##gff-version 3
##sequence-region BBa_K228219 1 3882
BBa_K228219	.	engineered_region	1053	3882	.	+	0	ID=BBa_K228201;Name=BBa_K228201
BBa_K228219	.	terminator	3754	3833	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228201
BBa_K228219	.	engineered_region	3754	3833	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228219	.	stem_loop	3765	3808	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228219	.	ribosome_entry_site	1053	1066	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K228201
BBa_K228219	.	conserved_region	1059	1062	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K228219	.	terminator	3842	3882	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228201
BBa_K228219	.	polyA_site	3869	3882	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228219	.	engineered_region	3842	3882	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228219	.	stop_codon	3875	3875	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228219	.	stem_loop	3849	3868	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228219	.	CDS	1073	3745	.	+	0	ID=BBa_K228000;Name=BBa_K228000;Parent=BBa_K228201
BBa_K228219	.	CDS	1073	3745	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228219	.	engineered_region	1	1044	.	+	0	ID=BBa_J09855;Name=BBa_J09855
BBa_K228219	.	engineered_region	46	981	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_K228219	.	CDS	64	819	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K228219	.	engineered_region	64	65	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K228219	.	engineered_region	64	819	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K228219	.	engineered_tag	820	844	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K228219	.	point_mutation	237	237	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K228219	.	point_mutation	555	555	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K228219	.	CDS	64	813	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K228219	.	terminator	941	981	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K228219	.	polyA_site	968	981	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228219	.	engineered_region	941	981	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228219	.	stop_codon	974	974	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228219	.	stem_loop	948	967	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228219	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K228219	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K228219	.	terminator	853	932	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K228219	.	engineered_region	853	932	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228219	.	stem_loop	864	907	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228219	.	promoter	990	1044	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_K228219	.	non_covalent_binding_site	990	1009	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K228219	.	promoter	1031	1036	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K228219	.	promoter	1009	1014	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K228219	.	start_codon	1042	1042	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K228219	.	engineered_region	990	1044	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K228219	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_K228219	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K228219	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K228219	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
>BBa_K228219
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtt
tgttatagtcgaataaatactagagtcacacaggaaacctactagatgaccatgattaccgtgcactagaataccatta
acattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcg
tttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgt
caacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcac
gcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaa
gccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggct
gtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttca
agaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctga
catgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgc
tgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctg
agactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgtt
ccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctg
gcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgatta
acattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattg
tccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggct
ctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagt
tcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgt
ttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatc
ggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgca
tcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagca
agattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcc
cttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcg
cggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagc
agacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaag
ctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgc
tggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaa
gggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtg
gtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactg
gagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcc
tattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgag
attgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactg
tagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgc
tgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgac
cagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtg
acatcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttata