##gff-version 3
##sequence-region BBa_K228220 1 3881
BBa_K228220	.	engineered_region	1053	3881	.	+	0	ID=BBa_K228202;Name=BBa_K228202
BBa_K228220	.	terminator	3841	3881	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228202
BBa_K228220	.	polyA_site	3868	3881	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228220	.	engineered_region	3841	3881	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228220	.	stem_loop	3848	3867	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228220	.	stop_codon	3874	3874	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228220	.	ribosome_entry_site	1053	1065	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K228202
BBa_K228220	.	engineered_region	1053	1065	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K228220	.	ribosome_entry_site	1053	1065	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K228220	.	ribosome_entry_site	1059	1062	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K228220	.	CDS	1072	3744	.	+	0	ID=BBa_K228000;Name=BBa_K228000;Parent=BBa_K228202
BBa_K228220	.	CDS	1072	3744	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228220	.	terminator	3753	3832	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228202
BBa_K228220	.	stem_loop	3764	3807	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228220	.	engineered_region	3753	3832	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228220	.	engineered_region	1	1044	.	+	0	ID=BBa_J09855;Name=BBa_J09855
BBa_K228220	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_K228220	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K228220	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K228220	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K228220	.	promoter	990	1044	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_K228220	.	engineered_region	990	1044	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K228220	.	promoter	1009	1014	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K228220	.	promoter	1031	1036	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K228220	.	start_codon	1042	1042	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K228220	.	non_covalent_binding_site	990	1009	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K228220	.	engineered_region	46	981	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_K228220	.	terminator	853	932	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K228220	.	stem_loop	864	907	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228220	.	engineered_region	853	932	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228220	.	terminator	941	981	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K228220	.	polyA_site	968	981	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228220	.	engineered_region	941	981	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228220	.	stem_loop	948	967	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228220	.	stop_codon	974	974	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228220	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K228220	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K228220	.	CDS	64	819	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K228220	.	CDS	64	813	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K228220	.	engineered_tag	820	844	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K228220	.	engineered_region	64	819	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K228220	.	point_mutation	237	237	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K228220	.	engineered_region	64	65	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K228220	.	point_mutation	555	555	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
>BBa_K228220
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtt
tgttatagtcgaataaatactagagtcacacaggaaagtactagatgaccatgattaccgtgcactagaataccattaa
cattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgt
ttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtc
aacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacg
catcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaag
ccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctg
tagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaa
gaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgac
atgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgct
gcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctga
gactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttc
caaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctgg
cgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaa
cattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgt
ccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctc
tcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagtt
catgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtt
tacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcg
gtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcat
caagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaa
gattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctccc
ttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgc
ggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagca
gacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagc
tgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgct
ggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaag
ggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtgg
tagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactgg
agagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcct
attcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgaga
ttgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgt
agtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgct
gcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgacc
agttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtga
catcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata