LOCUS       BBa_K228247              986 bp    DNA     linear       07-May-2025
DEFINITION  T7 promoter->RBS->cI->Terminator
ACCESSION   BBa_K228247
VERSION     BBa_K228247.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    61..64
                     /label=conserved
     RBS             55..68
                     /label=BBa_B0031
     CDS             78..785
                     /label=cI lambda
     misc_feature    786..818
                     /label=LVA
     misc_feature    825..849
                     /label=Help:Barcodes
     CDS             75..849
                     /label=BBa_C0051
     stem_loop       869..912
                     /label=stem_loop
     misc_feature    858..937
                     /label=BBa_B0010
     terminator      858..937
                     /label=BBa_B0010
     stem_loop       953..972
                     /label=T7 TE
     misc_feature    979..979
                     /label=stop
     polyA_site      973..986
                     /label=polya
     misc_feature    946..986
                     /label=BBa_B0012
     terminator      946..986
                     /label=BBa_B0012
     terminator      858..986
                     /label=BBa_B0015
     misc_feature    75..986
                     /label=BBa_K228236
     misc_feature    55..986
                     /label=BBa_K228238
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtcacac
       61 aggaaaccta ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg
      121 cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat
      181 ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca
      241 atgcattaaa tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag
      301 aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc
      361 cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct
      421 cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa
      481 aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag
      541 gctccaagcc aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg
      601 agccaggtga tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga
      661 tcagggatag cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat
      721 gcaatgagag ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt
      781 ttggcgctgc aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt
      841 gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      901 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      961 ttcgggtggg cctttctgcg tttata
//