##gff-version 3
##sequence-region BBa_K228227 1 3891
BBa_K228227	.	ribosome_entry_site	1061	1075	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K228227	.	engineered_region	1061	1075	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K228227	.	ribosome_entry_site	1068	1072	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K228227	.	ribosome_entry_site	1061	1075	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K228227	.	promoter	1	1052	.	+	0	ID=BBa_K228004;Name=BBa_K228004
BBa_K228227	.	promoter	982	1050	.	+	0	ID=annotation2075287;Name=pSal;Parent=BBa_K228004
BBa_K228227	.	CDS	1	903	.	+	0	ID=annotation2075285;Name=NahR;Parent=BBa_K228004
BBa_K228227	.	promoter	904	993	.	+	0	ID=annotation2075286;Name=Pr promoter;Parent=BBa_K228004
BBa_K228227	.	terminator	3763	3842	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228227	.	engineered_region	3763	3842	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228227	.	stem_loop	3774	3817	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228227	.	terminator	3851	3891	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228227	.	stem_loop	3858	3877	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228227	.	engineered_region	3851	3891	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228227	.	stop_codon	3884	3884	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228227	.	polyA_site	3878	3891	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228227	.	CDS	1082	3754	.	+	0	ID=BBa_K228000;Name=BBa_K228000
BBa_K228227	.	CDS	1082	3754	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
>BBa_K228227
tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaac
atgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggcta
aacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacg
gatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacg
tggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagt
gattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgtt
ctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcaca
tccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggat
cgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcata
gggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattg
ctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacacca
ccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatat
tcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatc
aataaagccatcacgagtaccatagtactagagattaaagaggagaaatactagatgaccatgattaccgtgcactaga
ataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccatta
cggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatg
tttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaaga
tgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgta
ggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaacc
gttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagcta
agcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgt
cgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgta
ggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtc
aagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctc
tccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgt
cgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtaca
aagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtg
gaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaat
cctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatca
gccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcg
cggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggt
aaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttcc
ctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtg
ggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctat
aactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtag
gtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagat
tctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgag
aaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcag
tcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattga
ttccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagc
gtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagata
agaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaata
caagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaa
gatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttc
gtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattcc
ggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgat
ttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttga
acctccgtgacatcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttata