LOCUS BBa_K228258 5455 bp DNA linear 25-Nov-2024 DEFINITION AND GATE AraC+SupD+Sal+RBS(B0033)+T7ptag ACCESSION BBa_K228258 VERSION BBa_K228258.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 misc_feature 1288..1423 /label=BBa_K228001 stem_loop 1443..1486 /label=stem_loop misc_feature 1432..1511 /label=BBa_B0010 terminator 1432..1511 /label=BBa_B0010 stem_loop 1527..1546 /label=T7 TE misc_feature 1553..1553 /label=stop polyA_site 1547..1560 /label=polya misc_feature 1520..1560 /label=BBa_B0012 terminator 1520..1560 /label=BBa_B0012 misc_feature 1288..1560 /label=BBa_K228100 CDS 1569..2471 /label=NahR promoter 2472..2561 /label=Pr promoter promoter 2550..2618 /label=pSal promoter 1569..2620 /label=BBa_K228004 RBS 2629..2639 /label=RBS-4\Weaker RBS 2635..2638 /label=RBS misc_feature 2629..2639 /label=BBa_B0033 RBS 2629..2639 /label=BBa_B0033 CDS 2646..5318 /label=cds CDS 2646..5318 /label=BBa_K228000 stem_loop 5338..5381 /label=stem_loop misc_feature 5327..5406 /label=BBa_B0010 terminator 5327..5406 /label=BBa_B0010 stem_loop 5422..5441 /label=T7 TE misc_feature 5448..5448 /label=stop polyA_site 5442..5455 /label=polya misc_feature 5415..5455 /label=BBa_B0012 terminator 5415..5455 /label=BBa_B0012 misc_feature 1569..5455 /label=BBa_K228230 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagcaa ttcggagaga tgccggagcg gctgaacgga 1321 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg 1381 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gccaggcatc 1441 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1501 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1561 tactagagtc aatccgtaaa caggtcaaac atcagttgcc gcaaccaaat attggctagg 1621 tccttgtggt acttcgcatg ccagaacatg ttgatggcta tttcaggcaa gacgactggg 1681 tgcggcaagg cgcttaggcc gaagggctcc acgcagcagt cggctaaacg tatcggcaca 1741 gtggcgagca gatcggtgcg ctggaggatg tggccaacgg cggcgaagtg cggcacttcc 1801 agacggatgt cgcgccggat gccgacccgt gtcatgtacg tgtccacctc gccgtggccg 1861 gtgccagcgg cgatgacacg cacgtggccg taggaacaga agcgctccag agtcaggggt 1921 tcgcgggtga ctggatggtc cttgcgacat aggcacacgt agtgattctg gagcagccgg 1981 cgctgaaaga agccagtttg cagattggga agcaggccca cggccaagtc cacggttccg 2041 ttctgcaagg cctgcatcag gctcatcgaa ctgtcgcgca ccgtactgat cacgcaattg 2101 ggggcctggt gagccagcac atccatcagc cgcggcatga agtagatctc gccaatgtcg 2161 gtcatggcca gggtgaaggt acgctcgctg gtcagcggat cgaagctttc atggtgctgt 2221 agggcgttgc gcagtgcgtg catggccgaa gtgacgggct cggccagatg cgcggcatag 2281 ggtgtgggtt ccattccctg atgtgtgcgc acgaagagtg ggtcctgtag cgaggtgcgc 2341 aggcgtttca gcgcattgct cacggcaggc tgggtcaggc ccaggttctc cgcagtgata 2401 gagacgcgtc tgtcgaccag caactggttg aacaccacca gcaggtttaa atccaggtca 2461 cgcagttcca tggggcctcg cttgggttat tgctggtgcc cggccgggcg caatattcat 2521 gttgatgatt tattatatat cgagtggtgt atttatcaat attgtttgct ccgttatcgt 2581 tattaacaag tcatcaataa agccatcacg agtaccatag tactagagtc acacaggact 2641 actagatgac catgattacc gtgcactaga ataccattaa cattgctaag aacgacttct 2701 ctgacatcga actggctgct atcccgttca acactctggc tgaccattac ggtgagcgtt 2761 tagctcgcga acagttggcc cttgagcatg agtcttacga gatgggtgaa gcacgcttcc 2821 gcaagatgtt tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct gccgccaagc 2881 ctctcatcac taccctactc cctaagatga ttgcacgcat caacgactgg tttgaggaag 2941 tgaaagctaa gcgcggcaag cgcccgacag ccttccagtt cctgtaggaa atcaagccgg 3001 aagccgtagc gtacatcacc attaagacca ctctggcttg cctaaccagt gctgacaata 3061 caaccgttca ggctgtagca agcgcaatcg gtcgggccat tgaggacgag gctcgcttcg 3121 gtcgtatccg tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa caactcaaca 3181 agcgcgtagg gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct gacatgctct 3241 ctaagggtct actcggtggc gaggcgtggt cttcgtggca taaggaagac tctattcatg 3301 taggagtacg ctgcatcgag atgctcattg agtcaaccgg aatggttagc ttacaccgcc 3361 aaaatgctgg cgtagtaggt caagactctg agactatcga actcgcacct gaatacgctg 3421 aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc caaccttgcg 3481 tagttcctcc taagccgtgg actggcatta ctggtggtgg ctattgggct aacggtcgtc 3541 gtcctctggc gctggtgcgt actcacagta agaaagcact gatgcgctac gaagacgttt 3601 acatgcctga ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg aaaatcaaca 3661 agaaagtcct agcggtcgcc aacgtaatca ccaagtggaa gcattgtccg gtcgaggaca 3721 tccctgcgat tgagcgtgaa gaactcccga tgaaaccgga agacatcgac atgaatcctg 3781 aggctctcac cgcgtggaaa cgtgctgccg ctgctgtgta ccgcaaggac aaggctcgca 3841 agtctcgccg tatcagcctt gagttcatgc ttgagcaagc caataagttt gctaaccata 3901 aggccatctg gttcccttac aacatggact ggcgcggtcg tgtttacgct gtgtcaatgt 3961 tcaacccgca aggtaacgat atgaccaaag gactgcttac gctggcgaaa ggtaaaccaa 4021 tcggtaagga aggttactac tggctgaaaa tccacggtgc aaactgtgcg ggtgtcgata 4081 aggttccgtt ccctgagcgc atcaagttca ttgaggaaaa ccacgagaac atcatggctt 4141 gcgctaagtc tccactggag aacacttggt gggctgagca agattctccg ttctgcttcc 4201 ttgcgttctg ctttgagtac gctggggtac agcaccacgg cctgagctat aactgctccc 4261 ttccgctggc gtttgacggg tcttgctctg gcatccagca cttctccgcg atgctccgag 4321 atgaggtagg tggtcgcgcg gttaacttgc ttcctagtga aaccgttcag gacatctacg 4381 ggattgttgc taagaaagtc aacgagattc tacaagcaga cgcaatcaat gggaccgata 4441 acgaagtagt taccgtgacc gatgagaaca ctggtgaaat ctctgagaaa gtcaagctgg 4501 gcactaaggc actggctggt caatggctgg cttacggtgt tactcgcagt gtgactaagc 4561 gttcagtcat gacgctggct tacgggtcca aagagttcgg cttccgtcaa caagtgctgg 4621 aagataccat tcagccagct attgattccg gcaagggtct gatgttcact cagccgaatc 4681 aggctgctgg atacatggct aagctgattt gggaatctgt gagcgtgacg gtggtagctg 4741 cggttgaagc aatgaactgg cttaagtctg ctgctaagct gctggctgct gaggtcaaag 4801 ataagaagac tggagagatt cttcgcaagc gttgcgctgt gcattgggta actcctgatg 4861 gtttccctgt gtggcaggaa tacaagaagc ctattcagac gcgcttgaac ctgatgttcc 4921 tcggtcagtt ccgcttacag cctaccatta acaccaacaa agatagcgag attgatgcac 4981 acaaacagga gtctggtatc gctcctaact ttgtacacag ccaagacggt agccaccttc 5041 gtaagactgt agtgtgggca cacgagaagt acggaatcga atcttttgca ctgattcacg 5101 actccttcgg taccattccg gctgacgctg cgaacctgtt caaagcagtg cgcgaaacta 5161 tggttgacac atatgagtct tgtgatgtac tggctgattt ctacgaccag ttcgctgacc 5221 agttgcacga gtctcaattg gacaaaatgc cagcacttcc ggctaaaggt aacttgaacc 5281 tccgtgacat cttagagtcg gacttcgcgt tcgcataata ctagagccag gcatcaaata 5341 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 5401 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //