LOCUS BBa_K228255 5459 bp DNA linear 22-May-2024 DEFINITION AND GATE AraC+SupD+Sal+RBS(B0030)+T7ptag ACCESSION BBa_K228255 VERSION BBa_K228255.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 misc_feature 1288..1423 /label=BBa_K228001 stem_loop 1443..1486 /label=stem_loop misc_feature 1432..1511 /label=BBa_B0010 terminator 1432..1511 /label=BBa_B0010 stem_loop 1527..1546 /label=T7 TE misc_feature 1553..1553 /label=stop polyA_site 1547..1560 /label=polya misc_feature 1520..1560 /label=BBa_B0012 terminator 1520..1560 /label=BBa_B0012 misc_feature 1288..1560 /label=BBa_K228100 CDS 1569..2471 /label=NahR promoter 2472..2561 /label=Pr promoter promoter 2550..2618 /label=pSal promoter 1569..2620 /label=BBa_K228004 RBS 2629..2643 /label=RBS-1\Strong RBS 2636..2640 /label=RBS misc_feature 2629..2643 /label=BBa_B0030 RBS 2629..2643 /label=BBa_B0030 CDS 2650..5322 /label=cds CDS 2650..5322 /label=BBa_K228000 stem_loop 5342..5385 /label=stem_loop misc_feature 5331..5410 /label=BBa_B0010 terminator 5331..5410 /label=BBa_B0010 stem_loop 5426..5445 /label=T7 TE misc_feature 5452..5452 /label=stop polyA_site 5446..5459 /label=polya misc_feature 5419..5459 /label=BBa_B0012 terminator 5419..5459 /label=BBa_B0012 misc_feature 1569..5459 /label=BBa_K228227 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagcaa ttcggagaga tgccggagcg gctgaacgga 1321 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg 1381 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gccaggcatc 1441 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1501 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1561 tactagagtc aatccgtaaa caggtcaaac atcagttgcc gcaaccaaat attggctagg 1621 tccttgtggt acttcgcatg ccagaacatg ttgatggcta tttcaggcaa gacgactggg 1681 tgcggcaagg cgcttaggcc gaagggctcc acgcagcagt cggctaaacg tatcggcaca 1741 gtggcgagca gatcggtgcg ctggaggatg tggccaacgg cggcgaagtg cggcacttcc 1801 agacggatgt cgcgccggat gccgacccgt gtcatgtacg tgtccacctc gccgtggccg 1861 gtgccagcgg cgatgacacg cacgtggccg taggaacaga agcgctccag agtcaggggt 1921 tcgcgggtga ctggatggtc cttgcgacat aggcacacgt agtgattctg gagcagccgg 1981 cgctgaaaga agccagtttg cagattggga agcaggccca cggccaagtc cacggttccg 2041 ttctgcaagg cctgcatcag gctcatcgaa ctgtcgcgca ccgtactgat cacgcaattg 2101 ggggcctggt gagccagcac atccatcagc cgcggcatga agtagatctc gccaatgtcg 2161 gtcatggcca gggtgaaggt acgctcgctg gtcagcggat cgaagctttc atggtgctgt 2221 agggcgttgc gcagtgcgtg catggccgaa gtgacgggct cggccagatg cgcggcatag 2281 ggtgtgggtt ccattccctg atgtgtgcgc acgaagagtg ggtcctgtag cgaggtgcgc 2341 aggcgtttca gcgcattgct cacggcaggc tgggtcaggc ccaggttctc cgcagtgata 2401 gagacgcgtc tgtcgaccag caactggttg aacaccacca gcaggtttaa atccaggtca 2461 cgcagttcca tggggcctcg cttgggttat tgctggtgcc cggccgggcg caatattcat 2521 gttgatgatt tattatatat cgagtggtgt atttatcaat attgtttgct ccgttatcgt 2581 tattaacaag tcatcaataa agccatcacg agtaccatag tactagagat taaagaggag 2641 aaatactaga tgaccatgat taccgtgcac tagaatacca ttaacattgc taagaacgac 2701 ttctctgaca tcgaactggc tgctatcccg ttcaacactc tggctgacca ttacggtgag 2761 cgtttagctc gcgaacagtt ggcccttgag catgagtctt acgagatggg tgaagcacgc 2821 ttccgcaaga tgtttgagcg tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc 2881 aagcctctca tcactaccct actccctaag atgattgcac gcatcaacga ctggtttgag 2941 gaagtgaaag ctaagcgcgg caagcgcccg acagccttcc agttcctgta ggaaatcaag 3001 ccggaagccg tagcgtacat caccattaag accactctgg cttgcctaac cagtgctgac 3061 aatacaaccg ttcaggctgt agcaagcgca atcggtcggg ccattgagga cgaggctcgc 3121 ttcggtcgta tccgtgacct tgaagctaag cacttcaaga aaaacgttga ggaacaactc 3181 aacaagcgcg tagggcacgt ctacaagaaa gcatttatgc aagttgtcga ggctgacatg 3241 ctctctaagg gtctactcgg tggcgaggcg tggtcttcgt ggcataagga agactctatt 3301 catgtaggag tacgctgcat cgagatgctc attgagtcaa ccggaatggt tagcttacac 3361 cgccaaaatg ctggcgtagt aggtcaagac tctgagacta tcgaactcgc acctgaatac 3421 gctgaggcta tcgcaacccg tgcaggtgcg ctggctggca tctctccgat gttccaacct 3481 tgcgtagttc ctcctaagcc gtggactggc attactggtg gtggctattg ggctaacggt 3541 cgtcgtcctc tggcgctggt gcgtactcac agtaagaaag cactgatgcg ctacgaagac 3601 gtttacatgc ctgaggtgta caaagcgatt aacattgcgc aaaacaccgc atggaaaatc 3661 aacaagaaag tcctagcggt cgccaacgta atcaccaagt ggaagcattg tccggtcgag 3721 gacatccctg cgattgagcg tgaagaactc ccgatgaaac cggaagacat cgacatgaat 3781 cctgaggctc tcaccgcgtg gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct 3841 cgcaagtctc gccgtatcag ccttgagttc atgcttgagc aagccaataa gtttgctaac 3901 cataaggcca tctggttccc ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca 3961 atgttcaacc cgcaaggtaa cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa 4021 ccaatcggta aggaaggtta ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc 4081 gataaggttc cgttccctga gcgcatcaag ttcattgagg aaaaccacga gaacatcatg 4141 gcttgcgcta agtctccact ggagaacact tggtgggctg agcaagattc tccgttctgc 4201 ttccttgcgt tctgctttga gtacgctggg gtacagcacc acggcctgag ctataactgc 4261 tcccttccgc tggcgtttga cgggtcttgc tctggcatcc agcacttctc cgcgatgctc 4321 cgagatgagg taggtggtcg cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc 4381 tacgggattg ttgctaagaa agtcaacgag attctacaag cagacgcaat caatgggacc 4441 gataacgaag tagttaccgt gaccgatgag aacactggtg aaatctctga gaaagtcaag 4501 ctgggcacta aggcactggc tggtcaatgg ctggcttacg gtgttactcg cagtgtgact 4561 aagcgttcag tcatgacgct ggcttacggg tccaaagagt tcggcttccg tcaacaagtg 4621 ctggaagata ccattcagcc agctattgat tccggcaagg gtctgatgtt cactcagccg 4681 aatcaggctg ctggatacat ggctaagctg atttgggaat ctgtgagcgt gacggtggta 4741 gctgcggttg aagcaatgaa ctggcttaag tctgctgcta agctgctggc tgctgaggtc 4801 aaagataaga agactggaga gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct 4861 gatggtttcc ctgtgtggca ggaatacaag aagcctattc agacgcgctt gaacctgatg 4921 ttcctcggtc agttccgctt acagcctacc attaacacca acaaagatag cgagattgat 4981 gcacacaaac aggagtctgg tatcgctcct aactttgtac acagccaaga cggtagccac 5041 cttcgtaaga ctgtagtgtg ggcacacgag aagtacggaa tcgaatcttt tgcactgatt 5101 cacgactcct tcggtaccat tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa 5161 actatggttg acacatatga gtcttgtgat gtactggctg atttctacga ccagttcgct 5221 gaccagttgc acgagtctca attggacaaa atgccagcac ttccggctaa aggtaacttg 5281 aacctccgtg acatcttaga gtcggacttc gcgttcgcat aatactagag ccaggcatca 5341 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 5401 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //