LOCUS BBa_K228824 4605 bp DNA linear 17-May-2024 DEFINITION AND GATE lacP+SupD+AraC+RBS(B0032)+T7ptag ACCESSION BBa_K228824 VERSION BBa_K228824.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 209..344 /label=BBa_K228001 stem_loop 364..407 /label=stem_loop misc_feature 353..432 /label=BBa_B0010 terminator 353..432 /label=BBa_B0010 stem_loop 448..467 /label=T7 TE misc_feature 474..474 /label=stop polyA_site 468..481 /label=polya misc_feature 441..481 /label=BBa_B0012 terminator 441..481 /label=BBa_B0012 misc_feature 1..481 /label=BBa_K228822 stem_loop 1513..1556 /label=stem_loop misc_feature 1502..1581 /label=BBa_B0010 terminator 1502..1581 /label=BBa_B0010 stem_loop 1597..1616 /label=T7 TE misc_feature 1623..1623 /label=stop polyA_site 1617..1630 /label=polya misc_feature 1590..1630 /label=BBa_B0012 terminator 1590..1630 /label=BBa_B0012 promoter 1639..1768 /label=BBa_I13453 misc_feature 490..527 /label=P(Cat) promoter 493..498 /label=-35 promoter 516..521 /label=-10 misc_feature 490..527 /label=BBa_I14033 misc_feature 536..547 /label=BBa_B0034 misc_feature 540..543 /label=conserved misc_feature 554..1468 /label=BBa_C0080 CDS 554..1429 /label=araC misc_feature 1430..1462 /label=LVA misc_feature 1469..1493 /label=barcode misc_feature 1502..1581 /label=BBa_B0010 stem_loop 1513..1556 /label=stem_loop misc_feature 1590..1630 /label=BBa_B0012 stem_loop 1597..1616 /label=T7 TE polyA_site 1617..1630 /label=polya misc_feature 1623..1623 /label=stop misc_feature 536..1630 /label=BBa_K228006 misc_feature 1639..1768 /label=BBa_I13453 misc_feature 490..1630 /label=BBa_K228008 promoter 1639..1768 /label=BBa_I13453 promoter 490..1768 /label=BBa_K228009 RBS 1777..1789 /label=RBS-3\Weak RBS 1783..1786 /label=RBS misc_feature 1777..1789 /label=BBa_B0032 RBS 1777..1789 /label=BBa_B0032 CDS 1796..4468 /label=cds CDS 1796..4468 /label=BBa_K228000 stem_loop 4488..4531 /label=stem_loop misc_feature 4477..4556 /label=BBa_B0010 terminator 4477..4556 /label=BBa_B0010 stem_loop 4572..4591 /label=T7 TE misc_feature 4598..4598 /label=stop polyA_site 4592..4605 /label=polya misc_feature 4565..4605 /label=BBa_B0012 terminator 4565..4605 /label=BBa_B0012 terminator 4477..4605 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagca attcggagag atgccggagc ggctgaacgg 241 accggtctct aaaaccggag taggggcaac tctaccgggg gttcaaatcc ccctctctcc 301 gccactacag atccttagcg aaagctaagg atttttttta agcttactag agccaggcat 361 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 421 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 481 atactagagt ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga 541 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc 601 gttttatttg atgcctggct ctagtagtga tctacactag cactatcagt gttattaagc 661 tactaaagcg tagttttcgt cgtttgcagc tgacaacttg acggctacat cattcacttt 721 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatactcg 781 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg 841 ggtagtgctc aaaagcagct tcgcctggct gatgcgttgg tcctcgcgcc agcttaagac 901 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca ggcaaacatg 961 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca 1021 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg 1081 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc 1141 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc 1201 cgggcgaaag aaacccgtat tggcaaatat tgacggccag ttaagccatt catgccagta 1261 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc 1321 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc agacaaattc 1381 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt 1441 cattcccagc ggtcggtcga taaaaaaatc gagataacca ttggcctcaa tcggcgttaa 1501 acccgccacc agatgggcgt taaacgagta tcccggcagc aggggatcat tttgcgcttc 1561 agccatctag tatttctcct ctttctctag tatgtttcat tatggtgaaa gttggaacct 1621 cttacgtgcc tactagagac attgattatt tgcacggcgt cacactttgc tatgccatag 1681 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg 1741 tttctccata ccgttttttt gggctagcta ctagagtcac acaggaaagt actagatgac 1801 catgattacc gtgcactaga ataccattaa cattgctaag aacgacttct ctgacatcga 1861 actggctgct atcccgttca acactctggc tgaccattac ggtgagcgtt tagctcgcga 1921 acagttggcc cttgagcatg agtcttacga gatgggtgaa gcacgcttcc gcaagatgtt 1981 tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct gccgccaagc ctctcatcac 2041 taccctactc cctaagatga ttgcacgcat caacgactgg tttgaggaag tgaaagctaa 2101 gcgcggcaag cgcccgacag ccttccagtt cctgtaggaa atcaagccgg aagccgtagc 2161 gtacatcacc attaagacca ctctggcttg cctaaccagt gctgacaata caaccgttca 2221 ggctgtagca agcgcaatcg gtcgggccat tgaggacgag gctcgcttcg gtcgtatccg 2281 tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa caactcaaca agcgcgtagg 2341 gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct gacatgctct ctaagggtct 2401 actcggtggc gaggcgtggt cttcgtggca taaggaagac tctattcatg taggagtacg 2461 ctgcatcgag atgctcattg agtcaaccgg aatggttagc ttacaccgcc aaaatgctgg 2521 cgtagtaggt caagactctg agactatcga actcgcacct gaatacgctg aggctatcgc 2581 aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc caaccttgcg tagttcctcc 2641 taagccgtgg actggcatta ctggtggtgg ctattgggct aacggtcgtc gtcctctggc 2701 gctggtgcgt actcacagta agaaagcact gatgcgctac gaagacgttt acatgcctga 2761 ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg aaaatcaaca agaaagtcct 2821 agcggtcgcc aacgtaatca ccaagtggaa gcattgtccg gtcgaggaca tccctgcgat 2881 tgagcgtgaa gaactcccga tgaaaccgga agacatcgac atgaatcctg aggctctcac 2941 cgcgtggaaa cgtgctgccg ctgctgtgta ccgcaaggac aaggctcgca agtctcgccg 3001 tatcagcctt gagttcatgc ttgagcaagc caataagttt gctaaccata aggccatctg 3061 gttcccttac aacatggact ggcgcggtcg tgtttacgct gtgtcaatgt tcaacccgca 3121 aggtaacgat atgaccaaag gactgcttac gctggcgaaa ggtaaaccaa tcggtaagga 3181 aggttactac tggctgaaaa tccacggtgc aaactgtgcg ggtgtcgata aggttccgtt 3241 ccctgagcgc atcaagttca ttgaggaaaa ccacgagaac atcatggctt gcgctaagtc 3301 tccactggag aacacttggt gggctgagca agattctccg ttctgcttcc ttgcgttctg 3361 ctttgagtac gctggggtac agcaccacgg cctgagctat aactgctccc ttccgctggc 3421 gtttgacggg tcttgctctg gcatccagca cttctccgcg atgctccgag atgaggtagg 3481 tggtcgcgcg gttaacttgc ttcctagtga aaccgttcag gacatctacg ggattgttgc 3541 taagaaagtc aacgagattc tacaagcaga cgcaatcaat gggaccgata acgaagtagt 3601 taccgtgacc gatgagaaca ctggtgaaat ctctgagaaa gtcaagctgg gcactaaggc 3661 actggctggt caatggctgg cttacggtgt tactcgcagt gtgactaagc gttcagtcat 3721 gacgctggct tacgggtcca aagagttcgg cttccgtcaa caagtgctgg aagataccat 3781 tcagccagct attgattccg gcaagggtct gatgttcact cagccgaatc aggctgctgg 3841 atacatggct aagctgattt gggaatctgt gagcgtgacg gtggtagctg cggttgaagc 3901 aatgaactgg cttaagtctg ctgctaagct gctggctgct gaggtcaaag ataagaagac 3961 tggagagatt cttcgcaagc gttgcgctgt gcattgggta actcctgatg gtttccctgt 4021 gtggcaggaa tacaagaagc ctattcagac gcgcttgaac ctgatgttcc tcggtcagtt 4081 ccgcttacag cctaccatta acaccaacaa agatagcgag attgatgcac acaaacagga 4141 gtctggtatc gctcctaact ttgtacacag ccaagacggt agccaccttc gtaagactgt 4201 agtgtgggca cacgagaagt acggaatcga atcttttgca ctgattcacg actccttcgg 4261 taccattccg gctgacgctg cgaacctgtt caaagcagtg cgcgaaacta tggttgacac 4321 atatgagtct tgtgatgtac tggctgattt ctacgaccag ttcgctgacc agttgcacga 4381 gtctcaattg gacaaaatgc cagcacttcc ggctaaaggt aacttgaacc tccgtgacat 4441 cttagagtcg gacttcgcgt tcgcataata ctagagccag gcatcaaata aaacgaaagg 4501 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 4561 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //