LOCUS BBa_K228829 4603 bp DNA linear 17-May-2024 DEFINITION AND GATE lacP+SupD+AraC+RBS(B0033)+T7ptag ACCESSION BBa_K228829 VERSION BBa_K228829.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 209..344 /label=BBa_K228001 stem_loop 364..407 /label=stem_loop misc_feature 353..432 /label=BBa_B0010 terminator 353..432 /label=BBa_B0010 stem_loop 448..467 /label=T7 TE misc_feature 474..474 /label=stop polyA_site 468..481 /label=polya misc_feature 441..481 /label=BBa_B0012 terminator 441..481 /label=BBa_B0012 misc_feature 1..481 /label=BBa_K228822 stem_loop 1513..1556 /label=stem_loop misc_feature 1502..1581 /label=BBa_B0010 terminator 1502..1581 /label=BBa_B0010 stem_loop 1597..1616 /label=T7 TE misc_feature 1623..1623 /label=stop polyA_site 1617..1630 /label=polya misc_feature 1590..1630 /label=BBa_B0012 terminator 1590..1630 /label=BBa_B0012 promoter 1639..1768 /label=BBa_I13453 misc_feature 490..527 /label=P(Cat) promoter 493..498 /label=-35 promoter 516..521 /label=-10 misc_feature 490..527 /label=BBa_I14033 misc_feature 536..547 /label=BBa_B0034 misc_feature 540..543 /label=conserved misc_feature 554..1468 /label=BBa_C0080 CDS 554..1429 /label=araC misc_feature 1430..1462 /label=LVA misc_feature 1469..1493 /label=barcode misc_feature 1502..1581 /label=BBa_B0010 stem_loop 1513..1556 /label=stem_loop misc_feature 1590..1630 /label=BBa_B0012 stem_loop 1597..1616 /label=T7 TE polyA_site 1617..1630 /label=polya misc_feature 1623..1623 /label=stop misc_feature 536..1630 /label=BBa_K228006 misc_feature 1639..1768 /label=BBa_I13453 misc_feature 490..1630 /label=BBa_K228008 promoter 1639..1768 /label=BBa_I13453 promoter 490..1768 /label=BBa_K228009 RBS 1777..1787 /label=RBS-4\Weaker RBS 1783..1786 /label=RBS misc_feature 1777..1787 /label=BBa_B0033 RBS 1777..1787 /label=BBa_B0033 CDS 1794..4466 /label=cds CDS 1794..4466 /label=BBa_K228000 stem_loop 4486..4529 /label=stem_loop misc_feature 4475..4554 /label=BBa_B0010 terminator 4475..4554 /label=BBa_B0010 stem_loop 4570..4589 /label=T7 TE misc_feature 4596..4596 /label=stop polyA_site 4590..4603 /label=polya misc_feature 4563..4603 /label=BBa_B0012 terminator 4563..4603 /label=BBa_B0012 terminator 4475..4603 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagca attcggagag atgccggagc ggctgaacgg 241 accggtctct aaaaccggag taggggcaac tctaccgggg gttcaaatcc ccctctctcc 301 gccactacag atccttagcg aaagctaagg atttttttta agcttactag agccaggcat 361 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 421 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 481 atactagagt ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga 541 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc 601 gttttatttg atgcctggct ctagtagtga tctacactag cactatcagt gttattaagc 661 tactaaagcg tagttttcgt cgtttgcagc tgacaacttg acggctacat cattcacttt 721 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatactcg 781 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg 841 ggtagtgctc aaaagcagct tcgcctggct gatgcgttgg tcctcgcgcc agcttaagac 901 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca ggcaaacatg 961 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca 1021 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg 1081 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc 1141 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc 1201 cgggcgaaag aaacccgtat tggcaaatat tgacggccag ttaagccatt catgccagta 1261 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc 1321 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc agacaaattc 1381 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt 1441 cattcccagc ggtcggtcga taaaaaaatc gagataacca ttggcctcaa tcggcgttaa 1501 acccgccacc agatgggcgt taaacgagta tcccggcagc aggggatcat tttgcgcttc 1561 agccatctag tatttctcct ctttctctag tatgtttcat tatggtgaaa gttggaacct 1621 cttacgtgcc tactagagac attgattatt tgcacggcgt cacactttgc tatgccatag 1681 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg 1741 tttctccata ccgttttttt gggctagcta ctagagtcac acaggactac tagatgacca 1801 tgattaccgt gcactagaat accattaaca ttgctaagaa cgacttctct gacatcgaac 1861 tggctgctat cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac 1921 agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg 1981 agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct ctcatcacta 2041 ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc 2101 gcggcaagcg cccgacagcc ttccagttcc tgtaggaaat caagccggaa gccgtagcgt 2161 acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg 2221 ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg 2281 accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc 2341 acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac 2401 tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta ggagtacgct 2461 gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa aatgctggcg 2521 tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag gctatcgcaa 2581 cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta gttcctccta 2641 agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt cctctggcgc 2701 tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac atgcctgagg 2761 tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag 2821 cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc cctgcgattg 2881 agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag gctctcaccg 2941 cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta 3001 tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag gccatctggt 3061 tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag 3121 gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag 3181 gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc 3241 ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc 3301 cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct 3361 ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt 3421 ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat gaggtaggtg 3481 gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta 3541 agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta 3601 ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac 3661 tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga 3721 cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa gataccattc 3781 agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag gctgctggat 3841 acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa 3901 tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg 3961 gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt 4021 ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc ggtcagttcc 4081 gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac aaacaggagt 4141 ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt aagactgtag 4201 tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac tccttcggta 4261 ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg gttgacacat 4321 atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag ttgcacgagt 4381 ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct 4441 tagagtcgga cttcgcgttc gcataatact agagccaggc atcaaataaa acgaaaggct 4501 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 4561 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //