LOCUS BBa_K228219 3882 bp DNA linear 01-Jun-2024 DEFINITION LuxR->rbs->T7ptag->Terminator ACCESSION BBa_K228219 VERSION BBa_K228219.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 misc_feature 1059..1062 /label=conserved RBS 1053..1066 /label=BBa_B0031 CDS 1073..3745 /label=cds CDS 1073..3745 /label=BBa_K228000 stem_loop 3765..3808 /label=stem_loop misc_feature 3754..3833 /label=BBa_B0010 terminator 3754..3833 /label=BBa_B0010 stem_loop 3849..3868 /label=T7 TE misc_feature 3875..3875 /label=stop polyA_site 3869..3882 /label=polya misc_feature 3842..3882 /label=BBa_B0012 terminator 3842..3882 /label=BBa_B0012 misc_feature 1053..3882 /label=BBa_K228201 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agtcacacag gaaacctact agatgaccat 1081 gattaccgtg cactagaata ccattaacat tgctaagaac gacttctctg acatcgaact 1141 ggctgctatc ccgttcaaca ctctggctga ccattacggt gagcgtttag ctcgcgaaca 1201 gttggccctt gagcatgagt cttacgagat gggtgaagca cgcttccgca agatgtttga 1261 gcgtcaactt aaagctggtg aggttgcgga taacgctgcc gccaagcctc tcatcactac 1321 cctactccct aagatgattg cacgcatcaa cgactggttt gaggaagtga aagctaagcg 1381 cggcaagcgc ccgacagcct tccagttcct gtaggaaatc aagccggaag ccgtagcgta 1441 catcaccatt aagaccactc tggcttgcct aaccagtgct gacaatacaa ccgttcaggc 1501 tgtagcaagc gcaatcggtc gggccattga ggacgaggct cgcttcggtc gtatccgtga 1561 ccttgaagct aagcacttca agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca 1621 cgtctacaag aaagcattta tgcaagttgt cgaggctgac atgctctcta agggtctact 1681 cggtggcgag gcgtggtctt cgtggcataa ggaagactct attcatgtag gagtacgctg 1741 catcgagatg ctcattgagt caaccggaat ggttagctta caccgccaaa atgctggcgt 1801 agtaggtcaa gactctgaga ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac 1861 ccgtgcaggt gcgctggctg gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa 1921 gccgtggact ggcattactg gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct 1981 ggtgcgtact cacagtaaga aagcactgat gcgctacgaa gacgtttaca tgcctgaggt 2041 gtacaaagcg attaacattg cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc 2101 ggtcgccaac gtaatcacca agtggaagca ttgtccggtc gaggacatcc ctgcgattga 2161 gcgtgaagaa ctcccgatga aaccggaaga catcgacatg aatcctgagg ctctcaccgc 2221 gtggaaacgt gctgccgctg ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat 2281 cagccttgag ttcatgcttg agcaagccaa taagtttgct aaccataagg ccatctggtt 2341 cccttacaac atggactggc gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg 2401 taacgatatg accaaaggac tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg 2461 ttactactgg ctgaaaatcc acggtgcaaa ctgtgcgggt gtcgataagg ttccgttccc 2521 tgagcgcatc aagttcattg aggaaaacca cgagaacatc atggcttgcg ctaagtctcc 2581 actggagaac acttggtggg ctgagcaaga ttctccgttc tgcttccttg cgttctgctt 2641 tgagtacgct ggggtacagc accacggcct gagctataac tgctcccttc cgctggcgtt 2701 tgacgggtct tgctctggca tccagcactt ctccgcgatg ctccgagatg aggtaggtgg 2761 tcgcgcggtt aacttgcttc ctagtgaaac cgttcaggac atctacggga ttgttgctaa 2821 gaaagtcaac gagattctac aagcagacgc aatcaatggg accgataacg aagtagttac 2881 cgtgaccgat gagaacactg gtgaaatctc tgagaaagtc aagctgggca ctaaggcact 2941 ggctggtcaa tggctggctt acggtgttac tcgcagtgtg actaagcgtt cagtcatgac 3001 gctggcttac gggtccaaag agttcggctt ccgtcaacaa gtgctggaag ataccattca 3061 gccagctatt gattccggca agggtctgat gttcactcag ccgaatcagg ctgctggata 3121 catggctaag ctgatttggg aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat 3181 gaactggctt aagtctgctg ctaagctgct ggctgctgag gtcaaagata agaagactgg 3241 agagattctt cgcaagcgtt gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg 3301 gcaggaatac aagaagccta ttcagacgcg cttgaacctg atgttcctcg gtcagttccg 3361 cttacagcct accattaaca ccaacaaaga tagcgagatt gatgcacaca aacaggagtc 3421 tggtatcgct cctaactttg tacacagcca agacggtagc caccttcgta agactgtagt 3481 gtgggcacac gagaagtacg gaatcgaatc ttttgcactg attcacgact ccttcggtac 3541 cattccggct gacgctgcga acctgttcaa agcagtgcgc gaaactatgg ttgacacata 3601 tgagtcttgt gatgtactgg ctgatttcta cgaccagttc gctgaccagt tgcacgagtc 3661 tcaattggac aaaatgccag cacttccggc taaaggtaac ttgaacctcc gtgacatctt 3721 agagtcggac ttcgcgttcg cataatacta gagccaggca tcaaataaaa cgaaaggctc 3781 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 3841 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //