LOCUS       BBa_K228850              978 bp    DNA     linear       22-Nov-2024
DEFINITION  PO-GFP
ACCESSION   BBa_K228850
VERSION     BBa_K228850.1  
FEATURES             Location/Qualifiers
     promoter        1..92
                     /label=PO
     promoter        1..92
                     /label=BBa_I746361
     RBS             101..115
                     /label=RBS-1\Strong
     RBS             108..112
                     /label=RBS
     misc_feature    101..115
                     /label=BBa_B0030
     RBS             101..115
                     /label=BBa_B0030
     CDS             122..841
                     /label=GFP protein
     CDS             122..841
                     /label=BBa_E0040
     stem_loop       861..904
                     /label=stem_loop
     misc_feature    850..929
                     /label=BBa_B0010
     terminator      850..929
                     /label=BBa_B0010
     stem_loop       945..964
                     /label=T7 TE
     misc_feature    971..971
                     /label=stop
     polyA_site      965..978
                     /label=polya
     misc_feature    938..978
                     /label=BBa_B0012
     terminator      938..978
                     /label=BBa_B0012
ORIGIN
        1 cgcgccccgc gattcgctaa ggtgctgttg tgtcagtgat aagccatccg ggactgatgg
       61 cggaggatgc gcatcgtcgg gaaactgatg cctactagag attaaagagg agaaatacta
      121 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      181 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      241 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      301 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      361 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      421 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      481 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      541 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      601 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      661 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      721 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      781 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
      841 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      961 ggcctttctg cgtttata
//