LOCUS       BBa_K228251              987 bp    DNA     linear       22-Dec-2024
DEFINITION  T7 promoter->RBS->cI->Terminator
ACCESSION   BBa_K228251
VERSION     BBa_K228251.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     RBS             55..69
                     /label=Reverse RBS
     RBS             55..69
                     /label=BBa_J44001
     CDS             79..786
                     /label=cI lambda
     misc_feature    787..819
                     /label=LVA
     misc_feature    826..850
                     /label=Help:Barcodes
     CDS             76..850
                     /label=BBa_C0051
     stem_loop       870..913
                     /label=stem_loop
     misc_feature    859..938
                     /label=BBa_B0010
     terminator      859..938
                     /label=BBa_B0010
     stem_loop       954..973
                     /label=T7 TE
     misc_feature    980..980
                     /label=stop
     polyA_site      974..987
                     /label=polya
     misc_feature    947..987
                     /label=BBa_B0012
     terminator      947..987
                     /label=BBa_B0012
     terminator      859..987
                     /label=BBa_B0015
     misc_feature    76..987
                     /label=BBa_K228236
     misc_feature    55..987
                     /label=BBa_K228242
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtttctc
       61 ctctttaatt actagatgag cacaaaaaag aaaccattaa cacaagagca gcttgaggac
      121 gcacgtcgcc ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa
      181 tctgtcgcag acaagatggg gatggggcag tcaggcgttg gtgctttatt taatggcatc
      241 aatgcattaa atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa
      301 gaatttagcc cttcaatcgc cagagaaatc tacgagatgt atgaagcggt tagtatgcag
      361 ccgtcactta gaagtgagta tgagtaccct gttttttctc atgttcaggc agggatgttc
      421 tcacctgagc ttagaacctt taccaaaggt gatgcggaga gatgggtaag cacaaccaaa
      481 aaagccagtg attctgcatt ctggcttgag gttgaaggta attccatgac cgcaccaaca
      541 ggctccaagc caagctttcc tgacggaatg ttaattctcg ttgaccctga gcaggctgtt
      601 gagccaggtg atttctgcat agccagactt gggggtgatg agtttacctt caagaaactg
      661 atcagggata gcggtcaggt gtttttacaa ccactaaacc cacagtaccc aatgatccca
      721 tgcaatgaga gttgttccgt tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg
      781 tttggcgctg caaacgacga aaactacgct ttagtagctt aataacgctg atagtgctag
      841 tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      901 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      961 cttcgggtgg gcctttctgc gtttata
//