LOCUS BBa_K228257 5457 bp DNA linear 23-May-2024 DEFINITION AND GATE AraC+SupD+Sal+RBS(B0032)+T7ptag ACCESSION BBa_K228257 VERSION BBa_K228257.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 misc_feature 1288..1423 /label=BBa_K228001 stem_loop 1443..1486 /label=stem_loop misc_feature 1432..1511 /label=BBa_B0010 terminator 1432..1511 /label=BBa_B0010 stem_loop 1527..1546 /label=T7 TE misc_feature 1553..1553 /label=stop polyA_site 1547..1560 /label=polya misc_feature 1520..1560 /label=BBa_B0012 terminator 1520..1560 /label=BBa_B0012 misc_feature 1288..1560 /label=BBa_K228100 CDS 1569..2471 /label=NahR promoter 2472..2561 /label=Pr promoter promoter 2550..2618 /label=pSal promoter 1569..2620 /label=BBa_K228004 RBS 2629..2641 /label=RBS-3\Weak RBS 2635..2638 /label=RBS misc_feature 2629..2641 /label=BBa_B0032 RBS 2629..2641 /label=BBa_B0032 CDS 2648..5320 /label=cds CDS 2648..5320 /label=BBa_K228000 stem_loop 5340..5383 /label=stem_loop misc_feature 5329..5408 /label=BBa_B0010 terminator 5329..5408 /label=BBa_B0010 stem_loop 5424..5443 /label=T7 TE misc_feature 5450..5450 /label=stop polyA_site 5444..5457 /label=polya misc_feature 5417..5457 /label=BBa_B0012 terminator 5417..5457 /label=BBa_B0012 misc_feature 1569..5457 /label=BBa_K228229 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagcaa ttcggagaga tgccggagcg gctgaacgga 1321 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg 1381 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gccaggcatc 1441 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1501 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1561 tactagagtc aatccgtaaa caggtcaaac atcagttgcc gcaaccaaat attggctagg 1621 tccttgtggt acttcgcatg ccagaacatg ttgatggcta tttcaggcaa gacgactggg 1681 tgcggcaagg cgcttaggcc gaagggctcc acgcagcagt cggctaaacg tatcggcaca 1741 gtggcgagca gatcggtgcg ctggaggatg tggccaacgg cggcgaagtg cggcacttcc 1801 agacggatgt cgcgccggat gccgacccgt gtcatgtacg tgtccacctc gccgtggccg 1861 gtgccagcgg cgatgacacg cacgtggccg taggaacaga agcgctccag agtcaggggt 1921 tcgcgggtga ctggatggtc cttgcgacat aggcacacgt agtgattctg gagcagccgg 1981 cgctgaaaga agccagtttg cagattggga agcaggccca cggccaagtc cacggttccg 2041 ttctgcaagg cctgcatcag gctcatcgaa ctgtcgcgca ccgtactgat cacgcaattg 2101 ggggcctggt gagccagcac atccatcagc cgcggcatga agtagatctc gccaatgtcg 2161 gtcatggcca gggtgaaggt acgctcgctg gtcagcggat cgaagctttc atggtgctgt 2221 agggcgttgc gcagtgcgtg catggccgaa gtgacgggct cggccagatg cgcggcatag 2281 ggtgtgggtt ccattccctg atgtgtgcgc acgaagagtg ggtcctgtag cgaggtgcgc 2341 aggcgtttca gcgcattgct cacggcaggc tgggtcaggc ccaggttctc cgcagtgata 2401 gagacgcgtc tgtcgaccag caactggttg aacaccacca gcaggtttaa atccaggtca 2461 cgcagttcca tggggcctcg cttgggttat tgctggtgcc cggccgggcg caatattcat 2521 gttgatgatt tattatatat cgagtggtgt atttatcaat attgtttgct ccgttatcgt 2581 tattaacaag tcatcaataa agccatcacg agtaccatag tactagagtc acacaggaaa 2641 gtactagatg accatgatta ccgtgcacta gaataccatt aacattgcta agaacgactt 2701 ctctgacatc gaactggctg ctatcccgtt caacactctg gctgaccatt acggtgagcg 2761 tttagctcgc gaacagttgg cccttgagca tgagtcttac gagatgggtg aagcacgctt 2821 ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt