LOCUS BBa_K228229 3889 bp DNA linear 26-Dec-2024 DEFINITION Sal->rbs->T7ptag->Terminator ACCESSION BBa_K228229 VERSION BBa_K228229.1 FEATURES Location/Qualifiers CDS 1..903 /label=NahR promoter 904..993 /label=Pr promoter promoter 982..1050 /label=pSal promoter 1..1052 /label=BBa_K228004 RBS 1061..1073 /label=RBS-3\Weak RBS 1067..1070 /label=RBS misc_feature 1061..1073 /label=BBa_B0032 RBS 1061..1073 /label=BBa_B0032 CDS 1080..3752 /label=cds CDS 1080..3752 /label=BBa_K228000 stem_loop 3772..3815 /label=stem_loop misc_feature 3761..3840 /label=BBa_B0010 terminator 3761..3840 /label=BBa_B0010 stem_loop 3856..3875 /label=T7 TE misc_feature 3882..3882 /label=stop polyA_site 3876..3889 /label=polya misc_feature 3849..3889 /label=BBa_B0012 terminator 3849..3889 /label=BBa_B0012 ORIGIN 1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg 61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa 121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag 181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat 241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc 301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt 361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa 421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa 481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg 541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc 601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt 661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg 721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt 781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg 841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc 901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga 961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca 1021 agtcatcaat aaagccatca cgagtaccat agtactagag tcacacagga aagtactaga 1081 tgaccatgat taccgtgcac tagaatacca ttaacattgc taagaacgac ttctctgaca 1141 tcgaactggc tgctatcccg ttcaacactc tggctgacca ttacggtgag cgtttagctc 1201 gcgaacagtt ggcccttgag catgagtctt acgagatggg tgaagcacgc ttccgcaaga 1261 tgtttgagcg tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc aagcctctca 1321 tcactaccct actccctaag atgattgcac gcatcaacga ctggtttgag gaagtgaaag 1381 ctaagcgcgg caagcgcccg acagccttcc agttcctgta ggaaatcaag ccggaagccg 1441 tagcgtacat caccattaag accactctgg cttgcctaac cagtgctgac aatacaaccg 1501 ttcaggctgt agcaagcgca atcggtcggg ccattgagga cgaggctcgc ttcggtcgta 1561 tccgtgacct tgaagctaag cacttcaaga aaaacgttga ggaacaactc aacaagcgcg 1621 tagggcacgt ctacaagaaa gcatttatgc aagttgtcga ggctgacatg ctctctaagg 1681 gtctactcgg tggcgaggcg tggtcttcgt ggcataagga agactctatt catgtaggag 1741 tacgctgcat cgagatgctc attgagtcaa ccggaatggt tagcttacac cgccaaaatg 1801 ctggcgtagt aggtcaagac tctgagacta tcgaactcgc acctgaatac gctgaggcta 1861 tcgcaacccg tgcaggtgcg ctggctggca tctctccgat gttccaacct tgcgtagttc 1921 ctcctaagcc gtggactggc attactggtg gtggctattg ggctaacggt cgtcgtcctc 1981 tggcgctggt gcgtactcac agtaagaaag cactgatgcg ctacgaagac gtttacatgc 2041 ctgaggtgta caaagcgatt aacattgcgc aaaacaccgc atggaaaatc aacaagaaag 2101 tcctagcggt cgccaacgta atcaccaagt ggaagcattg tccggtcgag gacatccctg 2161 cgattgagcg tgaagaactc ccgatgaaac cggaagacat cgacatgaat cctgaggctc 2221 tcaccgcgtg gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc 2281 gccgtatcag ccttgagttc atgcttgagc aagccaataa gtttgctaac cataaggcca 2341 tctggttccc ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc 2401 cgcaaggtaa cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta 2461 aggaaggtta ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc 2521 cgttccctga gcgcatcaag ttcattgagg aaaaccacga gaacatcatg gcttgcgcta 2581 agtctccact ggagaacact tggtgggctg agcaagattc tccgttctgc ttccttgcgt 2641 tctgctttga gtacgctggg gtacagcacc acggcctgag ctataactgc tcccttccgc 2701 tggcgtttga cgggtcttgc tctggcatcc agcacttctc cgcgatgctc cgagatgagg 2761 taggtggtcg cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc tacgggattg 2821 ttgctaagaa agtcaacgag attctacaag cagacgcaat caatgggacc gataacgaag 2881 tagttaccgt gaccgatgag aacactggtg aaatctctga gaaagtcaag ctgggcacta 2941 aggcactggc tggtcaatgg ctggcttacg gtgttactcg cagtgtgact aagcgttcag 3001 tcatgacgct ggcttacggg tccaaagagt tcggcttccg tcaacaagtg ctggaagata 3061 ccattcagcc agctattgat tccggcaagg gtctgatgtt cactcagccg aatcaggctg 3121 ctggatacat ggctaagctg atttgggaat ctgtgagcgt gacggtggta gctgcggttg 3181 aagcaatgaa ctggcttaag tctgctgcta agctgctggc tgctgaggtc aaagataaga 3241 agactggaga gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc 3301 ctgtgtggca ggaatacaag aagcctattc agacgcgctt gaacctgatg ttcctcggtc 3361 agttccgctt acagcctacc attaacacca acaaagatag cgagattgat gcacacaaac 3421 aggagtctgg tatcgctcct aactttgtac acagccaaga cggtagccac cttcgtaaga 3481 ctgtagtgtg ggcacacgag aagtacggaa tcgaatcttt tgcactgatt cacgactcct 3541 tcggtaccat tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg 3601 acacatatga gtcttgtgat gtactggctg atttctacga ccagttcgct gaccagttgc 3661 acgagtctca attggacaaa atgccagcac ttccggctaa aggtaacttg aacctccgtg 3721 acatcttaga gtcggacttc gcgttcgcat aatactagag ccaggcatca aataaaacga 3781 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 3841 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //