LOCUS BBa_K228228 3890 bp DNA linear 22-Dec-2024 DEFINITION Sal->rbs->T7ptag->Terminator ACCESSION BBa_K228228 VERSION BBa_K228228.1 FEATURES Location/Qualifiers CDS 1..903 /label=NahR promoter 904..993 /label=Pr promoter promoter 982..1050 /label=pSal promoter 1..1052 /label=BBa_K228004 misc_feature 1067..1070 /label=conserved RBS 1061..1074 /label=BBa_B0031 CDS 1081..3753 /label=cds CDS 1081..3753 /label=BBa_K228000 stem_loop 3773..3816 /label=stem_loop misc_feature 3762..3841 /label=BBa_B0010 terminator 3762..3841 /label=BBa_B0010 stem_loop 3857..3876 /label=T7 TE misc_feature 3883..3883 /label=stop polyA_site 3877..3890 /label=polya misc_feature 3850..3890 /label=BBa_B0012 terminator 3850..3890 /label=BBa_B0012 ORIGIN 1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg 61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa 121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag 181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat 241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc 301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt 361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa 421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa 481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg 541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc 601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt 661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg 721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt 781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg 841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc 901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga 961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca 1021 agtcatcaat aaagccatca cgagtaccat agtactagag tcacacagga aacctactag 1081 atgaccatga ttaccgtgca ctagaatacc attaacattg ctaagaacga cttctctgac 1141 atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 1201 cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 1261 atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 1321 atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 1381 gctaagcgcg gcaagcgccc gacagccttc cagttcctgt aggaaatcaa gccggaagcc 1441 gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgctga caatacaacc 1501 gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 1561 atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 1621 gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 1681 ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 1741 gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 1801 gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 1861 atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 1921 cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 1981 ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 2041 cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 2101 gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 2161 gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 2221 ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct 2281 cgccgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 2341 atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 2401 ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 2461 aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 2521 ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 2581 aagtctccac tggagaacac ttggtgggct gagcaagatt ctccgttctg cttccttgcg 2641 ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 2701 ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 2761 gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 2821 gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 2881 gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 2941 aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca 3001 gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat 3061 accattcagc cagctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 3121 gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 3181 gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 3241 aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 3301 cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 3361 cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 3421 caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 3481 actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 3541 ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 3601 gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 3661 cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 3721 gacatcttag agtcggactt cgcgttcgca taatactaga gccaggcatc aaataaaacg 3781 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 3841 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //