LOCUS BBa_K228217 4115 bp DNA linear 22-Dec-2024 DEFINITION AraC->rbs->T7ptag->Terminator ACCESSION BBa_K228217 VERSION BBa_K228217.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 RBS 1288..1299 /label=BBa_J61127 CDS 1306..3978 /label=cds CDS 1306..3978 /label=BBa_K228000 stem_loop 3998..4041 /label=stem_loop misc_feature 3987..4066 /label=BBa_B0010 terminator 3987..4066 /label=BBa_B0010 stem_loop 4082..4101 /label=T7 TE misc_feature 4108..4108 /label=stop polyA_site 4102..4115 /label=polya misc_feature 4075..4115 /label=BBa_B0012 terminator 4075..4115 /label=BBa_B0012 misc_feature 1288..4115 /label=BBa_K228208 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagaaa gagtggaact actagatgac catgattacc 1321 gtgcactaga ataccattaa cattgctaag aacgacttct ctgacatcga actggctgct 1381 atcccgttca acactctggc tgaccattac ggtgagcgtt tagctcgcga acagttggcc 1441 cttgagcatg agtcttacga gatgggtgaa gcacgcttcc gcaagatgtt tgagcgtcaa 1501 cttaaagctg gtgaggttgc ggataacgct gccgccaagc ctctcatcac taccctactc 1561 cctaagatga ttgcacgcat caacgactgg tttgaggaag tgaaagctaa gcgcggcaag 1621 cgcccgacag ccttccagtt cctgtaggaa atcaagccgg aagccgtagc gtacatcacc 1681 attaagacca ctctggcttg cctaaccagt gctgacaata caaccgttca ggctgtagca 1741 agcgcaatcg gtcgggccat tgaggacgag gctcgcttcg gtcgtatccg tgaccttgaa 1801 gctaagcact tcaagaaaaa cgttgaggaa caactcaaca agcgcgtagg gcacgtctac 1861 aagaaagcat ttatgcaagt tgtcgaggct gacatgctct ctaagggtct actcggtggc 1921 gaggcgtggt cttcgtggca taaggaagac tctattcatg taggagtacg ctgcatcgag 1981 atgctcattg agtcaaccgg aatggttagc ttacaccgcc aaaatgctgg cgtagtaggt 2041 caagactctg agactatcga actcgcacct gaatacgctg aggctatcgc aacccgtgca 2101 ggtgcgctgg ctggcatctc tccgatgttc caaccttgcg tagttcctcc taagccgtgg 2161 actggcatta ctggtggtgg ctattgggct aacggtcgtc gtcctctggc gctggtgcgt 2221 actcacagta agaaagcact gatgcgctac gaagacgttt acatgcctga ggtgtacaaa 2281 gcgattaaca ttgcgcaaaa caccgcatgg aaaatcaaca agaaagtcct agcggtcgcc 2341 aacgtaatca ccaagtggaa gcattgtccg gtcgaggaca tccctgcgat tgagcgtgaa 2401 gaactcccga tgaaaccgga agacatcgac atgaatcctg aggctctcac cgcgtggaaa 2461 cgtgctgccg ctgctgtgta ccgcaaggac aaggctcgca agtctcgccg tatcagcctt 2521 gagttcatgc ttgagcaagc caataagttt gctaaccata aggccatctg gttcccttac 2581 aacatggact ggcgcggtcg tgtttacgct gtgtcaatgt tcaacccgca aggtaacgat 2641 atgaccaaag gactgcttac gctggcgaaa ggtaaaccaa tcggtaagga aggttactac 2701 tggctgaaaa tccacggtgc aaactgtgcg ggtgtcgata aggttccgtt ccctgagcgc 2761 atcaagttca ttgaggaaaa ccacgagaac atcatggctt gcgctaagtc tccactggag 2821 aacacttggt gggctgagca agattctccg ttctgcttcc ttgcgttctg ctttgagtac 2881 gctggggtac agcaccacgg cctgagctat aactgctccc ttccgctggc gtttgacggg 2941 tcttgctctg gcatccagca cttctccgcg atgctccgag atgaggtagg tggtcgcgcg 3001 gttaacttgc ttcctagtga aaccgttcag gacatctacg ggattgttgc taagaaagtc 3061 aacgagattc tacaagcaga cgcaatcaat gggaccgata acgaagtagt taccgtgacc 3121 gatgagaaca ctggtgaaat ctctgagaaa gtcaagctgg gcactaaggc actggctggt 3181 caatggctgg cttacggtgt tactcgcagt gtgactaagc gttcagtcat gacgctggct 3241 tacgggtcca aagagttcgg cttccgtcaa caagtgctgg aagataccat tcagccagct 3301 attgattccg gcaagggtct gatgttcact cagccgaatc aggctgctgg atacatggct 3361 aagctgattt gggaatctgt gagcgtgacg gtggtagctg cggttgaagc aatgaactgg 3421 cttaagtctg ctgctaagct gctggctgct gaggtcaaag ataagaagac tggagagatt 3481 cttcgcaagc gttgcgctgt gcattgggta actcctgatg gtttccctgt gtggcaggaa 3541 tacaagaagc ctattcagac gcgcttgaac ctgatgttcc tcggtcagtt ccgcttacag 3601 cctaccatta acaccaacaa agatagcgag attgatgcac acaaacagga gtctggtatc 3661 gctcctaact ttgtacacag ccaagacggt agccaccttc gtaagactgt agtgtgggca 3721 cacgagaagt acggaatcga atcttttgca ctgattcacg actccttcgg taccattccg 3781 gctgacgctg cgaacctgtt caaagcagtg cgcgaaacta tggttgacac atatgagtct 3841 tgtgatgtac tggctgattt ctacgaccag ttcgctgacc agttgcacga gtctcaattg 3901 gacaaaatgc cagcacttcc ggctaaaggt aacttgaacc tccgtgacat cttagagtcg 3961 gacttcgcgt tcgcataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 4021 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 4081 tggctcacct tcgggtgggc ctttctgcgt ttata //