LOCUS BBa_K228256 5458 bp DNA linear 22-Dec-2024 DEFINITION AND GATE AraC+SupD+Sal+RBS(B0031)+T7ptag ACCESSION BBa_K228256 VERSION BBa_K228256.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 misc_feature 1288..1423 /label=BBa_K228001 stem_loop 1443..1486 /label=stem_loop misc_feature 1432..1511 /label=BBa_B0010 terminator 1432..1511 /label=BBa_B0010 stem_loop 1527..1546 /label=T7 TE misc_feature 1553..1553 /label=stop polyA_site 1547..1560 /label=polya misc_feature 1520..1560 /label=BBa_B0012 terminator 1520..1560 /label=BBa_B0012 misc_feature 1288..1560 /label=BBa_K228100 CDS 1569..2471 /label=NahR promoter 2472..2561 /label=Pr promoter promoter 2550..2618 /label=pSal promoter 1569..2620 /label=BBa_K228004 misc_feature 2635..2638 /label=conserved RBS 2629..2642 /label=BBa_B0031 CDS 2649..5321 /label=cds CDS 2649..5321 /label=BBa_K228000 stem_loop 5341..5384 /label=stem_loop misc_feature 5330..5409 /label=BBa_B0010 terminator 5330..5409 /label=BBa_B0010 stem_loop 5425..5444 /label=T7 TE misc_feature 5451..5451 /label=stop polyA_site 5445..5458 /label=polya misc_feature 5418..5458 /label=BBa_B0012 terminator 5418..5458 /label=BBa_B0012 misc_feature 1569..5458 /label=BBa_K228228 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagcaa ttcggagaga tgccggagcg gctgaacgga 1321 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg 1381 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gccaggcatc 1441 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1501 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1561 tactagagtc aatccgtaaa caggtcaaac atcagttgcc gcaaccaaat attggctagg 1621 tccttgtggt acttcgcatg ccagaacatg ttgatggcta tttcaggcaa gacgactggg 1681 tgcggcaagg cgcttaggcc gaagggctcc acgcagcagt cggctaaacg tatcggcaca 1741 gtggcgagca gatcggtgcg ctggaggatg tggccaacgg cggcgaagtg cggcacttcc 1801 agacggatgt cgcgccggat gccgacccgt gtcatgtacg tgtccacctc gccgtggccg 1861 gtgccagcgg cgatgacacg cacgtggccg taggaacaga agcgctccag agtcaggggt 1921 tcgcgggtga ctggatggtc cttgcgacat aggcacacgt agtgattctg gagcagccgg 1981 cgctgaaaga agccagtttg cagattggga agcaggccca cggccaagtc cacggttccg 2041 ttctgcaagg cctgcatcag gctcatcgaa ctgtcgcgca ccgtactgat cacgcaattg 2101 ggggcctggt gagccagcac atccatcagc cgcggcatga agtagatctc gccaatgtcg 2161 gtcatggcca gggtgaaggt acgctcgctg gtcagcggat cgaagctttc atggtgctgt 2221 agggcgttgc gcagtgcgtg catggccgaa gtgacgggct cggccagatg cgcggcatag 2281 ggtgtgggtt ccattccctg atgtgtgcgc acgaagagtg ggtcctgtag cgaggtgcgc 2341 aggcgtttca gcgcattgct cacggcaggc tgggtcaggc ccaggttctc cgcagtgata 2401 gagacgcgtc tgtcgaccag caactggttg aacaccacca gcaggtttaa atccaggtca 2461 cgcagttcca tggggcctcg cttgggttat tgctggtgcc cggccgggcg caatattcat 2521 gttgatgatt tattatatat cgagtggtgt atttatcaat attgtttgct ccgttatcgt 2581 tattaacaag tcatcaataa agccatcacg agtaccatag tactagagtc acacaggaaa 2641 cctactagat gaccatgatt accgtgcact agaataccat taacattgct aagaacgact 2701 tctctgacat cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc 2761 gtttagctcg cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct 2821 tccgcaagat gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca 2881 agcctctcat cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg 2941 aagtgaaagc taagcgcggc aagcgcccga cagccttcca gttcctgtag gaaatcaagc 3001 cggaagccgt agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca 3061 atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct 3121 tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca 3181 acaagcgcgt agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc 3241 tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc 3301 atgtaggagt acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc 3361 gccaaaatgc tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg 3421 ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt 3481 gcgtagttcc tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc 3541 gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg 3601 tttacatgcc tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca 3661 acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg 3721 acatccctgc gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc 3781 ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc 3841 gcaagtctcg ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc 3901 ataaggccat ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa 3961 tgttcaaccc gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac 4021 caatcggtaa ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg 4081 ataaggttcc gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg 4141 cttgcgctaa gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct 4201 tccttgcgtt ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct 4261 cccttccgct ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc 4321 gagatgaggt aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct 4381 acgggattgt tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg 4441 ataacgaagt agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc 4501 tgggcactaa ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta 4561 agcgttcagt catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc 4621 tggaagatac cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga 4681 atcaggctgc tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag 4741 ctgcggttga agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca 4801 aagataagaa gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg 4861 atggtttccc tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt 4921 tcctcggtca gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg 4981 cacacaaaca ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc 5041 ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc 5101 acgactcctt cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa 5161 ctatggttga cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg 5221 accagttgca cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga 5281 acctccgtga catcttagag tcggacttcg cgttcgcata atactagagc caggcatcaa 5341 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 5401 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //