##gff-version 3
##sequence-region BBa_K228258 1 5455
BBa_K228258	.	promoter	1	1279	.	+	0	ID=BBa_K228009;Name=BBa_K228009
BBa_K228258	.	promoter	1150	1279	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228258	.	engineered_region	1	1141	.	+	0	ID=BBa_K228008;Name=BBa_K228008;Parent=BBa_K228009
BBa_K228258	.	polyA_site	1128	1141	.	+	0	ID=annotation2020013;Name=polya;Parent=BBa_K228008
BBa_K228258	.	conserved_region	51	54	.	+	0	ID=annotation2020004;Name=conserved;Parent=BBa_K228008
BBa_K228258	.	promoter	27	32	.	+	0	ID=annotation2020001;Name=-10;Parent=BBa_K228008
BBa_K228258	.	engineered_region	1	38	.	+	0	ID=annotation2020002;Name=BBa_I14033;Parent=BBa_K228008
BBa_K228258	.	engineered_region	47	1141	.	+	0	ID=annotation2020015;Name=BBa_K228006;Parent=BBa_K228008
BBa_K228258	.	tag	941	973	.	+	0	ID=annotation2020007;Name=LVA;Parent=BBa_K228008
BBa_K228258	.	engineered_region	1013	1092	.	+	0	ID=annotation2020009;Name=BBa_B0010;Parent=BBa_K228008
BBa_K228258	.	engineered_region	1101	1141	.	+	0	ID=annotation2020011;Name=BBa_B0012;Parent=BBa_K228008
BBa_K228258	.	stop_codon	1134	1134	.	+	0	ID=annotation2020014;Name=stop;Parent=BBa_K228008
BBa_K228258	.	engineered_region	65	979	.	+	0	ID=annotation2020005;Name=BBa_C0080;Parent=BBa_K228008
BBa_K228258	.	engineered_region	1150	1279	.	+	0	ID=annotation2020016;Name=BBa_I13453;Parent=BBa_K228008
BBa_K228258	.	stem_loop	1108	1127	.	+	0	ID=annotation2020012;Name=T7 TE;Parent=BBa_K228008
BBa_K228258	.	engineered_region	47	58	.	+	0	ID=annotation2020003;Name=BBa_B0034;Parent=BBa_K228008
BBa_K228258	.	operator	1	38	.	+	0	ID=annotation2019999;Name=P(Cat);Parent=BBa_K228008
BBa_K228258	.	promoter	4	9	.	+	0	ID=annotation2020000;Name=-35;Parent=BBa_K228008
BBa_K228258	.	stem_loop	1024	1067	.	+	0	ID=annotation2020010;Name=stem_loop;Parent=BBa_K228008
BBa_K228258	.	CDS	65	940	.	+	0	ID=annotation2020006;Name=araC;Parent=BBa_K228008
BBa_K228258	.	engineered_region	980	1004	.	+	0	ID=annotation2020008;Name=barcode;Parent=BBa_K228008
BBa_K228258	.	promoter	1150	1279	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228258	.	terminator	1013	1092	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228258	.	stem_loop	1024	1067	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228258	.	engineered_region	1013	1092	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228258	.	engineered_region	1288	1560	.	+	0	ID=BBa_K228100;Name=BBa_K228100
BBa_K228258	.	terminator	1432	1511	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228100
BBa_K228258	.	stem_loop	1443	1486	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228258	.	engineered_region	1432	1511	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228258	.	terminator	1520	1560	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228100
BBa_K228258	.	polyA_site	1547	1560	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228258	.	stem_loop	1527	1546	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228258	.	stop_codon	1553	1553	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228258	.	engineered_region	1520	1560	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228258	.	mature_transcript_region	1288	1423	.	+	0	ID=BBa_K228001;Name=BBa_K228001;Parent=BBa_K228100
BBa_K228258	.	terminator	1101	1141	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228258	.	polyA_site	1128	1141	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228258	.	stem_loop	1108	1127	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228258	.	stop_codon	1134	1134	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228258	.	engineered_region	1101	1141	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228258	.	engineered_region	1569	5455	.	+	0	ID=BBa_K228230;Name=BBa_K228230
BBa_K228258	.	promoter	1569	2620	.	+	0	ID=BBa_K228004;Name=BBa_K228004;Parent=BBa_K228230
BBa_K228258	.	promoter	2550	2618	.	+	0	ID=annotation2075287;Name=pSal;Parent=BBa_K228004
BBa_K228258	.	promoter	2472	2561	.	+	0	ID=annotation2075286;Name=Pr promoter;Parent=BBa_K228004
BBa_K228258	.	CDS	1569	2471	.	+	0	ID=annotation2075285;Name=NahR;Parent=BBa_K228004
BBa_K228258	.	terminator	5327	5406	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228230
BBa_K228258	.	stem_loop	5338	5381	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228258	.	engineered_region	5327	5406	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228258	.	CDS	2646	5318	.	+	0	ID=BBa_K228000;Name=BBa_K228000;Parent=BBa_K228230
BBa_K228258	.	CDS	2646	5318	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228258	.	ribosome_entry_site	2629	2639	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_K228230
BBa_K228258	.	engineered_region	2629	2639	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K228258	.	ribosome_entry_site	2635	2638	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K228258	.	ribosome_entry_site	2629	2639	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K228258	.	terminator	5415	5455	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228230
BBa_K228258	.	polyA_site	5442	5455	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228258	.	stem_loop	5422	5441	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228258	.	stop_codon	5448	5448	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228258	.	engineered_region	5415	5455	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K228258
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatc
agtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttc
acaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaa
ccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcct
cgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctg
tgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatcc
atcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagct
ccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatc
cgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaa
acccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatat
cacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacc
tttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgg
gcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgt
ttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatg
ccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccg
tttttttgggctagctactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtagg
ggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagc
ttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcaatccgtaaa
caggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggct
atttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggca
cagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccg
gatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaa
cagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagca
gccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctg
catcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgc
ggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcat
ggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttc
cattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggc
tgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggttta
aatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatga
tttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatc
acgagtaccatagtactagagtcacacaggactactagatgaccatgattaccgtgcactagaataccattaacattgc
taagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagct
cgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaactta
aagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaa
cgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaa
gccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaa
gcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaa
cgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctc
tctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcg
agatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactat
cgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaacct
tgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctgg
tgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgc
gcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtc
gaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccg
cgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgct
tgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgct
gtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaagg
aaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagtt
cattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattct
ccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgc
tggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaa
cttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgca
atcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggca
ctaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggctta
cgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctg
atgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctg
cggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagat
tcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcag
acgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatg
cacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtg
ggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaac
ctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcg
ctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatctt
agagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tata