##gff-version 3
##sequence-region BBa_K228830 1 4606
BBa_K228830	.	CDS	1797	4469	.	+	0	ID=BBa_K228000;Name=BBa_K228000
BBa_K228830	.	CDS	1797	4469	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228830	.	terminator	1502	1581	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228830	.	stem_loop	1513	1556	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228830	.	engineered_region	1502	1581	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228830	.	terminator	4478	4606	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K228830	.	terminator	4566	4606	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K228830	.	polyA_site	4593	4606	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228830	.	stem_loop	4573	4592	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228830	.	stop_codon	4599	4599	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228830	.	engineered_region	4566	4606	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228830	.	terminator	4478	4557	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K228830	.	stem_loop	4489	4532	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228830	.	engineered_region	4478	4557	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228830	.	ribosome_entry_site	1777	1790	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_K228830	.	conserved_region	1783	1786	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K228830	.	promoter	490	1768	.	+	0	ID=BBa_K228009;Name=BBa_K228009
BBa_K228830	.	promoter	1639	1768	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228830	.	engineered_region	490	1630	.	+	0	ID=BBa_K228008;Name=BBa_K228008;Parent=BBa_K228009
BBa_K228830	.	polyA_site	1617	1630	.	+	0	ID=annotation2020013;Name=polya;Parent=BBa_K228008
BBa_K228830	.	conserved_region	540	543	.	+	0	ID=annotation2020004;Name=conserved;Parent=BBa_K228008
BBa_K228830	.	promoter	516	521	.	+	0	ID=annotation2020001;Name=-10;Parent=BBa_K228008
BBa_K228830	.	engineered_region	490	527	.	+	0	ID=annotation2020002;Name=BBa_I14033;Parent=BBa_K228008
BBa_K228830	.	engineered_region	536	1630	.	+	0	ID=annotation2020015;Name=BBa_K228006;Parent=BBa_K228008
BBa_K228830	.	tag	1430	1462	.	+	0	ID=annotation2020007;Name=LVA;Parent=BBa_K228008
BBa_K228830	.	engineered_region	1502	1581	.	+	0	ID=annotation2020009;Name=BBa_B0010;Parent=BBa_K228008
BBa_K228830	.	engineered_region	1590	1630	.	+	0	ID=annotation2020011;Name=BBa_B0012;Parent=BBa_K228008
BBa_K228830	.	stop_codon	1623	1623	.	+	0	ID=annotation2020014;Name=stop;Parent=BBa_K228008
BBa_K228830	.	engineered_region	554	1468	.	+	0	ID=annotation2020005;Name=BBa_C0080;Parent=BBa_K228008
BBa_K228830	.	engineered_region	1639	1768	.	+	0	ID=annotation2020016;Name=BBa_I13453;Parent=BBa_K228008
BBa_K228830	.	stem_loop	1597	1616	.	+	0	ID=annotation2020012;Name=T7 TE;Parent=BBa_K228008
BBa_K228830	.	engineered_region	536	547	.	+	0	ID=annotation2020003;Name=BBa_B0034;Parent=BBa_K228008
BBa_K228830	.	operator	490	527	.	+	0	ID=annotation2019999;Name=P(Cat);Parent=BBa_K228008
BBa_K228830	.	promoter	493	498	.	+	0	ID=annotation2020000;Name=-35;Parent=BBa_K228008
BBa_K228830	.	stem_loop	1513	1556	.	+	0	ID=annotation2020010;Name=stem_loop;Parent=BBa_K228008
BBa_K228830	.	CDS	554	1429	.	+	0	ID=annotation2020006;Name=araC;Parent=BBa_K228008
BBa_K228830	.	engineered_region	1469	1493	.	+	0	ID=annotation2020008;Name=barcode;Parent=BBa_K228008
BBa_K228830	.	promoter	1639	1768	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K228009
BBa_K228830	.	terminator	1590	1630	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228830	.	polyA_site	1617	1630	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228830	.	stem_loop	1597	1616	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228830	.	stop_codon	1623	1623	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228830	.	engineered_region	1590	1630	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228830	.	engineered_region	1	481	.	+	0	ID=BBa_K228822;Name=BBa_K228822
BBa_K228830	.	terminator	353	432	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K228822
BBa_K228830	.	stem_loop	364	407	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228830	.	engineered_region	353	432	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228830	.	mature_transcript_region	209	344	.	+	0	ID=BBa_K228001;Name=BBa_K228001;Parent=BBa_K228822
BBa_K228830	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K228822
BBa_K228830	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K228830	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K228830	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K228830	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K228830	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K228830	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K228830	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K228830	.	terminator	441	481	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K228822
BBa_K228830	.	polyA_site	468	481	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228830	.	stem_loop	448	467	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228830	.	stop_codon	474	474	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228830	.	engineered_region	441	481	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K228830
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagcaattcggagagatgccggagcggctgaa
cggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatcctt
agcgaaagctaaggattttttttaagcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttatatactagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccga
caaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatc
tacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatc
attcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatag
agttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggc
tgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcga
caggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcg
cgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagat
ttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcgg
ctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcg
cgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcg
ggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgag
attgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaa
cccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcct
ctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcg
tcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctac
tgtttctccataccgtttttttgggctagctactagagtcacacaggaaacctactagatgaccatgattaccgtgcac
tagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgacc
attacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaa
gatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccct
aagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcc
tgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatac
aaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaa
gctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaag
ttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattca
tgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagta
ggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggca
tctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacgg
tcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtg
tacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcacca
agtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacat
gaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgt
atcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggact
ggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaa
aggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccg
ttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacactt
ggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgag
ctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgag
gtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacg
agattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctc
tgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgt
tcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagcta
ttgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgt
gagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaa
gataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcagg
aatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaa
caaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccac
cttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtacca
ttccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggc
tgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaac
ttgaacctccgtgacatcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttata