020004/1
type: Range
persistent identity: range2020004
display id: range2020004
version: 1
top level: BBa_K228008
direction: direction/forward
start: 51
end: 54
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2020006
display id: annotation2020006
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020006/range2020006/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K228008/annotation2020006/range2020006/1
type: Range
persistent identity: range2020006
display id: range2020006
version: 1
top level: BBa_K228008
direction: direction/forward
start: 65
end: 940
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation2020005
display id: annotation2020005
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020005/range2020005/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020005/range2020005/1
type: Range
persistent identity: range2020005
display id: range2020005
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 65
end: 979
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2020007
display id: annotation2020007
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020007/range2020007/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K228008/annotation2020007/range2020007/1
type: Range
persistent identity: range2020007
display id: range2020007
version: 1
top level: BBa_K228008
direction: direction/forward
start: 941
end: 973
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation2020008
display id: annotation2020008
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020008/range2020008/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020008/range2020008/1
type: Range
persistent identity: range2020008
display id: range2020008
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 980
end: 1004
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2020009
display id: annotation2020009
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020009/range2020009/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020009/range2020009/1
type: Range
persistent identity: range2020009
display id: range2020009
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1013
end: 1092
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2020010
display id: annotation2020010
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020010/range2020010/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K228008/annotation2020010/range2020010/1
type: Range
persistent identity: range2020010
display id: range2020010
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1024
end: 1067
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2020011
display id: annotation2020011
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020011/range2020011/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020011/range2020011/1
type: Range
persistent identity: range2020011
display id: range2020011
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1101
end: 1141
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2020012
display id: annotation2020012
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020012/range2020012/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K228008/annotation2020012/range2020012/1
type: Range
persistent identity: range2020012
display id: range2020012
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1108
end: 1127
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2020013
display id: annotation2020013
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020013/range2020013/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_K228008/annotation2020013/range2020013/1
type: Range
persistent identity: range2020013
display id: range2020013
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1128
end: 1141
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2020014
display id: annotation2020014
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020014/range2020014/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K228008/annotation2020014/range2020014/1
type: Range
persistent identity: range2020014
display id: range2020014
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1134
end: 1134
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2020016
display id: annotation2020016
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020016/range2020016/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020016/range2020016/1
type: Range
persistent identity: range2020016
display id: range2020016
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

https://synbiohub.org/public/igem/BBa_K228211_sequence/1
type: Sequence
persistent identity: BBa K228211 sequence
display id: BBa_K228211_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagtcacacaggaaagtactagatgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228009_sequence/1
type: Sequence
persistent identity: BBa K228009 sequence
display id: BBa_K228009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228202_sequence/1
type: Sequence
persistent identity: BBa K228202 sequence
display id: BBa_K228202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228000_sequence/1
type: Sequence
persistent identity: BBa K228000 sequence
display id: BBa_K228000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228008_sequence/1
type: Sequence
persistent identity: BBa K228008 sequence
display id: BBa_K228008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z