LOCUS BBa_K228825 4604 bp DNA linear 22-Nov-2024 DEFINITION AND GATE lacP+SupD+AraC+RBS(J61127)+T7ptag ACCESSION BBa_K228825 VERSION BBa_K228825.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 209..344 /label=BBa_K228001 stem_loop 364..407 /label=stem_loop misc_feature 353..432 /label=BBa_B0010 terminator 353..432 /label=BBa_B0010 stem_loop 448..467 /label=T7 TE misc_feature 474..474 /label=stop polyA_site 468..481 /label=polya misc_feature 441..481 /label=BBa_B0012 terminator 441..481 /label=BBa_B0012 misc_feature 1..481 /label=BBa_K228822 stem_loop 1513..1556 /label=stem_loop misc_feature 1502..1581 /label=BBa_B0010 terminator 1502..1581 /label=BBa_B0010 stem_loop 1597..1616 /label=T7 TE misc_feature 1623..1623 /label=stop polyA_site 1617..1630 /label=polya misc_feature 1590..1630 /label=BBa_B0012 terminator 1590..1630 /label=BBa_B0012 promoter 1639..1768 /label=BBa_I13453 misc_feature 490..527 /label=P(Cat) promoter 493..498 /label=-35 promoter 516..521 /label=-10 misc_feature 490..527 /label=BBa_I14033 misc_feature 536..547 /label=BBa_B0034 misc_feature 540..543 /label=conserved misc_feature 554..1468 /label=BBa_C0080 CDS 554..1429 /label=araC misc_feature 1430..1462 /label=LVA misc_feature 1469..1493 /label=barcode misc_feature 1502..1581 /label=BBa_B0010 stem_loop 1513..1556 /label=stem_loop misc_feature 1590..1630 /label=BBa_B0012 stem_loop 1597..1616 /label=T7 TE polyA_site 1617..1630 /label=polya misc_feature 1623..1623 /label=stop misc_feature 536..1630 /label=BBa_K228006 misc_feature 1639..1768 /label=BBa_I13453 misc_feature 490..1630 /label=BBa_K228008 promoter 1639..1768 /label=BBa_I13453 promoter 490..1768 /label=BBa_K228009 RBS 1777..1788 /label=BBa_J61127 CDS 1795..4467 /label=cds CDS 1795..4467 /label=BBa_K228000 stem_loop 4487..4530 /label=stem_loop misc_feature 4476..4555 /label=BBa_B0010 terminator 4476..4555 /label=BBa_B0010 stem_loop 4571..4590 /label=T7 TE misc_feature 4597..4597 /label=stop polyA_site 4591..4604 /label=polya misc_feature 4564..4604 /label=BBa_B0012 terminator 4564..4604 /label=BBa_B0012 terminator 4476..4604 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagca attcggagag atgccggagc ggctgaacgg 241 accggtctct aaaaccggag taggggcaac tctaccgggg gttcaaatcc ccctctctcc 301 gccactacag atccttagcg aaagctaagg atttttttta agcttactag agccaggcat 361 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 421 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 481 atactagagt ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga 541 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc 601 gttttatttg atgcctggct ctagtagtga tctacactag cactatcagt gttattaagc 661 tactaaagcg tagttttcgt cgtttgcagc tgacaacttg acggctacat cattcacttt 721 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatactcg 781 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg 841 ggtagtgctc aaaagcagct tcgcctggct gatgcgttgg tcctcgcgcc agcttaagac 901 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca ggcaaacatg 961 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca 1021 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg 1081 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc 1141 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc 1201 cgggcgaaag aaacccgtat tggcaaatat tgacggccag ttaagccatt catgccagta 1261 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc 1321 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc agacaaattc 1381 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt 1441 cattcccagc ggtcggtcga taaaaaaatc gagataacca ttggcctcaa tcggcgttaa 1501 acccgccacc agatgggcgt taaacgagta tcccggcagc aggggatcat tttgcgcttc 1561 agccatctag tatttctcct ctttctctag tatgtttcat tatggtgaaa gttggaacct 1621 cttacgtgcc tactagagac attgattatt tgcacggcgt cacactttgc tatgccatag 1681 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg 1741 tttctccata ccgttttttt gggctagcta ctagagaaag agtggaacta ctagatgacc 1801 atgattaccg tgcactagaa taccattaac attgctaaga acgacttctc tgacatcgaa 1861 ctggctgcta tcccgttcaa cactctggct gaccattacg gtgagcgttt agctcgcgaa 1921 cagttggccc ttgagcatga gtcttacgag atgggtgaag cacgcttccg caagatgttt 1981 gagcgtcaac ttaaagctgg tgaggttgcg gataacgctg ccgccaagcc tctcatcact 2041 accctactcc ctaagatgat tgcacgcatc aacgactggt ttgaggaagt gaaagctaag 2101 cgcggcaagc gcccgacagc cttccagttc ctgtaggaaa tcaagccgga agccgtagcg 2161 tacatcacca ttaagaccac tctggcttgc ctaaccagtg ctgacaatac aaccgttcag 2221 gctgtagcaa gcgcaatcgg tcgggccatt gaggacgagg ctcgcttcgg tcgtatccgt 2281 gaccttgaag ctaagcactt caagaaaaac gttgaggaac aactcaacaa gcgcgtaggg 2341 cacgtctaca agaaagcatt tatgcaagtt gtcgaggctg acatgctctc taagggtcta 2401 ctcggtggcg aggcgtggtc ttcgtggcat aaggaagact ctattcatgt aggagtacgc 2461 tgcatcgaga tgctcattga gtcaaccgga atggttagct tacaccgcca aaatgctggc 2521 gtagtaggtc aagactctga gactatcgaa ctcgcacctg aatacgctga ggctatcgca 2581 acccgtgcag gtgcgctggc tggcatctct ccgatgttcc aaccttgcgt agttcctcct 2641 aagccgtgga ctggcattac tggtggtggc tattgggcta acggtcgtcg tcctctggcg 2701 ctggtgcgta ctcacagtaa gaaagcactg atgcgctacg aagacgttta catgcctgag 2761 gtgtacaaag cgattaacat tgcgcaaaac accgcatgga aaatcaacaa gaaagtccta 2821 gcggtcgcca acgtaatcac caagtggaag cattgtccgg tcgaggacat ccctgcgatt 2881 gagcgtgaag aactcccgat gaaaccggaa gacatcgaca tgaatcctga ggctctcacc 2941 gcgtggaaac gtgctgccgc tgctgtgtac cgcaaggaca aggctcgcaa gtctcgccgt 3001 atcagccttg agttcatgct tgagcaagcc aataagtttg ctaaccataa ggccatctgg 3061 ttcccttaca acatggactg gcgcggtcgt gtttacgctg tgtcaatgtt caacccgcaa 3121 ggtaacgata tgaccaaagg actgcttacg ctggcgaaag gtaaaccaat cggtaaggaa 3181 ggttactact ggctgaaaat ccacggtgca aactgtgcgg gtgtcgataa ggttccgttc 3241 cctgagcgca tcaagttcat tgaggaaaac cacgagaaca tcatggcttg cgctaagtct 3301 ccactggaga acacttggtg ggctgagcaa gattctccgt tctgcttcct tgcgttctgc 3361 tttgagtacg ctggggtaca gcaccacggc ctgagctata actgctccct tccgctggcg 3421 tttgacgggt cttgctctgg catccagcac ttctccgcga tgctccgaga tgaggtaggt 3481 ggtcgcgcgg ttaacttgct tcctagtgaa accgttcagg acatctacgg gattgttgct 3541 aagaaagtca acgagattct acaagcagac gcaatcaatg ggaccgataa cgaagtagtt 3601 accgtgaccg atgagaacac tggtgaaatc tctgagaaag tcaagctggg cactaaggca 3661 ctggctggtc aatggctggc ttacggtgtt actcgcagtg tgactaagcg ttcagtcatg 3721 acgctggctt acgggtccaa agagttcggc ttccgtcaac aagtgctgga agataccatt 3781 cagccagcta ttgattccgg caagggtctg atgttcactc agccgaatca ggctgctgga 3841 tacatggcta agctgatttg ggaatctgtg agcgtgacgg tggtagctgc ggttgaagca 3901 atgaactggc ttaagtctgc tgctaagctg ctggctgctg aggtcaaaga taagaagact 3961 ggagagattc ttcgcaagcg ttgcgctgtg cattgggtaa ctcctgatgg tttccctgtg 4021 tggcaggaat acaagaagcc tattcagacg cgcttgaacc tgatgttcct cggtcagttc 4081 cgcttacagc ctaccattaa caccaacaaa gatagcgaga ttgatgcaca caaacaggag 4141 tctggtatcg ctcctaactt tgtacacagc caagacggta gccaccttcg taagactgta 4201 gtgtgggcac acgagaagta cggaatcgaa tcttttgcac tgattcacga ctccttcggt 4261 accattccgg ctgacgctgc gaacctgttc aaagcagtgc gcgaaactat ggttgacaca 4321 tatgagtctt gtgatgtact ggctgatttc tacgaccagt tcgctgacca gttgcacgag 4381 tctcaattgg acaaaatgcc agcacttccg gctaaaggta acttgaacct ccgtgacatc 4441 ttagagtcgg acttcgcgtt cgcataatac tagagccagg catcaaataa aacgaaaggc 4501 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 4561 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //