LOCUS BBa_K228259 5456 bp DNA linear 22-May-2024 DEFINITION AND GATE AraC+SupD+Sal+RBS(B0034)+T7ptag ACCESSION BBa_K228259 VERSION BBa_K228259.1 FEATURES Location/Qualifiers misc_feature 51..54 /label=conserved RBS 47..58 /label=BBa_B0034 stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 misc_feature 1288..1423 /label=BBa_K228001 stem_loop 1443..1486 /label=stem_loop misc_feature 1432..1511 /label=BBa_B0010 terminator 1432..1511 /label=BBa_B0010 stem_loop 1527..1546 /label=T7 TE misc_feature 1553..1553 /label=stop polyA_site 1547..1560 /label=polya misc_feature 1520..1560 /label=BBa_B0012 terminator 1520..1560 /label=BBa_B0012 misc_feature 1288..1560 /label=BBa_K228100 CDS 1569..2471 /label=NahR promoter 2472..2561 /label=Pr promoter promoter 2550..2618 /label=pSal promoter 1569..2620 /label=BBa_K228004 misc_feature 2633..2636 /label=conserved RBS 2629..2640 /label=BBa_B0034 CDS 2647..5319 /label=cds CDS 2647..5319 /label=BBa_K228000 stem_loop 5339..5382 /label=stem_loop misc_feature 5328..5407 /label=BBa_B0010 terminator 5328..5407 /label=BBa_B0010 stem_loop 5423..5442 /label=T7 TE misc_feature 5449..5449 /label=stop polyA_site 5443..5456 /label=polya misc_feature 5416..5456 /label=BBa_B0012 terminator 5416..5456 /label=BBa_B0012 misc_feature 1569..5456 /label=BBa_K228231 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagcaa ttcggagaga tgccggagcg gctgaacgga 1321 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg 1381 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gccaggcatc 1441 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1501 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1561 tactagagtc aatccgtaaa caggtcaaac atcagttgcc gcaaccaaat attggctagg 1621 tccttgtggt acttcgcatg ccagaacatg ttgatggcta tttcaggcaa gacgactggg 1681 tgcggcaagg cgcttaggcc gaagggctcc acgcagcagt cggctaaacg tatcggcaca 1741 gtggcgagca gatcggtgcg ctggaggatg tggccaacgg cggcgaagtg cggcacttcc 1801 agacggatgt cgcgccggat gccgacccgt gtcatgtacg tgtccacctc gccgtggccg 1861 gtgccagcgg cgatgacacg cacgtggccg taggaacaga agcgctccag agtcaggggt 1921 tcgcgggtga ctggatggtc cttgcgacat aggcacacgt agtgattctg gagcagccgg 1981 cgctgaaaga agccagtttg cagattggga agcaggccca cggccaagtc cacggttccg 2041 ttctgcaagg cctgcatcag gctcatcgaa ctgtcgcgca ccgtactgat cacgcaattg 2101 ggggcctggt gagccagcac atccatcagc cgcggcatga agtagatctc gccaatgtcg 2161 gtcatggcca gggtgaaggt acgctcgctg gtcagcggat cgaagctttc atggtgctgt 2221 agggcgttgc gcagtgcgtg catggccgaa gtgacgggct cggccagatg cgcggcatag 2281 ggtgtgggtt ccattccctg atgtgtgcgc acgaagagtg ggtcctgtag cgaggtgcgc 2341 aggcgtttca gcgcattgct cacggcaggc tgggtcaggc ccaggttctc cgcagtgata 2401 gagacgcgtc tgtcgaccag caactggttg aacaccacca gcaggtttaa atccaggtca 2461 cgcagttcca tggggcctcg cttgggttat tgctggtgcc cggccgggcg caatattcat 2521 gttgatgatt tattatatat cgagtggtgt atttatcaat attgtttgct ccgttatcgt 2581 tattaacaag tcatcaataa agccatcacg agtaccatag tactagagaa agaggagaaa 2641 tactagatga ccatgattac cgtgcactag aataccatta acattgctaa gaacgacttc 2701 tctgacatcg aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt 2761 ttagctcgcg aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc 2821 cgcaagatgt ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag 2881 cctctcatca ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa 2941 gtgaaagcta agcgcggcaa gcgcccgaca gccttccagt tcctgtagga aatcaagccg 3001 gaagccgtag cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat 3061 acaaccgttc aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc 3121 ggtcgtatcc gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac 3181 aagcgcgtag ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc 3241 tctaagggtc tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat 3301 gtaggagtac gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc 3361 caaaatgctg gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct 3421 gaggctatcg caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc 3481 gtagttcctc ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt 3541 cgtcctctgg cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt 3601 tacatgcctg aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac 3661 aagaaagtcc tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac 3721 atccctgcga ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct 3781 gaggctctca ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga caaggctcgc 3841 aagtctcgcc gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat 3901 aaggccatct ggttccctta caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg 3961 ttcaacccgc aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca 4021 atcggtaagg aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgat 4081 aaggttccgt tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct 4141 tgcgctaagt ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc 4201 cttgcgttct gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc 4261 cttccgctgg cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga 4321 gatgaggtag gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca ggacatctac 4381 gggattgttg ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat 4441 aacgaagtag ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg 4501 ggcactaagg cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag 4561 cgttcagtca tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg 4621 gaagatacca ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgaat 4681 caggctgctg gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct 4741 gcggttgaag caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa 4801 gataagaaga ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat 4861 ggtttccctg tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc 4921 ctcggtcagt tccgcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca 4981 cacaaacagg agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt 5041 cgtaagactg tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac 5101 gactccttcg gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact 5161 atggttgaca catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac 5221 cagttgcacg agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac 5281 ctccgtgaca tcttagagtc ggacttcgcg ttcgcataat actagagcca ggcatcaaat 5341 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 5401 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //