LOCUS BBa_K228230 3887 bp DNA linear 21-May-2024 DEFINITION Sal->rbs->T7ptag->Terminator ACCESSION BBa_K228230 VERSION BBa_K228230.1 FEATURES Location/Qualifiers CDS 1..903 /label=NahR promoter 904..993 /label=Pr promoter promoter 982..1050 /label=pSal promoter 1..1052 /label=BBa_K228004 RBS 1061..1071 /label=RBS-4\Weaker RBS 1067..1070 /label=RBS misc_feature 1061..1071 /label=BBa_B0033 RBS 1061..1071 /label=BBa_B0033 CDS 1078..3750 /label=cds CDS 1078..3750 /label=BBa_K228000 stem_loop 3770..3813 /label=stem_loop misc_feature 3759..3838 /label=BBa_B0010 terminator 3759..3838 /label=BBa_B0010 stem_loop 3854..3873 /label=T7 TE misc_feature 3880..3880 /label=stop polyA_site 3874..3887 /label=polya misc_feature 3847..3887 /label=BBa_B0012 terminator 3847..3887 /label=BBa_B0012 ORIGIN 1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg 61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa 121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag 181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat 241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc 301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt 361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa 421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa 481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg 541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc 601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt 661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg 721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt 781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg 841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc 901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga 961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca 1021 agtcatcaat aaagccatca cgagtaccat agtactagag tcacacagga ctactagatg 1081 accatgatta ccgtgcacta gaataccatt aacattgcta agaacgactt ctctgacatc 1141 gaactggctg ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc 1201 gaacagttgg cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg 1261 tttgagcgtc aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc 1321 actaccctac tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct 1381 aagcgcggca agcgcccgac agccttccag ttcctgtagg aaatcaagcc ggaagccgta 1441 gcgtacatca ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt 1501 caggctgtag caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc 1561 cgtgaccttg aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta 1621 gggcacgtct acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt 1681 ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta 1741 cgctgcatcg agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct 1801 ggcgtagtag gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc 1861 gcaacccgtg caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct 1921 cctaagccgt ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg 1981 gcgctggtgc gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct 2041 gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc 2101 ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg 2161 attgagcgtg aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc 2221 accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc 2281 cgtatcagcc ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc 2341 tggttccctt acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg 2401 caaggtaacg atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag 2461 gaaggttact actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg 2521 ttccctgagc gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag 2581 tctccactgg agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc 2641 tgctttgagt acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg 2701 gcgtttgacg ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta 2761 ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt 2821 gctaagaaag tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta 2881 gttaccgtga ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag 2941 gcactggctg gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc 3001 atgacgctgg cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc 3061 attcagccag ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct 3121 ggatacatgg ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa 3181 gcaatgaact ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag 3241 actggagaga ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct 3301 gtgtggcagg aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag 3361 ttccgcttac agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag 3421 gagtctggta tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact 3481 gtagtgtggg cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc 3541 ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac 3601 acatatgagt cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac 3661 gagtctcaat tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac 3721 atcttagagt cggacttcgc gttcgcataa tactagagcc aggcatcaaa taaaacgaaa 3781 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 3841 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //