LOCUS       BBa_K228253              984 bp    DNA     linear       22-Nov-2024
DEFINITION  T7 promoter->RBS->cI->Terminator
ACCESSION   BBa_K228253
VERSION     BBa_K228253.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     RBS             55..66
                     /label=BBa_J61107
     CDS             76..783
                     /label=cI lambda
     misc_feature    784..816
                     /label=LVA
     misc_feature    823..847
                     /label=Help:Barcodes
     CDS             73..847
                     /label=BBa_C0051
     stem_loop       867..910
                     /label=stem_loop
     misc_feature    856..935
                     /label=BBa_B0010
     terminator      856..935
                     /label=BBa_B0010
     stem_loop       951..970
                     /label=T7 TE
     misc_feature    977..977
                     /label=stop
     polyA_site      971..984
                     /label=polya
     misc_feature    944..984
                     /label=BBa_B0012
     terminator      944..984
                     /label=BBa_B0012
     terminator      856..984
                     /label=BBa_B0015
     misc_feature    73..984
                     /label=BBa_K228236
     misc_feature    55..984
                     /label=BBa_K228244
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaagaa
       61 gagacttact agatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca
      121 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct
      181 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat
      241 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa
      301 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg
      361 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca
      421 cctgagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa
      481 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc
      541 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag
      601 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc
      661 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc
      721 aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt
      781 ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt
      841 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      901 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      961 cgggtgggcc tttctgcgtt tata
//