LOCUS       BBa_K228249              983 bp    DNA     linear       29-Dec-2025
DEFINITION  T7 promoter->RBS->cI->Terminator
ACCESSION   BBa_K228249
VERSION     BBa_K228249.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     RBS             55..65
                     /label=RBS-4\Weaker
     RBS             61..64
                     /label=RBS
     misc_feature    55..65
                     /label=BBa_B0033
     RBS             55..65
                     /label=BBa_B0033
     CDS             75..782
                     /label=cI lambda
     misc_feature    783..815
                     /label=LVA
     misc_feature    822..846
                     /label=Help:Barcodes
     CDS             72..846
                     /label=BBa_C0051
     stem_loop       866..909
                     /label=stem_loop
     misc_feature    855..934
                     /label=BBa_B0010
     terminator      855..934
                     /label=BBa_B0010
     stem_loop       950..969
                     /label=T7 TE
     misc_feature    976..976
                     /label=stop
     polyA_site      970..983
                     /label=polya
     misc_feature    943..983
                     /label=BBa_B0012
     terminator      943..983
                     /label=BBa_B0012
     terminator      855..983
                     /label=BBa_B0015
     misc_feature    72..983
                     /label=BBa_K228236
     misc_feature    55..983
                     /label=BBa_K228240
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtcacac
       61 aggactacta gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac
      121 gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg
      181 tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg
      241 cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat
      301 ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt
      361 cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac
      421 ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag
      481 ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct
      541 ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc
      601 caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca
      661 gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca
      721 atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg
      781 gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta
      841 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      901 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      961 gggtgggcct ttctgcgttt ata
//