LOCUS       BBa_K228818              948 bp    DNA     linear       22-Dec-2024
DEFINITION  Pcat+RBS(B0034)+tetR(C0040)+terminator(B0015)+tetP(R0040)
ACCESSION   BBa_K228818
VERSION     BBa_K228818.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     misc_feature    51..54
                     /label=conserved
     RBS             47..58
                     /label=BBa_B0034
     CDS             68..684
                     /label=tetR
     misc_feature    685..718
                     /label=SsrA
     misc_feature    725..749
                     /label=Help:Barcodes
     CDS             65..749
                     /label=BBa_C0040
     stem_loop       769..812
                     /label=stem_loop
     misc_feature    758..837
                     /label=BBa_B0010
     terminator      758..837
                     /label=BBa_B0010
     stem_loop       853..872
                     /label=T7 TE
     misc_feature    879..879
                     /label=stop
     polyA_site      873..886
                     /label=polya
     misc_feature    846..886
                     /label=BBa_B0012
     terminator      846..886
                     /label=BBa_B0012
     terminator      758..886
                     /label=BBa_B0015
     misc_feature    895..913
                     /label=TetR 1
     misc_feature    895..948
                     /label=BBa_R0040
     promoter        914..919
                     /label=-35
     misc_feature    920..938
                     /label=TetR 2
     promoter        937..942
                     /label=-10
     promoter        895..948
                     /label=BBa_R0040
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata
       61 ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga
      121 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc
      181 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat
      241 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt
      301 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt
      361 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt
      421 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca
      481 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga
      541 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca
      601 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa
      661 acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta
      721 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag
      781 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      841 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      901 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcac
//