gcggataacg ctgccgccaa 2881 gcctctcatc actaccctac tccctaagat gattgcacgc atcaacgact ggtttgagga 2941 agtgaaagct aagcgcggca agcgcccgac agccttccag ttcctgtagg aaatcaagcc 3001 ggaagccgta gcgtacatca ccattaagac cactctggct tgcctaacca gtgctgacaa 3061 tacaaccgtt caggctgtag caagcgcaat cggtcgggcc attgaggacg aggctcgctt 3121 cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa aacgttgagg aacaactcaa 3181 caagcgcgta gggcacgtct acaagaaagc atttatgcaa gttgtcgagg ctgacatgct 3241 ctctaagggt ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag actctattca 3301 tgtaggagta cgctgcatcg agatgctcat tgagtcaacc ggaatggtta gcttacaccg 3361 ccaaaatgct ggcgtagtag gtcaagactc tgagactatc gaactcgcac ctgaatacgc 3421 tgaggctatc gcaacccgtg caggtgcgct ggctggcatc tctccgatgt tccaaccttg 3481 cgtagttcct cctaagccgt ggactggcat tactggtggt ggctattggg ctaacggtcg 3541 tcgtcctctg gcgctggtgc gtactcacag taagaaagca ctgatgcgct acgaagacgt 3601 ttacatgcct gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa 3661 caagaaagtc ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc cggtcgagga 3721 catccctgcg attgagcgtg aagaactccc gatgaaaccg gaagacatcg acatgaatcc 3781 tgaggctctc accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg 3841 caagtctcgc cgtatcagcc ttgagttcat gcttgagcaa gccaataagt ttgctaacca 3901 taaggccatc tggttccctt acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat 3961 gttcaacccg caaggtaacg atatgaccaa aggactgctt acgctggcga aaggtaaacc 4021 aatcggtaag gaaggttact actggctgaa aatccacggt gcaaactgtg cgggtgtcga 4081 taaggttccg ttccctgagc gcatcaagtt cattgaggaa aaccacgaga acatcatggc 4141 ttgcgctaag tctccactgg agaacacttg gtgggctgag caagattctc cgttctgctt 4201 ccttgcgttc tgctttgagt acgctggggt acagcaccac ggcctgagct ataactgctc 4261 ccttccgctg gcgtttgacg ggtcttgctc tggcatccag cacttctccg cgatgctccg 4321 agatgaggta ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc aggacatcta 4381 cgggattgtt gctaagaaag tcaacgagat tctacaagca gacgcaatca atgggaccga 4441 taacgaagta gttaccgtga ccgatgagaa cactggtgaa atctctgaga aagtcaagct 4501 gggcactaag gcactggctg gtcaatggct ggcttacggt gttactcgca gtgtgactaa 4561 gcgttcagtc atgacgctgg cttacgggtc caaagagttc ggcttccgtc aacaagtgct 4621 ggaagatacc attcagccag ctattgattc cggcaagggt ctgatgttca ctcagccgaa 4681 tcaggctgct ggatacatgg ctaagctgat ttgggaatct gtgagcgtga cggtggtagc 4741 tgcggttgaa gcaatgaact ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa 4801 agataagaag actggagaga ttcttcgcaa gcgttgcgct gtgcattggg taactcctga 4861 tggtttccct gtgtggcagg aatacaagaa gcctattcag acgcgcttga acctgatgtt 4921 cctcggtcag ttccgcttac agcctaccat taacaccaac aaagatagcg agattgatgc 4981 acacaaacag gagtctggta tcgctcctaa ctttgtacac agccaagacg gtagccacct 5041 tcgtaagact gtagtgtggg cacacgagaa gtacggaatc gaatcttttg cactgattca 5101 cgactccttc ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac 5161 tatggttgac acatatgagt cttgtgatgt actggctgat ttctacgacc agttcgctga 5221 ccagttgcac gagtctcaat tggacaaaat gccagcactt ccggctaaag gtaacttgaa 5281 cctccgtgac atcttagagt cggacttcgc gttcgcataa tactagagcc aggcatcaaa 5341 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 5401 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //