##gff-version 3
##sequence-region BBa_K228212 1 4114
BBa_K228212	.	terminator	1101	1141	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228212	.	polyA_site	1128	1141	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228212	.	stem_loop	1108	1127	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228212	.	stop_codon	1134	1134	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228212	.	engineered_region	1101	1141	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228212	.	promoter	1	1279	.	+	0	ID=BBa_K228009;Name=BBa_K228009
BBa_K228212	.	promoter	1150	1279	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228212	.	engineered_region	1	1141	.	+	0	ID=BBa_K228008;Name=BBa_K228008;Parent=BBa_K228009
BBa_K228212	.	polyA_site	1128	1141	.	+	0	ID=annotation2020013;Name=polya;Parent=BBa_K228008
BBa_K228212	.	conserved_region	51	54	.	+	0	ID=annotation2020004;Name=conserved;Parent=BBa_K228008
BBa_K228212	.	promoter	27	32	.	+	0	ID=annotation2020001;Name=-10;Parent=BBa_K228008
BBa_K228212	.	engineered_region	1	38	.	+	0	ID=annotation2020002;Name=BBa_I14033;Parent=BBa_K228008
BBa_K228212	.	engineered_region	47	1141	.	+	0	ID=annotation2020015;Name=BBa_K228006;Parent=BBa_K228008
BBa_K228212	.	tag	941	973	.	+	0	ID=annotation2020007;Name=LVA;Parent=BBa_K228008
BBa_K228212	.	engineered_region	1013	1092	.	+	0	ID=annotation2020009;Name=BBa_B0010;Parent=BBa_K228008
BBa_K228212	.	engineered_region	1101	1141	.	+	0	ID=annotation2020011;Name=BBa_B0012;Parent=BBa_K228008
BBa_K228212	.	stop_codon	1134	1134	.	+	0	ID=annotation2020014;Name=stop;Parent=BBa_K228008
BBa_K228212	.	engineered_region	65	979	.	+	0	ID=annotation2020005;Name=BBa_C0080;Parent=BBa_K228008
BBa_K228212	.	engineered_region	1150	1279	.	+	0	ID=annotation2020016;Name=BBa_I13453;Parent=BBa_K228008
BBa_K228212	.	stem_loop	1108	1127	.	+	0	ID=annotation2020012;Name=T7 TE;Parent=BBa_K228008
BBa_K228212	.	engineered_region	47	58	.	+	0	ID=annotation2020003;Name=BBa_B0034;Parent=BBa_K228008
BBa_K228212	.	operator	1	38	.	+	0	ID=annotation2019999;Name=P(Cat);Parent=BBa_K228008
BBa_K228212	.	promoter	4	9	.	+	0	ID=annotation2020000;Name=-35;Parent=BBa_K228008
BBa_K228212	.	stem_loop	1024	1067	.	+	0	ID=annotation2020010;Name=stem_loop;Parent=BBa_K228008
BBa_K228212	.	CDS	65	940	.	+	0	ID=annotation2020006;Name=araC;Parent=BBa_K228008
BBa_K228212	.	engineered_region	980	1004	.	+	0	ID=annotation2020008;Name=barcode;Parent=BBa_K228008
BBa_K228212	.	promoter	1150	1279	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228212	.	engineered_region	1288	4114	.	+	0	ID=BBa_K228203;Name=BBa_K228203
BBa_K228212	.	terminator	4074	4114	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228203
BBa_K228212	.	polyA_site	4101	4114	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228212	.	stem_loop	4081	4100	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228212	.	stop_codon	4107	4107	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228212	.	engineered_region	4074	4114	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228212	.	terminator	3986	4065	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228203
BBa_K228212	.	stem_loop	3997	4040	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228212	.	engineered_region	3986	4065	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228212	.	CDS	1305	3977	.	+	0	ID=BBa_K228000;Name=BBa_K228000;Parent=BBa_K228203
BBa_K228212	.	CDS	1305	3977	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228212	.	ribosome_entry_site	1288	1298	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_K228203
BBa_K228212	.	engineered_region	1288	1298	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K228212	.	ribosome_entry_site	1294	1297	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K228212	.	ribosome_entry_site	1288	1298	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K228212	.	terminator	1013	1092	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228212	.	stem_loop	1024	1067	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228212	.	engineered_region	1013	1092	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K228212
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatc
agtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttc
acaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaa
ccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcct
cgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctg
tgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatcc
atcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagct
ccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatc
cgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaa
acccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatat
cacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacc
tttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgg
gcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgt
ttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatg
ccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccg
tttttttgggctagctactagagtcacacaggactactagatgaccatgattaccgtgcactagaataccattaacatt
gctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttag
ctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaact
taaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatc
aacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccgg
aagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagc
aagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaa
aacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgc
tctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcat
cgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagact
atcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaac
cttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgct
ggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacatt
gcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccgg
tcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcac
cgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatg
cttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacg
ctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaa
ggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaag
ttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagatt
ctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttcc
gctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggtt
aacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacg
caatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctggg
cactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggct
tacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtc
tgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagc
tgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagag
attcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattc
agacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattga
tgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtg
tgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcga
acctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagtt
cgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatc
ttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttata