LOCUS BBa_K228853 4380 bp DNA linear 22-Nov-2024 DEFINITION AND GATE Sal+SupD+LacP+RBS(B0030)+T7ptag ACCESSION BBa_K228853 VERSION BBa_K228853.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS-1\Strong RBS 216..220 /label=RBS misc_feature 209..223 /label=BBa_B0030 RBS 209..223 /label=BBa_B0030 CDS 230..2902 /label=cds CDS 230..2902 /label=BBa_K228000 stem_loop 2922..2965 /label=stem_loop misc_feature 2911..2990 /label=BBa_B0010 terminator 2911..2990 /label=BBa_B0010 stem_loop 3006..3025 /label=T7 TE misc_feature 3032..3032 /label=stop polyA_site 3026..3039 /label=polya misc_feature 2999..3039 /label=BBa_B0012 terminator 2999..3039 /label=BBa_B0012 CDS 3048..3950 /label=NahR promoter 3951..4040 /label=Pr promoter promoter 4029..4097 /label=pSal promoter 3048..4099 /label=BBa_K228004 misc_feature 4108..4243 /label=BBa_K228001 stem_loop 4263..4306 /label=stem_loop misc_feature 4252..4331 /label=BBa_B0010 terminator 4252..4331 /label=BBa_B0010 stem_loop 4347..4366 /label=T7 TE misc_feature 4373..4373 /label=stop polyA_site 4367..4380 /label=polya misc_feature 4340..4380 /label=BBa_B0012 terminator 4340..4380 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgaccatgat 241 taccgtgcac tagaatacca ttaacattgc taagaacgac ttctctgaca tcgaactggc 301 tgctatcccg ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt 361 ggcccttgag catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg 421 tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct 481 actccctaag atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg 541 caagcgcccg acagccttcc agttcctgta ggaaatcaag ccggaagccg tagcgtacat 601 caccattaag accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt 661 agcaagcgca atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct 721 tgaagctaag cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt 781 ctacaagaaa gcatttatgc aagttgtcga ggctgacatg ctctctaagg gtctactcgg 841 tggcgaggcg tggtcttcgt ggcataagga agactctatt catgtaggag tacgctgcat 901 cgagatgctc attgagtcaa ccggaatggt tagcttacac cgccaaaatg ctggcgtagt 961 aggtcaagac tctgagacta tcgaactcgc acctgaatac gctgaggcta tcgcaacccg 1021 tgcaggtgcg ctggctggca tctctccgat gttccaacct tgcgtagttc ctcctaagcc 1081 gtggactggc attactggtg gtggctattg ggctaacggt cgtcgtcctc tggcgctggt 1141 gcgtactcac agtaagaaag cactgatgcg ctacgaagac gtttacatgc ctgaggtgta 1201 caaagcgatt aacattgcgc aaaacaccgc atggaaaatc aacaagaaag tcctagcggt 1261 cgccaacgta atcaccaagt ggaagcattg tccggtcgag gacatccctg cgattgagcg 1321 tgaagaactc ccgatgaaac cggaagacat cgacatgaat cctgaggctc tcaccgcgtg 1381 gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag 1441 ccttgagttc atgcttgagc aagccaataa gtttgctaac cataaggcca tctggttccc 1501 ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa 1561 cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta 1621 ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc cgttccctga 1681 gcgcatcaag ttcattgagg aaaaccacga gaacatcatg gcttgcgcta agtctccact 1741 ggagaacact tggtgggctg agcaagattc tccgttctgc ttccttgcgt tctgctttga 1801 gtacgctggg gtacagcacc acggcctgag ctataactgc tcccttccgc tggcgtttga 1861 cgggtcttgc tctggcatcc agcacttctc cgcgatgctc cgagatgagg taggtggtcg 1921 cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa 1981 agtcaacgag attctacaag cagacgcaat caatgggacc gataacgaag tagttaccgt 2041 gaccgatgag aacactggtg aaatctctga gaaagtcaag ctgggcacta aggcactggc 2101 tggtcaatgg ctggcttacg gtgttactcg cagtgtgact aagcgttcag tcatgacgct 2161 ggcttacggg tccaaagagt tcggcttccg tcaacaagtg ctggaagata ccattcagcc 2221 agctattgat tccggcaagg gtctgatgtt cactcagccg aatcaggctg ctggatacat 2281 ggctaagctg atttgggaat ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa 2341 ctggcttaag tctgctgcta agctgctggc tgctgaggtc aaagataaga agactggaga 2401 gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca 2461 ggaatacaag aagcctattc agacgcgctt gaacctgatg ttcctcggtc agttccgctt 2521 acagcctacc attaacacca acaaagatag cgagattgat gcacacaaac aggagtctgg 2581 tatcgctcct aactttgtac acagccaaga cggtagccac cttcgtaaga ctgtagtgtg 2641 ggcacacgag aagtacggaa tcgaatcttt tgcactgatt cacgactcct tcggtaccat 2701 tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg acacatatga 2761 gtcttgtgat gtactggctg atttctacga ccagttcgct gaccagttgc acgagtctca 2821 attggacaaa atgccagcac ttccggctaa aggtaacttg aacctccgtg acatcttaga 2881 gtcggacttc gcgttcgcat aatactagag ccaggcatca aataaaacga aaggctcagt 2941 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 3001 acactggctc accttcgggt gggcctttct gcgtttatat actagagtca atccgtaaac 3061 aggtcaaaca tcagttgccg caaccaaata ttggctaggt ccttgtggta cttcgcatgc 3121 cagaacatgt tgatggctat ttcaggcaag acgactgggt gcggcaaggc gcttaggccg 3181 aagggctcca cgcagcagtc ggctaaacgt atcggcacag tggcgagcag atcggtgcgc 3241 tggaggatgt ggccaacggc ggcgaagtgc ggcacttcca gacggatgtc gcgccggatg 3301 ccgacccgtg tcatgtacgt gtccacctcg ccgtggccgg tgccagcggc gatgacacgc 3361 acgtggccgt aggaacagaa gcgctccaga gtcaggggtt cgcgggtgac tggatggtcc 3421 ttgcgacata ggcacacgta gtgattctgg agcagccggc gctgaaagaa gccagtttgc 3481 agattgggaa gcaggcccac ggccaagtcc acggttccgt tctgcaaggc ctgcatcagg 3541 ctcatcgaac tgtcgcgcac cgtactgatc acgcaattgg gggcctggtg agccagcaca 3601 tccatcagcc gcggcatgaa gtagatctcg ccaatgtcgg tcatggccag ggtgaaggta 3661 cgctcgctgg tcagcggatc gaagctttca tggtgctgta gggcgttgcg cagtgcgtgc 3721 atggccgaag tgacgggctc ggccagatgc gcggcatagg gtgtgggttc cattccctga 3781 tgtgtgcgca cgaagagtgg gtcctgtagc gaggtgcgca ggcgtttcag cgcattgctc 3841 acggcaggct gggtcaggcc caggttctcc gcagtgatag agacgcgtct gtcgaccagc 3901 aactggttga acaccaccag caggtttaaa tccaggtcac gcagttccat ggggcctcgc 3961 ttgggttatt gctggtgccc ggccgggcgc aatattcatg ttgatgattt attatatatc 4021 gagtggtgta tttatcaata ttgtttgctc cgttatcgtt attaacaagt catcaataaa 4081 gccatcacga gtaccatagt actagagcaa ttcggagaga tgccggagcg gctgaacgga 4141 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg 4201 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gccaggcatc 4261 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 4321 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //