##gff-version 3
##sequence-region BBa_K228820 1 1834
BBa_K228820	.	promoter	895	948	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K228820	.	non_covalent_binding_site	895	913	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K228820	.	non_covalent_binding_site	920	938	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K228820	.	promoter	914	919	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K228820	.	engineered_region	895	948	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K228820	.	promoter	937	942	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K228820	.	terminator	758	886	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K228820	.	terminator	846	886	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K228820	.	polyA_site	873	886	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228820	.	stem_loop	853	872	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228820	.	stop_codon	879	879	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228820	.	engineered_region	846	886	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228820	.	terminator	758	837	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K228820	.	stem_loop	769	812	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228820	.	engineered_region	758	837	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228820	.	promoter	1	38	.	+	0	ID=BBa_I14033;Name=BBa_I14033
BBa_K228820	.	operator	1	38	.	+	0	ID=annotation1026174;Name=P(Cat);Parent=BBa_I14033
BBa_K228820	.	promoter	27	32	.	+	0	ID=annotation1026173;Name=-10;Parent=BBa_I14033
BBa_K228820	.	promoter	4	9	.	+	0	ID=annotation1026172;Name=-35;Parent=BBa_I14033
BBa_K228820	.	ribosome_entry_site	47	58	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K228820	.	conserved_region	51	54	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K228820	.	engineered_region	957	1834	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K228820	.	terminator	1794	1834	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K228820	.	polyA_site	1821	1834	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228820	.	stem_loop	1801	1820	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228820	.	stop_codon	1827	1827	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228820	.	engineered_region	1794	1834	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228820	.	terminator	1706	1785	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K228820	.	stem_loop	1717	1760	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228820	.	engineered_region	1706	1785	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228820	.	ribosome_entry_site	957	971	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K228820	.	engineered_region	957	971	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K228820	.	ribosome_entry_site	964	968	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K228820	.	ribosome_entry_site	957	971	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K228820	.	CDS	978	1697	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K228820	.	CDS	978	1697	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K228820	.	CDS	65	749	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K228820	.	engineered_tag	725	749	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K228820	.	tag	685	718	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K228820	.	CDS	68	684	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
>BBa_K228820
ggcacgtaagaggttccaactttcaccataatgaaacatactagagaaagaggagaaatactagatgtccagattagat
aaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgccc
agaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattga
gatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaa
agttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatg
aaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgt
ggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactact
gatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcc
ttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgcttt
agtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
tactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttga
attagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacc
cttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaat
gctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaag
aactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaataga
atcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacaca
atgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaag
cgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctg
tccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctg
ggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata