LOCUS BBa_K228227 3891 bp DNA linear 22-Dec-2024 DEFINITION Sal->rbs->T7ptag->Terminator ACCESSION BBa_K228227 VERSION BBa_K228227.1 FEATURES Location/Qualifiers CDS 1..903 /label=NahR promoter 904..993 /label=Pr promoter promoter 982..1050 /label=pSal promoter 1..1052 /label=BBa_K228004 RBS 1061..1075 /label=RBS-1\Strong RBS 1068..1072 /label=RBS misc_feature 1061..1075 /label=BBa_B0030 RBS 1061..1075 /label=BBa_B0030 CDS 1082..3754 /label=cds CDS 1082..3754 /label=BBa_K228000 stem_loop 3774..3817 /label=stem_loop misc_feature 3763..3842 /label=BBa_B0010 terminator 3763..3842 /label=BBa_B0010 stem_loop 3858..3877 /label=T7 TE misc_feature 3884..3884 /label=stop polyA_site 3878..3891 /label=polya misc_feature 3851..3891 /label=BBa_B0012 terminator 3851..3891 /label=BBa_B0012 ORIGIN 1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg 61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa 121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag 181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat 241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc 301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt 361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa 421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa 481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg 541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc 601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt 661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg 721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt 781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg 841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc 901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga 961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca 1021 agtcatcaat aaagccatca cgagtaccat agtactagag attaaagagg agaaatacta 1081 gatgaccatg attaccgtgc actagaatac cattaacatt gctaagaacg acttctctga 1141 catcgaactg gctgctatcc cgttcaacac tctggctgac cattacggtg agcgtttagc 1201 tcgcgaacag ttggcccttg agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa 1261 gatgtttgag cgtcaactta aagctggtga ggttgcggat aacgctgccg ccaagcctct 1321 catcactacc ctactcccta agatgattgc acgcatcaac gactggtttg aggaagtgaa 1381 agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg taggaaatca agccggaagc 1441 cgtagcgtac atcaccatta agaccactct ggcttgccta accagtgctg acaatacaac 1501 cgttcaggct gtagcaagcg caatcggtcg ggccattgag gacgaggctc gcttcggtcg 1561 tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg 1621 cgtagggcac gtctacaaga aagcatttat gcaagttgtc gaggctgaca tgctctctaa 1681 gggtctactc ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg 1741 agtacgctgc atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa 1801 tgctggcgta gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc 1861 tatcgcaacc cgtgcaggtg cgctggctgg catctctccg atgttccaac cttgcgtagt 1921 tcctcctaag ccgtggactg gcattactgg tggtggctat tgggctaacg gtcgtcgtcc 1981 tctggcgctg gtgcgtactc acagtaagaa agcactgatg cgctacgaag acgtttacat 2041 gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa 2101 agtcctagcg gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg aggacatccc 2161 tgcgattgag cgtgaagaac tcccgatgaa accggaagac atcgacatga atcctgaggc 2221 tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc 2281 tcgccgtatc agccttgagt tcatgcttga gcaagccaat aagtttgcta accataaggc 2341 catctggttc ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa 2401 cccgcaaggt aacgatatga ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg 2461 taaggaaggt tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt 2521 tccgttccct gagcgcatca agttcattga ggaaaaccac gagaacatca tggcttgcgc 2581 taagtctcca ctggagaaca cttggtgggc tgagcaagat tctccgttct gcttccttgc 2641 gttctgcttt gagtacgctg gggtacagca ccacggcctg agctataact gctcccttcc 2701 gctggcgttt gacgggtctt gctctggcat ccagcacttc tccgcgatgc tccgagatga 2761 ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca tctacgggat 2821 tgttgctaag aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga 2881 agtagttacc gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac 2941 taaggcactg gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc 3001 agtcatgacg ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga 3061 taccattcag ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc 3121 tgctggatac atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt 3181 tgaagcaatg aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa 3241 gaagactgga gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt 3301 ccctgtgtgg caggaataca agaagcctat tcagacgcgc ttgaacctga tgttcctcgg 3361 tcagttccgc ttacagccta ccattaacac caacaaagat agcgagattg atgcacacaa 3421 acaggagtct ggtatcgctc ctaactttgt acacagccaa gacggtagcc accttcgtaa 3481 gactgtagtg tgggcacacg agaagtacgg aatcgaatct tttgcactga ttcacgactc 3541 cttcggtacc attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt 3601 tgacacatat gagtcttgtg atgtactggc tgatttctac gaccagttcg ctgaccagtt 3661 gcacgagtct caattggaca aaatgccagc acttccggct aaaggtaact tgaacctccg 3721 tgacatctta gagtcggact tcgcgttcgc ataatactag agccaggcat caaataaaac 3781 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 3841 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //