LOCUS       BBa_K228822              481 bp    DNA     linear       22-Dec-2024
DEFINITION  lacP(R0010)+SupD(K228001)+terminator(B0015)
ACCESSION   BBa_K228822
VERSION     BBa_K228822.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..344
                     /label=BBa_K228001
     stem_loop       364..407
                     /label=stem_loop
     misc_feature    353..432
                     /label=BBa_B0010
     terminator      353..432
                     /label=BBa_B0010
     stem_loop       448..467
                     /label=T7 TE
     misc_feature    474..474
                     /label=stop
     polyA_site      468..481
                     /label=polya
     misc_feature    441..481
                     /label=BBa_B0012
     terminator      441..481
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagca attcggagag atgccggagc ggctgaacgg
      241 accggtctct aaaaccggag taggggcaac tctaccgggg gttcaaatcc ccctctctcc
      301 gccactacag atccttagcg aaagctaagg atttttttta agcttactag agccaggcat
      361 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      421 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      481 a
//