LOCUS BBa_K228212 4114 bp DNA linear 26-Dec-2024 DEFINITION AraC->rbs->T7ptag->Terminator ACCESSION BBa_K228212 VERSION BBa_K228212.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 RBS 1288..1298 /label=RBS-4\Weaker RBS 1294..1297 /label=RBS misc_feature 1288..1298 /label=BBa_B0033 RBS 1288..1298 /label=BBa_B0033 CDS 1305..3977 /label=cds CDS 1305..3977 /label=BBa_K228000 stem_loop 3997..4040 /label=stem_loop misc_feature 3986..4065 /label=BBa_B0010 terminator 3986..4065 /label=BBa_B0010 stem_loop 4081..4100 /label=T7 TE misc_feature 4107..4107 /label=stop polyA_site 4101..4114 /label=polya misc_feature 4074..4114 /label=BBa_B0012 terminator 4074..4114 /label=BBa_B0012 misc_feature 1288..4114 /label=BBa_K228203 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagtca cacaggacta ctagatgacc atgattaccg 1321 tgcactagaa taccattaac attgctaaga acgacttctc tgacatcgaa ctggctgcta 1381 tcccgttcaa cactctggct gaccattacg gtgagcgttt agctcgcgaa cagttggccc 1441 ttgagcatga gtcttacgag atgggtgaag cacgcttccg caagatgttt gagcgtcaac 1501 ttaaagctgg tgaggttgcg gataacgctg ccgccaagcc tctcatcact accctactcc 1561 ctaagatgat tgcacgcatc aacgactggt ttgaggaagt gaaagctaag cgcggcaagc 1621 gcccgacagc cttccagttc ctgtaggaaa tcaagccgga agccgtagcg tacatcacca 1681 ttaagaccac tctggcttgc ctaaccagtg ctgacaatac aaccgttcag gctgtagcaa 1741 gcgcaatcgg tcgggccatt gaggacgagg ctcgcttcgg tcgtatccgt gaccttgaag 1801 ctaagcactt caagaaaaac gttgaggaac aactcaacaa gcgcgtaggg cacgtctaca 1861 agaaagcatt tatgcaagtt gtcgaggctg acatgctctc taagggtcta ctcggtggcg 1921 aggcgtggtc ttcgtggcat aaggaagact ctattcatgt aggagtacgc tgcatcgaga 1981 tgctcattga gtcaaccgga atggttagct tacaccgcca aaatgctggc gtagtaggtc 2041 aagactctga gactatcgaa ctcgcacctg aatacgctga ggctatcgca acccgtgcag 2101 gtgcgctggc tggcatctct ccgatgttcc aaccttgcgt agttcctcct aagccgtgga 2161 ctggcattac tggtggtggc tattgggcta acggtcgtcg tcctctggcg ctggtgcgta 2221 ctcacagtaa gaaagcactg atgcgctacg aagacgttta catgcctgag gtgtacaaag 2281 cgattaacat tgcgcaaaac accgcatgga aaatcaacaa gaaagtccta gcggtcgcca 2341 acgtaatcac caagtggaag cattgtccgg tcgaggacat ccctgcgatt gagcgtgaag 2401 aactcccgat gaaaccggaa gacatcgaca tgaatcctga ggctctcacc gcgtggaaac 2461 gtgctgccgc tgctgtgtac cgcaaggaca aggctcgcaa gtctcgccgt atcagccttg 2521 agttcatgct tgagcaagcc aataagtttg ctaaccataa ggccatctgg ttcccttaca 2581 acatggactg gcgcggtcgt gtttacgctg tgtcaatgtt caacccgcaa ggtaacgata 2641 tgaccaaagg actgcttacg ctggcgaaag gtaaaccaat cggtaaggaa ggttactact 2701 ggctgaaaat ccacggtgca aactgtgcgg gtgtcgataa ggttccgttc cctgagcgca 2761 tcaagttcat tgaggaaaac cacgagaaca tcatggcttg cgctaagtct ccactggaga 2821 acacttggtg ggctgagcaa gattctccgt tctgcttcct tgcgttctgc tttgagtacg 2881 ctggggtaca gcaccacggc ctgagctata actgctccct tccgctggcg tttgacgggt 2941 cttgctctgg catccagcac ttctccgcga tgctccgaga tgaggtaggt ggtcgcgcgg 3001 ttaacttgct tcctagtgaa accgttcagg acatctacgg gattgttgct aagaaagtca 3061 acgagattct acaagcagac gcaatcaatg ggaccgataa cgaagtagtt accgtgaccg 3121 atgagaacac tggtgaaatc tctgagaaag tcaagctggg cactaaggca ctggctggtc 3181 aatggctggc ttacggtgtt actcgcagtg tgactaagcg ttcagtcatg acgctggctt 3241 acgggtccaa agagttcggc ttccgtcaac aagtgctgga agataccatt cagccagcta 3301 ttgattccgg caagggtctg atgttcactc agccgaatca ggctgctgga tacatggcta 3361 agctgatttg ggaatctgtg agcgtgacgg tggtagctgc ggttgaagca atgaactggc 3421 ttaagtctgc tgctaagctg ctggctgctg aggtcaaaga taagaagact ggagagattc 3481 ttcgcaagcg ttgcgctgtg cattgggtaa ctcctgatgg tttccctgtg tggcaggaat 3541 acaagaagcc tattcagacg cgcttgaacc tgatgttcct cggtcagttc cgcttacagc 3601 ctaccattaa caccaacaaa gatagcgaga ttgatgcaca caaacaggag tctggtatcg 3661 ctcctaactt tgtacacagc caagacggta gccaccttcg taagactgta gtgtgggcac 3721 acgagaagta cggaatcgaa tcttttgcac tgattcacga ctccttcggt accattccgg 3781 ctgacgctgc gaacctgttc aaagcagtgc gcgaaactat ggttgacaca tatgagtctt 3841 gtgatgtact ggctgatttc tacgaccagt tcgctgacca gttgcacgag tctcaattgg 3901 acaaaatgcc agcacttccg gctaaaggta acttgaacct ccgtgacatc ttagagtcgg 3961 acttcgcgtt cgcataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 4021 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 4081 ggctcacctt cgggtgggcc tttctgcgtt tata //