LOCUS BBa_K228231 3888 bp DNA linear 22-Nov-2024 DEFINITION Sal->rbs->T7ptag->Terminator ACCESSION BBa_K228231 VERSION BBa_K228231.1 FEATURES Location/Qualifiers CDS 1..903 /label=NahR promoter 904..993 /label=Pr promoter promoter 982..1050 /label=pSal promoter 1..1052 /label=BBa_K228004 misc_feature 1065..1068 /label=conserved RBS 1061..1072 /label=BBa_B0034 CDS 1079..3751 /label=cds CDS 1079..3751 /label=BBa_K228000 stem_loop 3771..3814 /label=stem_loop misc_feature 3760..3839 /label=BBa_B0010 terminator 3760..3839 /label=BBa_B0010 stem_loop 3855..3874 /label=T7 TE misc_feature 3881..3881 /label=stop polyA_site 3875..3888 /label=polya misc_feature 3848..3888 /label=BBa_B0012 terminator 3848..3888 /label=BBa_B0012 ORIGIN 1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg 61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa 121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag 181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat 241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc 301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt 361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa 421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa 481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg 541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc 601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt 661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg 721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt 781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg 841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc 901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga 961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca 1021 agtcatcaat aaagccatca cgagtaccat agtactagag aaagaggaga aatactagat 1081 gaccatgatt accgtgcact agaataccat taacattgct aagaacgact tctctgacat 1141 cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg 1201 cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat 1261 gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat 1321 cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc 1381 taagcgcggc aagcgcccga cagccttcca gttcctgtag gaaatcaagc cggaagccgt 1441 agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt 1501 tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat 1561 ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt 1621 agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg 1681 tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt 1741 acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc 1801 tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat 1861 cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc 1921 tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct 1981 ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc 2041 tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt 2101 cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc 2161 gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc ctgaggctct 2221 caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg 2281 ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat 2341 ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc 2401 gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa 2461 ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc 2521 gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa 2581 gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt 2641 ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct 2701 ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt 2761 aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt 2821 tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt 2881 agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa 2941 ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt 3001 catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac 3061 cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc 3121 tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga 3181 agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa 3241 gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc 3301 tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca 3361 gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca 3421 ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac 3481 tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt 3541 cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga 3601 cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca 3661 cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga 3721 catcttagag tcggacttcg cgttcgcata atactagagc caggcatcaa ataaaacgaa 3781 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 3841 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //