##gff-version 3
##sequence-region BBa_K228853 1 4380
BBa_K228853	.	terminator	4252	4331	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228853	.	stem_loop	4263	4306	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228853	.	engineered_region	4252	4331	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228853	.	promoter	3048	4099	.	+	0	ID=BBa_K228004;Name=BBa_K228004
BBa_K228853	.	CDS	3048	3950	.	+	0	ID=annotation2075285;Name=NahR;Parent=BBa_K228004
BBa_K228853	.	promoter	4029	4097	.	+	0	ID=annotation2075287;Name=pSal;Parent=BBa_K228004
BBa_K228853	.	promoter	3951	4040	.	+	0	ID=annotation2075286;Name=Pr promoter;Parent=BBa_K228004
BBa_K228853	.	terminator	2911	2990	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228853	.	stem_loop	2922	2965	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228853	.	engineered_region	2911	2990	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228853	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K228853	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K228853	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K228853	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K228853	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K228853	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K228853	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K228853	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K228853	.	terminator	4340	4380	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228853	.	stem_loop	4347	4366	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228853	.	engineered_region	4340	4380	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228853	.	stop_codon	4373	4373	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228853	.	polyA_site	4367	4380	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228853	.	mature_transcript_region	4108	4243	.	+	0	ID=BBa_K228001;Name=BBa_K228001
BBa_K228853	.	CDS	230	2902	.	+	0	ID=BBa_K228000;Name=BBa_K228000
BBa_K228853	.	CDS	230	2902	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228853	.	terminator	2999	3039	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228853	.	stem_loop	3006	3025	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228853	.	engineered_region	2999	3039	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228853	.	stop_codon	3032	3032	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228853	.	polyA_site	3026	3039	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228853	.	ribosome_entry_site	209	223	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K228853	.	ribosome_entry_site	216	220	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K228853	.	engineered_region	209	223	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K228853	.	ribosome_entry_site	209	223	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_K228853
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgaccat
gattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaac
actctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaag
cacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcac
taccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgaca
gccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaacca
gtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtat
ccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaa
gcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataagg
aagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaa
tgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggt
gcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggct
attgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgttta
catgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgcc
aacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccgg
aagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcg
caagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttccct
tacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgc
ttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgt
cgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctcca
ctggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagc
accacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgat
gctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgct
aagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaaca
ctggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcag
tgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagatacc
attcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctga
tttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggc
tgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttc
cctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagccta
ccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagcca
agacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgac
tccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtctt
gtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttcc
ggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagtcaatccgtaaacaggtcaaacatcagttgccgc
aaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggt
gcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcg
ctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtac
gtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggg
gttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagt
ttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcg
cgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaa
tgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcag
tgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacg
aagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccg
cagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccat
ggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgt
atttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagtactagagc
aattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaat
ccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata