##gff-version 3
##sequence-region BBa_K228819 1 2448
BBa_K228819	.	engineered_region	1571	2448	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K228819	.	CDS	1592	2311	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K228819	.	CDS	1592	2311	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K228819	.	terminator	2408	2448	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K228819	.	engineered_region	2408	2448	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228819	.	stem_loop	2415	2434	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228819	.	stop_codon	2441	2441	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228819	.	polyA_site	2435	2448	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228819	.	terminator	2320	2399	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K228819	.	engineered_region	2320	2399	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228819	.	stem_loop	2331	2374	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228819	.	ribosome_entry_site	1571	1585	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K228819	.	ribosome_entry_site	1578	1582	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K228819	.	engineered_region	1571	1585	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K228819	.	ribosome_entry_site	1571	1585	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K228819	.	promoter	1363	1562	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K228819	.	non_covalent_binding_site	1451	1488	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K228819	.	engineered_region	1363	1562	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K228819	.	promoter	1523	1528	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K228819	.	start_codon	1535	1535	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K228819	.	non_covalent_binding_site	1528	1562	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K228819	.	CDS	1363	1450	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K228819	.	promoter	1499	1504	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K228819	.	terminator	1226	1354	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K228819	.	terminator	1314	1354	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K228819	.	engineered_region	1314	1354	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228819	.	stem_loop	1321	1340	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228819	.	stop_codon	1347	1347	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228819	.	polyA_site	1341	1354	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228819	.	terminator	1226	1305	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K228819	.	engineered_region	1226	1305	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228819	.	stem_loop	1237	1280	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228819	.	ribosome_entry_site	47	58	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K228819	.	conserved_region	51	54	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K228819	.	promoter	1	38	.	+	0	ID=BBa_I14033;Name=BBa_I14033
BBa_K228819	.	promoter	4	9	.	+	0	ID=annotation1026172;Name=-35;Parent=BBa_I14033
BBa_K228819	.	operator	1	38	.	+	0	ID=annotation1026174;Name=P(Cat);Parent=BBa_I14033
BBa_K228819	.	promoter	27	32	.	+	0	ID=annotation1026173;Name=-10;Parent=BBa_I14033
BBa_K228819	.	CDS	65	1192	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K228819	.	engineered_tag	1193	1217	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K228819	.	CDS	65	1192	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K228819	.	tag	1154	1192	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K228819	.	engineered_region	65	1192	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
>BBa_K228819
ggcacgtaagaggttccaactttcaccataatgaaacatactagagaaagaggagaaatactagatggtgaatgtgaaa
ccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagcc
acgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaaca
actggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcg
gcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgta
aagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccat
tgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattatt
ttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgg
gcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgat
agcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttccc
actgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtg
cggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacagga
ttttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctg
ttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgatt
cattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagc
ttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgaca
ggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggcttt
acactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagagg
agaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaa
tgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcact
actggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccag
atcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaaga
tgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtatt
gattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagacca
ttatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctt
tcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgg
atgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata